DETERMINATION OF OPTIMUM CONDITIONS OF EXTRACTION FOR INVESTIGATION OF COMPOSITION OF PHENOLIC COMPOUNDS SPIRAEA BETULIFOLIA PALL. BY HPLC METHOD
Abstract
Studies on optimizing the conditions of extraction of phenolic compounds from the leaves of Spiraea betulifolia Pall. for further chemotaxonomic and phytochemical research by high performance liquid chromatography (HPLC) are conducted. Concentration of extraction agent (water and water – ethanol 20, 40, 50, 60, 70, 80 and 96% extraction) and the mass of raw sample (0,1, 0,2, 0,4, 0,6, 0,8 and 1 g) were selected as variable factors. Qualitative composition of phenolic compounds on HPLC ‒ chromatograms served as indicator of efficiency of extraction. Studies have shown that concentrated 40–70% water-ethanol extracts with the mass of raw sample 0,1 ‒ 1 g are more suitable for the detection of the whole of composition of phenolic compounds present in the leaves of S. betulifolia by HPLC method. Twenty five compounds found in the leaves of S. betulifolia by HPLC method. Nine substances were identified as chlorogenic and n-coumaric acid, dihydroquercetin, quercetin, kaempferol, rutin, giperozid, avikulyarin and astragalin.
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