COMPARATIVE ANALYSIS OF PHENOLIC COMPOUNDS CONTENT IN VACCINIUM CORYMBOSUM L. TISSUES AND CELLS IN IN VIVO AND IN VITRO CONDITIONS

UDC 581.19

DOI_disc_logo https://doi.org/10.14258/jcprm.20240313979
Keywords: Vaccinium corymbosum, leaves, berries, calluses, cell suspension culture, phenolic compounds

Abstract

In leaves, berries, calluses, and suspension cells of highbush blueberry Vaccinium corymbosum L. it was photometrically determined content of phenolic compounds (i.e. soluble phenolic compounds, flavonoids, flavans, and proanthocyanidins). It was shown that highbush blueberry callus and suspension cultures retain the ability to accumulate phenolic compounds, which is comparable to the species leaves and berries. In particular, content of soluble phenolic compounds in leaves is about 270 mg/g dry weight, in berries it is about 70 mg/g dry weight, in calluses it reaches 35–102 mg/g dry weight, and in suspension cultures it reaches 125–150 mg/g dry weight depending on cultures origin (on explant type). In phenolic complex of callus and suspension cultures, as well as in those of leaves, flavonoids predominate (25–146 mg/g dry weight and 180 mg/g dry weight, respectively), and in suspension cultures, initiated from calluses of leaf origin, flavans and proanthocyanidins accumulation increases in comparison with leaves. This study indicates that highbush blueberry suspension cultures, initiated from calluses of leaf origin, may serve as an alternative to plants from greenhouse for production of economically valuable phenolic compounds characteristic of heather plants.

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Author Biographies

Ekaterina Vasilievna Berezina, Lobachevsky State University of Nizhny Novgorod

candidate of biological sciences, associate professor of the department of biochemistry and biotechnology

Dmitry Alekseevich Rybin, Lobachevsky State University of Nizhny Novgorod

PhD student, Department of Biochemistry and Biotechnology, Institute of Biology and Biomedicine

Alina Alekberovna Sukhova, Lobachevsky State University of Nizhny Novgorod

student, Department of Biochemistry and Biotechnology, Institute of Biology and Biomedicine

Andrey Anatolyevich Syomin, Lobachevsky State University of Nizhny Novgorod

student, Department of Biochemistry and Biotechnology, Institute of Biology and Biomedicine

Irina Valentinovna Mishukova, Lobachevsky State University of Nizhny Novgorod

leading biologist, Collection Department, Botanical garden, Institute of Biology and Biomedicine

Anna Aleksandrovna Brilkina, Lobachevsky State University of Nizhny Novgorod

head of the department of biochemistry and biotechnology, candidate of biological sciences

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Published
2024-09-24
How to Cite
1. Berezina E. V., Rybin D. A., Sukhova A. A., Syomin A. A., Mishukova I. V., Brilkina A. A. COMPARATIVE ANALYSIS OF PHENOLIC COMPOUNDS CONTENT IN VACCINIUM CORYMBOSUM L. TISSUES AND CELLS IN IN VIVO AND IN VITRO CONDITIONS // chemistry of plant raw material, 2024. № 3. P. 129-137. URL: http://journal.asu.ru/cw/article/view/13979.
Issue
No 3 (2024)
Section
Low-molecular weight compounds

Copyright (c) 2024 chemistry of plant raw material

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