AMINO-ACID COMPOSITION OF ATRIPLEX PATULA L. AND AMARANTHUS RETROFLEXUS L. (AMARANTHACE-AE) GROWING IN CENTRAL YAKUTIA
Abstract
The research goal is comparative study the amino acid composition of seeds and leaves of Atriplex patula L. and Amaranthus retroflexus L. from the flora of Central Yakutia (the Republic of Sakha (Yakutia). Leaves and seeds of A. patula and A. retroflexus were sampled in August 2016 in the fruiting phase. Biochemical and amino acid composition, the content of crude protein, fat, calcium and phosphorus of the abovementioned plants was studied at the premises of the State budgetary institution of the Republic of Sakha (Yakutia) "Yakut Republican Veterinary Testing Laboratory". From 14 studied amino acids, 9 are stated to be irreplaceable. The total amount of the studied amino acids in A. patula made up 3.3±0.2% in leaves and 3.6±0.2% in seeds; while A. retroflexus contained 4.2±0.2% in leaves and 3.8±0.2% in seeds. The irreplaceable amino acid composition included lysine, leucine and isoleucine, methionine, valine, threonine, arginine, histidine, and phenylalanine. The interchangeable amino acid composition was represented by tyrosine, proline, serine, alanine and glycine. The sum of irreplaceable amino acids in A. patula made up 2.07±0.10% in leaves and 2.30±0.12% in seeds; in A. retroflexus the irreplaceable amino acids totaled 2.63±0.13% in leaves and 2.20±0.11% in seeds. It should be noted that histidine is absent in seeds and the low content of phenylalanine in seeds – 2.8 times and leaves – 4.5 times in A. patula compared to A. retroflexus. The obtained data indicate that in the leaves and seeds of A. retroflexus in comparison with A. patula, the content of crude protein is 1.5 times higher, the calcium content is higher to 2.3 times, the phosphorus content is lower: in leaves 3.3 times, in seeds - in 1.4 times. The results of the study show the biological value and perspectivity of the two studied species as a promising source of natural biological active substances to be used in medicine and agriculture.
Downloads
Metrics
References
Soboleva V.A., Chushenko V.N., Kolomiets A.A. Elektronnyi zhurnal «Provizor», 2010, no. 17. URL: http://www.provisor.com.ua/archive/2010/N17/kashk_1710.php?part_code=40&art_code=7488 (in Russ.).
Simonian A.V., Salamatov A.A., Pokrovskaia Iu.S., Avanesian A.A. Ispol'zovanie ningidrinovoi reaktsii dlia kolich-estvennogo opredeleniia α-aminokislot v razlichnykh ob"ektakh. [Use of ninhydrin reaction for quantitative determina-tion of α-amino acids in various objects]. Volgograd, 2007, 106 p. (in Russ.).
Gubin K.V., Khanina M.A. Meditsina i obrazovanie v Sibiri: elektronnyi nauchnyi zhurnal, 2011, no. 5. URL: http://www.ngmu.ru/cozo/mos/article/text_full.php?id=538. (in Russ.).
Shtarkman I.N. Antioksidantnye svoistva aminokislot i obrazovanie dolgozhivushchikh radikalov belka pod deistviem rentgenovskogo izlucheniia: dis. …kand. biol. nauk. [Antioxidant properties of amino acids and the formation of long-lived protein radicals under the action of X-rays: dis. ... cand. Biol. sciences.]. Pushchino, 2008, 107 p. (in Russ.).
Malyshev L.I., Peshkova G.A., Baikov K.S. i dr. Konspekt flory Sibiri: Sosudistye rasteniia. [Abstract of the flora of Siberia: Vascular plants]. Novosibirsk, 2005, 362 p. (in Russ.).
Edvars Dzh., Uoker D. Fotosintez S3- i S4-rastenii: mekhanizmy i reguliatsiia. [Photosynthesis of C3 and C4 plants: mechanisms and regulation]. Moscow, 1986, 598 p. (in Russ.).
Timofeev N.P. Netraditsionnye prirodnye resursy, innovatsionnye tekhnologii i produkty: sbor-nik nauchnykh trudov. [Non-traditional natural resources, innovative technologies and products: a collection of scientific papers]. Moscow, 2002, vol. 6, pp. 115–139. (in Russ.).
