DEVELOPMENT AND VALIDATION OF THE EXPRESS TECHNIQUE OF ALLOCATION AND QUANTITATIVE DEFINITION OF WATER-SOLUBLE POLYSACCHARIDES OF LEAVES OF ARCTIUM LAPPA L.
Abstract
Research objective was development of an express technique of allocation and quantitative definition of water-soluble polysaccharides in leaves of a Arctium lappa L. For acceleration of process of extraction of biologically active agents of leaves of a Arctium lappa and also augmentations of an exit of water-soluble polysaccharides, it was decided to use an ultrasonic bathtub. Varying process indicators, it was succeeded to pick up extraction conditions under which process of extraction and quantitative definition of water-soluble polysaccharides of leaves of a Arctium lappa comes down to 1.5 h, namely: an izmelchennost of raw materials of 0.5–1.0 mm, temperature – 80 °С, frequency rate of extraction – 3, duration of extractions – 15 min, ultrasound frequency – 35 kHz, a ratio of raw materials and the ekstragent of 1 g for 15 ml. The developed technique of a validirovan on indicators of repeatability, correctness, fastness and linearity therefore it is proved that it is a pretsizionna in the conditions of repeatability, is correct, steady and has rather rigid linear dependence of mass of a deposit on mass of the analyzed raw materials at gravimetric definition of water-soluble polysaccharides in leaves of а Arctium lappa. The technique can be used for the express analysis of quality of leaves of а Arctium lappa and also at industrial receiving an inulin from this type of raw materials.
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Trineeva O.V., Slivkin A.I. Vestnik Voronezhskogo gosudarstvennogo universiteta. Seriia: Khimiia. Biologiia. Far-matsiia, 2017, no. 1, pp. 164–169. (in Russ.).
Velikanova N.A., Gaponov S.P., Slivkin A.I. Ekootsenka lekarstvennogo rastitel'nogo syr'ia v urbousloviiakh g. Voronezha. [Eco-assessment of medicinal plants in the urban environment of Voronezh]. LAMBERT Academic Publishing, 2013, 211 p. (in Russ.).
Iuzepchuk S.V., Sergievskaia E.V. Flora SSSR Botanicheskii institut imeni V.L. Komarova Akademii nauk SSSR. [Flora of the USSR Botanical Institute named after V.L. Komarov Academy of Sciences of the USSR]. Moscow, Leningrad, 1962, vol. XXVII, p. 97. (in Russ.).
Botaniko-farmakognosticheskii slovar': spravochnoe posobie [Botanico-pharmacognostic dictionary: reference book], ed. K.F. Blinova, G.P. Iakovlev. Moscow, 1990, 206 p. (in Russ.).
Kovalev O.A., Fedoseeva L.M., Bindiuk M.A. Institut stomatologii, 2009, vol. 4, no. 45, pp. 74–75. (in Russ.).
Kurkin V.A. Farmakognoziia. [Pharmacognosy]. Samara, 2004, 1180 p. (in Russ.).
Savina A.A. i dr. Khimiko-farmatsevticheskii zhurnal, 2006, vol. 40, no. 11, pp. 43–45. (in Russ.).
Iaseniavskaia A.L. i dr. Farmatsevticheskie nauki: ot teorii k praktike: zaochnaia nauchno-prakticheskaia konferentsiia s mezhdunarodnym uchastiem. [Pharmaceutical Sciences: from theory to practice: extramural scientific-practical conference with international participation]. Astrakhan', 2016, pp. 163–165. (in Russ.).
Bogacheva N.G., Koniaeva E.A., Alent'eva O.G. Voprosy biologicheskoi, meditsinskoi i farmatsevticheskoi khimii, 2015, no. 7, pp. 30–33. (in Russ.).
Knaub N.N. Fitokhimicheskoe issledovanie i perspektivy ispol'zovaniia list'ev lopukha bol'shogo, proizra-staiushchego v Altaiskom krae, v kachestve lekarstvennogo syr'ia: avtoref. dis. … kand. farm. nauk. [Phytochemical study and prospects for the use of burdock leaves, growing in the Altai Territory, as a medicinal raw material: author. dis. ... Candidate of Pharmaceutical Sciences]. Perm', 2006, 23 p. (in Russ.).
Fedoseeva L.M., Knaub N.N., Seligeeva T.G. Khimiia rastitel'nogo syr'ia, 2004, no. 1, pp. 61–64. (in Russ.).
D'iakova N.A. i dr. Vestnik VGU. Seriia: Khimiia, Biologiia, Farmatsiia, 2016, no. 4, pp. 133–136. (in Russ.).
Shushunova T.G. i dr. Puti i formy sovershenstvovaniia farmatsevticheskogo obrazovaniia. Sozdanie novykh fiziolog-icheski aktivnykh veshchestv: materialy 6-i Mezhdunarodnoi nauchno-metodicheskoi konferentsii «Farmobrazovanie-2016». [Ways and forms of improving pharmaceutical education. Creation of new physiologically active substances: materials of the 6th International Scientific and Methodological Conference "Pharmaceutical Education-2016"]. Voro-nezh, 2016, pp. 609–612. (in Russ.).
Fedoseeva L.M., Bindiuk M.A. Khimiia rastitel'nogo syr'ia, 2008, no. 1, pp. 149–150. (in Russ.).
Molchanov G.I., Molchanov A.A., Kubalova L.M. Farmatsevticheskie tekhnologii: sovremennye elektorofizi-cheskie biotekhnologii v farmatsii. [Pharmaceutical technologies: modern electrophysical biotechnology in pharmacy]. Moskva, 2011, 307 p. (in Russ.).
D'iakova N.A. i dr. Sovremennaia farmatsiia: problemy i perspektivy razvitiia materialy V mezhregional'noi na-uchno-prakticheskoi konferentsii s mezhdunarodnym uchastiem. [Modern Pharmacy: Problems and Development Prospects Materials of the V Interregional Scientific and Practical Conference with International Participation]. Vladikavkaz, 2015, pp. 45–48. (in Russ.).
D'iakova N.A. i dr. Vestnik Voronezhskogo gosudarstvennogo universiteta. Seriia: Khimiia. Biologiia. Farmatsiia, 2015, no. 2, pp. 106–111. (in Russ.).
D'iakova N.A. i dr. Khimiko-farmatsevticheskii zhurnal, 2015, vol. 49, no. 9, pp. 35–38. (in Russ.).
Gosudarstvennaia farmakopeia Rossiiskoi Federatsii. XIII izdanie. [State Pharmacopoeia of the Russian Federation. XIII edition]. Moscow, 2015, vol. 1, 1470 p. (in Russ.).
Gavrilin M.V., Senchenko S.P. Validatsiia analiticheskikh metodik (metodicheskie ukazaniia dlia aspirantov i studen-tov). [Validation of analytical methods (guidelines for graduate students and students)]. Piatigorsk, 2008, 37 p. (in Russ.).
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