DEVELOPMENT AND VALIDATION OF METHODS OF QUANTITATIVE DETERMINATION OF FLAVONOIDS IN THE FLOWERS OF FORSYTHIA INTERMEDIATE (FORSYTHIA INTERMEDIA ZABEL.)
Abstract
As object of research used the flowers of forsythia intermediate, which are widely used in folk medicine for diseases of the liver and kidney. The purpose of work was the development and validation of methods to quantify of the sum flavonoids of the flowers of forsythia intermediate. Are carried out researches by definition optimum extragent as which 30% spirit ethyl have been chosen. Previous pharmacological studies have established that an extract from the flowers of forsythia exhibits a pronounced hepatoprotective effect. The method of differential spectrophotometry by complexation reaction of aluminum chloride in investigated object installs the quantitative maintenance of the sum flavonoides in recalculation on routines (5,96±0,14%). Validation evaluation of the proposed method carried out on such parameters as specificity, linearity, precision (repeatability), correctness. Validation showed that all the studied validation parameters are within acceptance criteria. The proposed technique by the authors can be included in the standard documentation on the flowers of forsythia intermediate.Downloads
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References
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