Kislova N.M. Poleznye svoistva sorniakov. [Useful properties of weeds]. Moscow, 2009, 288 p. (in Russ.).
Kononkov P.F., Gins V.K., Gins M.S. Amarant – netraditsionnaia kul'tura. [Amaranth is an unconventional culture]. Moscow, 1997, 160 p. (in Russ.).
Babich A.A., Olonicheva R.V., Prokopenko L.S, Pirin N.I., Bugaiov V.D., Kir'iachenko SP. Vtoroi Mezhdunaronyi simpozium «Novye i netraditsionnye rasteniia i perspektivy ikh prakticheskogo ispol'zovaniia». [The Second Interna-tional Symposium "New and non-traditional plants and prospects for their practical use"]. Moscow, 1997, pp. 35–37. (in Russ.).
Zhuravskaia A.N., Voronov I.V., Poskachina E.R. Vestnik Severo-Vostochnogo federal'nogo universiteta imeni M.K. Ammosova, 2012, no. 2, pp. 47–52. (in Russ.).
GOST R55569-2013. Korma, kombikorma, kombikormovoe syr'e. Opredelenie proteinogennykh aminokislot metodom kapilliarnogo elektroforeza. [GOST R55569-2013. Forage, mixed fodder, feed forage. Determination of proteinogenic amino acids by the method of capillary electrophoresis]. Moscow, 2014, 18 p. (in Russ.).
GOST R51417-99. Korma, kombikorma, kombikormovoe syr'e. Opredelenie massovoi doli azota i vychisle-nie masso-voi doli syrogo proteina, metod K"el'dalia. [GOST R51417-99. Forage, mixed fodder, feed forage. Determination of the mass fraction of nitrogen and calculation of the mass fraction of crude protein, the Kjeldahl method.]. Moscow, 2002, 8 p. (in Russ.).
GOST 13496.15-97. Korma, kombikorma, kombikormovoe syr'e. Metody opredeleniia soderzhaniia syrogo zhira. [Forage, mixed fodder, feed forage. Methods for determining the content of raw fat]. Moscow, 2011, 12 p. (in Russ.).
GOST 32904-2014. Korma, kombikorma. Opredelenie soderzhaniia kal'tsiia titrimetricheskim metodom. [GOST 32904-2014. Forage, mixed fodder. Determination of calcium content by titrimetric method]. Moscow, 2016, 10 p. (in Russ.).
GOST 26657-97. Korma, kombikorma, kombikormovoe syr'e. Metody opredeleniia soderzhaniia fosfora. [GOST 26657-97. Forage, mixed fodder, feed forage. Methods for determination of phosphorus content]. Minsk, 1999, 12 p. (in Russ.).
Kalamaki M.S., Alexandrou D., Lazari D., Merkouropoulos G., Fotopoulos V., Pateraki I., Aggelis A., Carrillo-López A., Rubio-Cabetas M.J., Kanellis A.K. J. Exp. Bot., 2009, vol. 60, no. 6, pp. 1859–1871.
Todorov I.N., Todorov G.N. Stress, starenie i ikh biokhimicheskaia korrektsiia. [Stress, aging and their biochemical correction]. Moscow, 2003, 479 p. (in Russ.).
Starr C., McMillan B. Human Biology. 11 ed. Cengage Learning, 2014, p. 16.
Zhivoedov P.M., Zhirov V.K., Rudenko S.M. Belkovyi sostav i membrannye lipidy introdutsirovannykh rastenii v Zapoliar'e. [Protein composition and membrane lipids of introduced plants in the Arctic]. Apatity, 1987, 113 p. (in Russ.).
Petrov K.A. Kriorezistentnost' rastenii: ekologo-fiziologicheskie i biokhimicheskie aspekty. [Cryopresistance of plants: ecology-physiological and biochemical aspects]. Novosibirsk, 2016, 276 p. (in Russ.).
Soshnikova O.V., Iatsiuk V.Ia. Rossiiskii Mediko-biologicheskii vestnik im. akademika I.P. Pavlova, 2010, no. 2, pp. 135–141. (in Russ.).
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
