THE EFFECT OF EXTRACTANTS ON THE EFFICIENCY OF EXTRACTION OF WATER-SOLUBLE POLYSACCHARIDES AND FREE CARBOHYDRATES IN MEDICINAL PLANT RAW MATERIALS OF PLANTAIN

UDC 54.056. 543.422.3

  • Tatyana Mikhailovna Grebenkina Samara Federal Research Center of the Russian Academy of Sciences, Institute of Ecology of the Volga Basin of the Russian Academy of Sciences Email: gre92ta@gmail.com
DOI_disc_logo https://doi.org/10.14258/jcprm.20240313746
Keywords: free carbohydrates, polysaccharides, conversion of raw materials, picric acid

Abstract

Establishing optimal conditions for the extraction of biologically active compounds (BACs) from medicinal plant raw materials contributes to their exhaustive extraction, correct determination and high degree of conversion of raw materials.

A study of effect of distilled water and 80% ethanol used as extractants was carried out to determine the efficiency of extraction of water-soluble polysaccharides and free carbohydrates contained in medicinal plant raw materials and plantain meal of the large Plantago major L. plantain family Plantaginaceae. The comparison results of the conversion degree of raw materials depending on the use of these extractants are presented.

Extracts were obtained in two ways, the schemes of which are presented in the work. Method 1 - aqueous extract No. 1, obtained directly from raw materials, method 2 – aqueous extract No. 2 obtained from extractive substances after removing 80% of ethanol, initially used as an extractant; aqueous extract No. 3 obtained from meal 2. The content of water-soluble polysaccharides in the extracts was determined by the gravimetric method after their precipitation with 96% ethanol, the total content of free carbohydrates was determined by the spectrophotometric method after reaction with 1% picric acid, having previously determined the maximum wavelength and the optimal reaction time. The conversion degree of plant raw materials was calculated after extracting the indicated BAS in two ways.

According to the experimental data obtained, it was noted that when obtaining aqueous extracts from medicinal plant raw materials of leaves plantain using method 2, characterized by preliminary alcohol extraction, the concentration of the BAS of interest was greater, and the conversion degree of the raw materials was higher by 1.4.

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Author Biography

Tatyana Mikhailovna Grebenkina , Samara Federal Research Center of the Russian Academy of Sciences, Institute of Ecology of the Volga Basin of the Russian Academy of Sciences

candidate of biological sciences, research engineer

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Published
2024-09-25
How to Cite
1. Grebenkina T. M. THE EFFECT OF EXTRACTANTS ON THE EFFICIENCY OF EXTRACTION OF WATER-SOLUBLE POLYSACCHARIDES AND FREE CARBOHYDRATES IN MEDICINAL PLANT RAW MATERIALS OF PLANTAIN // chemistry of plant raw material, 2024. № 3. P. 242-249. URL: http://journal.asu.ru/cw/article/view/13746.
Issue
No 3 (2024)
Section
Low-molecular weight compounds

Copyright (c) 2024 chemistry of plant raw material

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