A STUDY OF SUBCRITICAL WATER AND ETHANOLIC EXTRACTS OF ROSEMARY LEAVES (ROSMARINUS OFFICINALIS L.) BY GC-MS

UDK 543.612.3

  • Galina Ilyinichna Vdovina Samara State National Research University named after Academician S.P. Korolev Email: galina-vdovina@mail.ru
  • Larisa Viktorovna Pavlova Samara State National Research University named after Academician S.P. Korolev Email: pavlova.lv@ssau.ru
  • Igor Artemyevich Platonov Samara State National Research University named after Academician S.P. Korolev Email: platonov.ia@ssau.ru
  • Vladimir Aleksandrovich Kurkin Samara State Medical University Email: kurkinvladimir@yandex.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20240213180
Keywords: extraction, subcritical water, water-ethanol extract, rosemary, GC-MS

Abstract

Extraction of biologically active substances from the leaves of rosemary (Rosmarinus officinalis L.) is carried out by solutions of ethanol in water with 95, 70, 50, 10% ethanol concentration by maceration, as well as by boiling under reflux using hot water and subcritical water at the temperature 130, 150 °С and a pressure of 5±0.1 MPa in dynamic mode.

 The extracts were analyzed by gas chromatography with mass spectrometric detection by direct injection, as well as after derivatization with N,O-bistrimethylsilyltrifluoroacetamide. About 100 components have been identified in rosemary leaf extracts, phenolic compounds and carboxylic acids are dominant. The main volatile organic compounds (VOCs) are terpenoids: α-terpineol, borneol, camphor, α-pinene, β-pinene, myrcene, aromatic compounds: p-vinyl guaiacol, cymene. The dominant non-volatile components of all extracts include phenolcarboxylic acids: rosmarinic acid, caffeic acid, benzoic acid, carboxylic acids: malonic acid, caproic acid, glutaric acid, palmitic acid, and carbohydrates: raffinose, D-mannopyranose, D-fructose. As the temperature of the dynamic water extraction process increases, the extraction efficiency of non-volatile components increases. In terms of the number of extracted compounds, the efficiency of extraction with subcritical water is comparable to extraction with 95% ethanol concentration by the maceration method.

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Author Biographies

Galina Ilyinichna Vdovina , Samara State National Research University named after Academician S.P. Korolev

student

Larisa Viktorovna Pavlova , Samara State National Research University named after Academician S.P. Korolev

Head of the laboratory of the Department of Chemistry, Candidate of Chemical Sciences, Associate Professor

Igor Artemyevich Platonov , Samara State National Research University named after Academician S.P. Korolev

Head of the Department of Chemistry, Doctor of Technical Sciences, Professor

Vladimir Aleksandrovich Kurkin , Samara State Medical University

Head of the Department of Pharmacognosy with Botany and Basics of Herbal Medicine, Doctor of Pharmaceutical Sciences, Professor

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Rosmarinic acid, GC-MS. URL: https://pubchem.ncbi.nlm.nih.gov/compound/Rosmarinic-acid#section=GC-MS.

Published
2024-06-17
How to Cite
1. Vdovina G. I., Pavlova L. V., Platonov I. A., Kurkin V. A. A STUDY OF SUBCRITICAL WATER AND ETHANOLIC EXTRACTS OF ROSEMARY LEAVES (ROSMARINUS OFFICINALIS L.) BY GC-MS // chemistry of plant raw material, 2024. № 2. P. 320-328. URL: http://journal.asu.ru/cw/article/view/13180.
Issue
No 2 (2024)
Section
Low-molecular weight compounds

Copyright (c) 2024 chemistry of plant raw material

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