STUDY OF COMPONENT COMPOSITION OF MEDICINAL PLANT MATERIALS BY GAS CHROMATOGRAPHY WITH MASS-SPECTROMETRIC DETECTION
Abstract
Component composition of extracts 14 medicinal materials kinds was study by gas chromatography with mass-spectrometric detection for revealing of the marker compounds specific for particular plant species. Extraction of components from the investigated objects was carried out by extraction of dry ground raw material with ethanol. For the analysis of polar compounds, the extracts were further derivatized to give the corresponding trimethylsilyl derivatives. Identification of components was carried out using commercial (NIST11, Wiley14) and own custom mass-spectrometer libraries. A number of compounds with species specificity were found for Schisandra chinensis, Ginkgo biloba, Oplopanax elatus, Oplopanax horridus, Ferula soongarica, Epimedium brevicornu, Epimedium koreanu, Cnidium Monnieri, Tribulus terrestris. The possibility of practical application of identified marker compounds for establishing the authenticity and quality control of multicomponent plant gathering and biologically active food additives on the example of the analysis BAA «Vertera Endo mix», «REDTEST», «Devil’s Club» is shown. Based on the analysis of extracts Panax ginseng, Aralia mandshurica, Rhodiola rosea, Eurycoma longifolia and Eleutherococcus senticosus, it was not possible to identify marker compounds
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Rukovodstvo po metodam kontrolya kachestva i bezopasnosti biologicheski aktivnykh dobavok k pishche. R 4.1.1672-03, MinZdrav RF. [Guidance on methods for quality control and safety of biologically active food additives. P 4.1.1672-03, Ministry of Health of the Russian Federation]. Moscow, 2004. (in Russ.).
Metodicheskiye rekomendatsii. «Rekomenduyemyye urovni potrebleniya pishchevykh i biologicheski aktivnykh ve-shchestv», MR 2.3.1. 19150-04. [Methodical recommendations. "Recommended levels of consumption of food and bio-logically active substances", MP 2.3.1. 19150-04]. Moscow, 2004. (in Russ.).
Komarov Ye.L., Vlasov A.M., Eller K.I. Rynok BAD, 2005, no. 5(25), pp. 33–35. (in Russ.).
Gosudarstvennaya farmakopeya Rossiyskoy Federatsii. [State Pharmacopoeia of the Russian Federation]. XIII Edition. In 3 vol. Moscow, 2015. (in Russ.).
Crane P. Ginkgo: the tree that time forgot // New Haven and London: Yale University Press. 2013. 350 p.
Kurkina A.V., Kalabukhova Ye.A., Vlasova G.I., Demidova G.A., Avdeyeva Ye.V. Sovremennyye problemy nauki i obrazovaniya, 2013, no. 4, pp. 352–358. (in Russ.).
Marchenko M.A., Zilfikarov I.N., Ibragimov T.A., Maleyev A.G. Farmakognoziya, botanika, 2017, vol. 5, no. 3, pp. 222–241. DOI: 10.19163/2307-9266-2017-5-3-222-241. (in Russ.).
Razzhivin R.V., Reshetnyak V.Yu., Kuz'menko A.N., Nesterova O.V., Popkov V.A. Vestnik Moskovskogo univer-siteta. Seriya 2. Khimiya, 2009, vol. 50, no. 1, pp. 67–70. (in Russ.).
Tutelyan V.A., Eller K.I., Vlasov A.M. Pitaniye zdorovogo i bol'nogo cheloveka: Materialy III mezhregio-nal'noy nauchno-prakticheskoy konferentsii. [Nutrition for a healthy and sick person: Proceedings of the III Interregional Scien-tific and Practical Conference]. St. Petersburg, 2005, pp. 201–202. (in Russ.).
Eller K.I., Solov'yeva O.I. Biologicheski aktivnyye dobavki k pishche: XXI vek: Tezisy doklada IV Mezhduna-rodnogo simpoziuma. [Biologically active food additives: XXI century: Abstracts of the IV International Symposium]. St. Pe-tersburg, 2000, pp. 598. (in Russ.).
Kuz'menko A.N., Krasnyuk I.I., Pirogov A.V. Vestnik Moskovskogo universiteta. Seriya 2. Khimiya, 2014, vol. 55, no. 4, pp. 214–218. (in Russ.).
Razzhivin R.V., Reshetnyak V.YU., Kuz'menko A.N., Nesterova O.V., Popkov V.A. Vestnik Moskovskogo univer-siteta. Seriya 2. Khimiya, 2009, vol. 50, no. 2, pp. 129–132. (in Russ.).
Khotimchenko S.V., Vas'kovskiy V.Ye. Bioorganicheskaya khimiya, 2004, vol. 30, no. 2, pp. 190–194. (in Russ.).
Dias A.C.P., Tomas-Barberan F.A., Fernandes-Ferreira M., Ferreres F. II Phytochemistry, 1998, vol. 48, no. 7, pp. 1165–1168. DOI: 10.1016/S0031-9422(97)00963-1.
Chadin I. Vestnik Instituta biologii Komi NTS UrO RAN, 2001, issue 46, no. 8, pp. 23–25. (in Russ.).
Metodika izmereniy soderzhaniya ikariina, ikaritina, ikarizida I, ikarizida II, epimedina A, epimedina V i epimedina S v biologicheski aktivnykh dobavkakh metodom vysokoeffektivnoy zhidkostnoy khromatografii s primeneniyem tandemnoy mass-spektrometrii. [Method for measuring the content of Icariin, Ikaritin, Ikarizide I, Ikarizide II, epimedine A, epimedine B and epimedine C in biologically active additives by high-performance liquid chromatography using tandem mass spectrometry]. (№6.00163/2-28-2016 от 28.03.16). (in Russ.).
Kozlova O.I., Perederyayev O.I., Ramenskaya G.V. Voprosy pitaniya, 2011, vol. 8, no. 6, pp. 67–71. (in Russ.).
Dinchev D., Janda B., Evstatieva L., Oleszek W., Aslani M.R., Kostova I. Phytochemistry, 2008, vol. 69, pp. 176–186. DOI: 10.1016/j.phytochem.2007.07.003.
Ivanova A., Lazarova I., Mechkarova P., Tchorbanov B. Biotechnology & Biotechnological Equipment, 2010, vol. 24, no. 1, pp. 129–133. DOI: 10.1080/13102818.2010.10817826.
Powers C.N., Setzer W.N. In Silico Pharmacology, 2015, vol. 3, issue 1, Article 4, pр. 1–63. DOI: 10.1186/s40203-015-0008-z.
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