CHEMICAL CHARACTERIZATION OF THE SAUSSUREA SALICIFOLIA L. POLYSACCHARIDE COMPLEX AND ITS NO-STIMULATING PROPERTIES

UDC 581.192.2

  • Yekaterina Igorevna Gulina Siberian State Medical University Email: e.gulina1@gmail.com
  • Anastasiya Vasil'yevna Zykova Siberian State Medical University Email: anastasya.zykova@mail.ru
  • Anastasiya Aleksandrovna Ligacheva Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences Email: ligacheva_aa@pharmso.ru
  • Marina Grigor'yevna Danilets Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences Email: m.danilets@mail.ru
  • Yevgeniya Sergeyevna Trofimova Siberian State Medical University; Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences Email: trofimova_es@pharmso.ru
  • Natal'ya Sergeyevna Selivanova Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences Email: selivanova_ns@pharmso.ru
  • Evgeniy Yur'yevich Sherstoboev Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences Email: sherstoboev_eu@pharmso.ru
  • Yelizaveta Aleksandrovna Gorobec Siberian State Medical University Email: elizabetta.gor777@yandex.ru
  • Sergey Vladimirovich Krivoshchekov Siberian State Medical University Email: ksv_tsu@mail.ru
  • Mikhail Valer'yevich Belousov Siberian State Medical University Email: mvb63@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20230413545
Keywords: Saussurea salicifolia L., polysaccharides, ion exchange chromatography, monomeric composition, molecular weight distribution, congo red, IR, NO, endotoxin

Abstract

A polysaccharide complex (PSC) from the aboveground part of Saussurea salicifolia L. (SS) was isolated and studied. SS PSC contains 54.99±0.53% hexose, 12.99±0.26% uronic acids, 5.24±0.04% protein, neutral monosaccharides are represented by glucose, galactose, xylose and rhamnose (molar ratios: 1,67 : 1,00 : 1,20 : 1,42). Five main components were isolated using ion exchange chromatography. The structure of the obtained polysaccharides (PS) 1-5 was characterized using physicochemical methods, such as IR spectroscopy, high-efficiency exclusive and gas-liquid chromatography, reactions with congo red. All PS are characterized by a different content of hexoses (from 32.46±2.63 to 83.64± 5.93%), uronic acids (from 2.56± 0.17 to 20.07± 3.70%) and a slight admixture of protein (from 0.75±0.13 to 2.28± 0.17%). The polysaccharides obtained are highly homogeneous samples, different in monomeric composition. The major component of SS-1 PS is represented by galactose, SS-2, 3, 4 PS – rhamnose, and SS-5 PS – glucose and xylose. The triple helix structure is installed for SS PSC and PS SS-5. All isolated PS stimulated NO synthase of peritoneal macrophages at control level 2 (LPS), and samples 3, 4 and 5 showed endotoxin-independent activity.

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Author Biographies

Yekaterina Igorevna Gulina, Siberian State Medical University

ассистент кафедры фармацевтического анализа

Anastasiya Vasil'yevna Zykova, Siberian State Medical University

аспирант кафедры фармацевтического анализа, кандидат химических наук

Anastasiya Aleksandrovna Ligacheva, Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences

научный сотрудник отдела иммунофармакологии, кандидат биологических наук

Marina Grigor'yevna Danilets, Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences

заведующий отделом экспериментальных биологических моделей, доктор биологических наук

Yevgeniya Sergeyevna Trofimova , Siberian State Medical University; Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences

старший научный сотрудник отдела иммунофармакологии, кандидат медицинских наук

Natal'ya Sergeyevna Selivanova , Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences

младший научный сотрудник отдела иммунофармакологии

Evgeniy Yur'yevich Sherstoboev , Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences

заведующий отделом иммунофармакологии, доктор медицинских наук, профессор

Yelizaveta Aleksandrovna Gorobec, Siberian State Medical University

студентка 5 курса фармацевтического факультета, кандидат химических наук

Sergey Vladimirovich Krivoshchekov, Siberian State Medical University

доцент кафедры фармацевтического анализа, кандидат химических наук

Mikhail Valer'yevich Belousov, Siberian State Medical University

заведующий кафедрой фармацевтического анализа, доктор фармацевтических наук, профессор

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Published
2023-12-15
How to Cite
1. Gulina Y. I., Zykova A. V., Ligacheva A. A., Danilets M. G., Trofimova Y. S., Selivanova N. S., Sherstoboev E. Y., Gorobec Y. A., Krivoshchekov S. V., Belousov M. V. CHEMICAL CHARACTERIZATION OF THE SAUSSUREA SALICIFOLIA L. POLYSACCHARIDE COMPLEX AND ITS NO-STIMULATING PROPERTIES // chemistry of plant raw material, 2023. № 4. P. 99-109. URL: http://journal.asu.ru/cw/article/view/13545.
Issue
No 4 (2023)
Section
Biopolymers of plants

Copyright (c) 2023 chemistry of plant raw material

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