QUANTITATIVE ANALYSIS OF FLAVONOIDS IN CAMOMILE FLOWERS (MATRICARIA CHAMOMILLA L.) BY MICROCOLUMN HPLC-UV
Abstract
A reverse-phase microcolumn HPLC method with UV detection (330 nm) was developed for quantitative analysis of flavonoids of M. chamomilla flowers using a ProntoSIL-120-5-C18 AQ column (60 mm × 1 mm × 5 μm), gradient elution system 0,2 M LiClO4/0,006 M HClO4–acetonitrile and cosmosiin as a reference compound. Optimal conditions for the hydrolytic process of flavonoids (KOH concentration 0,25%, extraction time 30 min) and the parameters of flavonoids extraction (particle size 0,25 mm, extraction temperature 60 °C, a single extraction step lasting 30 min at a ratio 1:100) were selected. Validation analysis showed that the proposed method is characterized by satisfactory metrological parameters. The limit of detection (LOD) and limit of quantification (LOQ) of cosmosiin were 84 and 255 ng/ml, respectively. The accuracy for cosmosiin content levels 80–120% was less than 101,93–103,00%. The method was used for the analysis of introduced and commercial samples of M. chamomilla flowers.Downloads
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Kurkin V.A., Sharova O.V., Afanas'eva P.V., Vel'miseva L.E., Fedorov A.V. Izvestiia Samarskogo nauchnogo tsentra RAN, 2012, vol. 14, pp. 2249–2252. (in Russ.).
Petruĺová-Poracká V., Repčák M., Vilková M., Imrich J. Food Chem., 2013, vol. 141, pp. 54–59.
Ganzera M., Guggenberger M., Stuppner H., Zidorn C. Planta Med., 2008, vol. 74, pp. 453–457.
Caleja C., Barros M., Antonoi A.L., Ciric A., Barreira J.C.M., Sokovic M., Oliveira M.B.P.P., Santos-Buelga C., Fer-reira I.C.F.R. J. Funct. Foods., 2015, vol. 16, pp. 114–124.
Kunde R., Isaak O. Planta Med., 1979, vol. 37, pp. 124–130.
Exner J., Reichling J., Becker H. Planta Med., 1980, vol. 39, pp. 219–219.
Xie X.-Y., Wang R., Shi Y.-P. Chem. Nat. Comp., 2014, vol. 50, pp. 910–911.
Redaelli C., Santaniello L.F. J. Chromatogr. A., 1981, vol. 209, pp. 110–112.
Glasl H. J. Chromatogr A., 1975, vol. 114, pp. 215–219.
Uteshev B.S., Laskova I.L., Afanas’ev V.A. Eksp. Klin. Farmakol., 1999, vol. 62, pp. 52–55.
Füller E., Blaschek W., Franz G. Planta Med., 1991, vol. 57, pp. 40–40.
Rezaeih R.A.P., Gurbuz B., Uyanik M., Rahimi A., Arslan N. J. Essent. Oil Bear. Pl., 2015, vol. 18, pp. 255–260.
Miguel F.G., Cavalheiro A.H., Spinola N.F., Ribeiro D.L., Barcelos G.R.M., Antunes L.M.G., Hori J.I., Marquele-Oliveira F., Rocha B.A., Berretta A.A. J. Evid. Based Complement. Altern. Med., 2015, vol. 2015, URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4573433/
Li K.-C., Ho Y.-L., Hsieh W.-T., Huang S.-S., Chang Y.-S., Huang G.-J. Int. J. Mol. Sci., 2015, vol. 16, pp. 1736–1754.
Achterrath-Tuckermann U., Kunde R., Flaskamp E., Isaac O., Thiemer K. Planta Med., 1980, vol. 39, pp. 38–50.
Maschi O., Cero E.D., Galli G.V., Caruso D., Bosisio E., Dell’Agli M. J. Agric. Food Chem., 2008, vol. 56, pp. 5015–5020.
Gosudarstvennaia Farmakopeia SSSR. [State Pharmacopoeia of the USSR]. Moscow, 1990, vol. 2, pp. 237–238. (in Russ.).
Romashki tsvetki. Gosudarstvennaia Farmakopeia Respubliki Belarus'. [Chamomile flowers. State Pharmacopoeia of the Republic of Belarus]. Minsk, 2007, vol. II, pp. 412–413. (in Russ.).
Romashki tsvety. Gosudarstvennaia Farmakopeia Respubliki Kazakhstan. [Chamomile flowers. State Pharmacopoeia of the Republic of Kazakhstan]. Almaty, 2008, vol. 2, 724 p. (in Russ.).
United States Pharmacopeia. “Dietary supplements—Botanicals”. The United States Pharmacopeial Convention, 2007, 901 p.
Pecherskaia L.G., Rogozkina N.L. Razrabotka, issledovanie i marketing novoi farmatsevticheskoi promyshlennosti: sbornik nauchnykh trudov. [Development, research and marketing of a new pharmaceutical industry: a collection of scientific papers]. Piatigorsk, 2005, pp. 44–46. (in Russ.).
Bubenchikova V.N., Kondratova Iu.A. Kubanskii nauchno-meditsinskii vestnik, 2006, vol. 10, pp. 19–21. (in Russ.).
Redaelli C., Formentini L., Santaniello E. Planta Med., 1981, vol. 42, pp. 288–292.
Zeković Z., Pekić B., Lepojević Ž., Petrović L. Chromatographia, 1994, vol. 39, pp. 587–590.
Raal A., Orav A., Pussa T., Valner C., Malmiste B., Arak E. Food Chem., 2012, vol. 131, pp. 632–638.
Mulinacci M., Romani A., Pinelli P., Vincieri F.F., Prucher D. Chromatographia, 2000, vol. 51, pp. 301–307.
Weber B., Herrmann M., Hartmann B., Joppe H., Schmidt C.O., Bertram H.J. Eur. Food Res. Technol., 2008, vol. 226, pp. 755–760.
Haghi G., Hatami A., Safaei A., Mehran M. Res. Pharm. Sci., 2014, vol. 9, pp. 31–37.
Nováková L., Vildová A., Mateus J.P., Gonçalves T., Solich P. Talanta, 2010, vol. 82, pp. 1271–1280.
Poludennyi L.V., Sotnik V.F., Khlaptsev E.E. Efirnomaslichnye i lekarstvennye rasteniia. [Aromatic and medicinal plants]. Moscow, 1979, 167 p. (in Russ.).
Olennikov D.N., Kashchenko N.I. Chem. Nat. Comp., 2016, vol. 52, pp. 992–995.
Rukovodstvo dlia predpriiatii farmatsevticheskoi promyshlennosti (metodicheskie rekomendatsii). [Guide for the pharmaceutical industry enterprises (guidelines)]. Moscow, 2007, 192 p. (in Russ.).
Matricaria flower. Art. No 01/2008:0404. European Pharmacopoeia 7.0, 2008, pp. 1178–1179.
Grotewold E. The Science of Flavonoids. New York, 2006, 49 p.
Švehlíková V., Bennett R.N., Mellon F.A., Needs P.W., Piacente S., Kroon P.A., Bao Y. Phytochemistry, 2004, vol. 65, pp. 2323–2332.
Tschiersch K., Hoelzl J. Pharm. Z., 1992, vol. 137, pp. 208–214.
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