THE INFLUENCE OF CULTIVATION CONDITIONS ON MORPHOPHYSIOLOGICAL ACTIVITY AND CONTENT OF PHENOLIC COMPOUNDS OF ST. JOHN'S WORT (HYPERICUM PERFORATUM L.) IN VITRO CULTURE

  • Вера (Vera) Николаевна (Nikolaevna) Овчинникова (Ovchinnikova) All-Russian Scientific Research Institute of Agricultural Biotechnology Email: vera.ovchinnikova.1957@mail.ru
  • Наталья (Natal'ya) Петровна (Petrovna) Карсункина (Karsunkina) Russian State Agrarian University named after K.A. Timiriazev Email: vera.ovchinnikova.1957@mail.ru
  • Петр (Petr) Николаевич (Nikolaevich) Харченко (Kharchenko) All-Russian Scientific Research Institute of Agricultural Biotechnology Email: kharchenko@iab.ac.ru
  • Наталья (Natal'ya) Владимировна (Vladimirovna) Никифорова (Nikiforova) All-Russian Scientific Research Institute of Agricultural Biotechnology Email: nikif_natali@mail.ru
Keywords: in vitro cultivation, St. John's wort (Hypericum perforatum L.), LEDs, cytokinins, the total content of soluble phenolic compounds (TCSPC)

Abstract

The paper discusses the joint effect of cytokine 6-BAP and different spectra when illuminated by LEDs on the morphological parameters of growth, development and the content of phenolic compounds in plants of St. John's Wort (Hypericum perforatum L.) of two genotypes – wild and cultivated (cultivar Zolotodolinskiy) – in conditions of in vitro cultivation.

It is shown that the light of different spectral composition and the hormonal composition of medium influences the morphogenesis, the productivity of biomass and the synthesis of phenolic compounds by plants under in vitro cultivation conditions.

It is established that the combination of the light spectrum, and the hormonal composition of nutrient medium may substantially increase the content of soluble phenolic compounds in both wild and cultural genotypes of Hypericum.

The analysis of the obtained results shows a direct correlation dependence of the content of phenolic compounds on the number of shoots and their mass. The correlation between the number of shoots and the content of phenolic compounds has a direct character and strongly expressed.

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Author Biographies

Вера (Vera) Николаевна (Nikolaevna) Овчинникова (Ovchinnikova), All-Russian Scientific Research Institute of Agricultural Biotechnology
ведущий научный сотрудник, кандидат биологических наук
Наталья (Natal'ya) Петровна (Petrovna) Карсункина (Karsunkina), Russian State Agrarian University named after K.A. Timiriazev
доцент, кандидат биологических наук
Петр (Petr) Николаевич (Nikolaevich) Харченко (Kharchenko), All-Russian Scientific Research Institute of Agricultural Biotechnology
научный руководитель института, академик РАН
Наталья (Natal'ya) Владимировна (Vladimirovna) Никифорова (Nikiforova), All-Russian Scientific Research Institute of Agricultural Biotechnology
младший научный сотрудник

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Published
2018-03-19
How to Cite
1. Овчинникова (Ovchinnikova)В. (Vera) Н. (Nikolaevna), Карсункина (Karsunkina)Н. (Natal’ya) П. (Petrovna), Харченко (Kharchenko)П. (Petr) Н. (Nikolaevich), Никифорова (Nikiforova)Н. (Natal’ya) В. (Vladimirovna) THE INFLUENCE OF CULTIVATION CONDITIONS ON MORPHOPHYSIOLOGICAL ACTIVITY AND CONTENT OF PHENOLIC COMPOUNDS OF ST. JOHN’S WORT (HYPERICUM PERFORATUM L.) IN VITRO CULTURE // chemistry of plant raw material, 2018. № 3. P. 223-229. URL: http://journal.asu.ru/cw/article/view/3747.
Section
Biotechnology