SCREENING OF PARAMETERS FOR THE EXTRACTION OF BIOLOGICALLY ACTIVE SUBSTANCES FROM TAGETES PATULA AND ASSESSMENT OF THE ANTIOXIDANT ACTIVITY OF THEIR AQUEOUS EXTRACTS

UDC 615.322

  • Kamila Shukhratovna Kazimova A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of the Russian Academy of Sciences Email: camilakazimova@yandex.ru
  • Farida Yunusovna Akhmadullina Kazan National Research Technological University Email: akhmadullina1951@mail.ru
  • Anton Nikolaevich Akulov Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center of the Russian Academy of Sciences Email: akulov_anton@mail.ru
  • Yulia Vladimirovna Shcherbakova Kazan National Research Technological University Email: balakirevajulia3@mail.ru
  • Evgeny Nikolaevich Nikitin A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of the Russian Academy of Sciences Email: berkut@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20240313383
Keywords: extraction, technological factors, aqueous phytoextracts, antioxidant activity, HPLC analysis

Abstract

The influence of various technological factors on the efficiency of extraction of water-soluble extractive substances from dry inflorescences of marigolds, prostrate (Tagetes patula), including temperature, mixing intensity, hydromodulus, and extraction duration, was studied. A rational mode of the extraction process was established based on the given characteristics of the integral antioxidant activity of aqueous phytoextracts, correlated to a unit of extractive substances released in each experiment. The value of the integral antioxidant activity was determined by coulometric titration with electrogenerated bromine using rutin as a standard. The best results (71–75 mg/100 ml of extract) were obtained under the following process conditions: temperature 70 °C, stirring intensity 500 rpm, hydromodulus 1 : 300, phytomaterial particle size 5 mm, and extraction time 90 min. HPLC analysis of an aqueous extract of marigold inflorescences revealed the presence of two groups of phenolic compounds: phenolic acids and flavonoids. Among phenolic acids, gallic, chlorogenic, caffeic, coumaric, ferulic and synapic acids, as well as flavonoids rutin and quercetin were identified.

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Author Biographies

Kamila Shukhratovna Kazimova , A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of the Russian Academy of Sciences

Junior Researcher

Farida Yunusovna Akhmadullina , Kazan National Research Technological University

Senior Lecturer

Anton Nikolaevich Akulov , Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center of the Russian Academy of Sciences

Senior Researcher

Yulia Vladimirovna Shcherbakova , Kazan National Research Technological University

Associate Professor

Evgeny Nikolaevich Nikitin , A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of the Russian Academy of Sciences

Head of Laboratory

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Published
2024-10-01
How to Cite
1. Kazimova K. S., Akhmadullina F. Y., Akulov A. N., Shcherbakova Y. V., Nikitin E. N. SCREENING OF PARAMETERS FOR THE EXTRACTION OF BIOLOGICALLY ACTIVE SUBSTANCES FROM TAGETES PATULA AND ASSESSMENT OF THE ANTIOXIDANT ACTIVITY OF THEIR AQUEOUS EXTRACTS // chemistry of plant raw material, 2024. № 3. P. 250-258. URL: http://journal.asu.ru/cw/article/view/13383.
Issue
No 3 (2024)
Section
Low-molecular weight compounds

Copyright (c) 2024 chemistry of plant raw material

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