DEVELOPMENT AND VALIDATION OF IDENTIFICATION METHOD OF CALENDULOSIDES IN THE MARIGOLD TINCTURE. MESSAGE 2.

  • Екатерина (Ekaterina) Александровна (Aleksandrovna) Хохлова (Khokhlova) National University of Pharmacy, Pushkinskaya str., 53, Kharkov, 61002 Email: kateryna_khokhlova@ukr.net
  • Александр (Aleksandr) Анатольевич (Anatol'evich) Здорик (Zdorik) National University of Pharmacy, Pushkinskaya str., 53, Kharkov, 61002 Email: oleksandr_zdoryk@ukr.net
  • Виктория (Viktorija) Акоповна (Akopovna) Георгиянц (Georgijanc) National University of Pharmacy, Pushkinskaya str., 53, Kharkov, 61002 Email: vgeor@ukr.net
  • Лилия (Lilija) Ивановна (Ivanovna) Вишневская (Vishnevskaja) National University of Pharmacy, Pushkinskaya str., 53, Kharkov, 61002 Email: lilya-vishn@rambler.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.201502398
Keywords: Marigold tincture, calendulosides, thin-layer chromatography, validation

Abstract

The thin-layer chromatography method of calendulosides’ identification in the Marigold tincture was developed. The validation characteristics such as specificity, robustness, precision were studied. During robustness study the influences of chamber’s type, stationary phase, development distance, conditions of dryness, stability of the test solution during analysis were investigated. The optimal conditions of chromatography analysis were investigated. The specificity of method of calendulosides’ determination was proved with the reference standard of calendulosides. The precision of the method on the same and on the different plates, the interlaboratory precision were studied. The possibility of usage developed method for standardization of Marigold tincture in the condition of the quality control lab was proved. For assuring specificity of identification of Marigold tincture, the determination of characteristic substances for Marigold flower – flavonoids, is reasonable.

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Author Biographies

Екатерина (Ekaterina) Александровна (Aleksandrovna) Хохлова (Khokhlova), National University of Pharmacy, Pushkinskaya str., 53, Kharkov, 61002
ассистент кафедры аптечной технологии лекарств им. Д.П. Сала, кандидат фармацевтических наук, тел. +38(057) 706-35-81
Александр (Aleksandr) Анатольевич (Anatol'evich) Здорик (Zdorik), National University of Pharmacy, Pushkinskaya str., 53, Kharkov, 61002
доцент кафедры фармацевтической химии, кандидат фармацевтических наук
Виктория (Viktorija) Акоповна (Akopovna) Георгиянц (Georgijanc), National University of Pharmacy, Pushkinskaya str., 53, Kharkov, 61002
заведующая кафедрой фармацевтической химии, доктор фармацевтических наук, профессор
Лилия (Lilija) Ивановна (Ivanovna) Вишневская (Vishnevskaja), National University of Pharmacy, Pushkinskaya str., 53, Kharkov, 61002
заведующая кафедрой аптечной технологии лекарств им. Д.П. Сала, доктор фармацевтических наук, профессор

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Published
2015-01-27
How to Cite
1. Хохлова (Khokhlova)Е. (Ekaterina) А. (Aleksandrovna), Здорик (Zdorik)А. (Aleksandr) А. (Anatol’evich), Георгиянц (Georgijanc)В. (Viktorija) А. (Akopovna), Вишневская (Vishnevskaja)Л. (Lilija) И. (Ivanovna) DEVELOPMENT AND VALIDATION OF IDENTIFICATION METHOD OF CALENDULOSIDES IN THE MARIGOLD TINCTURE. MESSAGE 2. // chemistry of plant raw material, 2015. № 2. P. 195-199. URL: http://journal.asu.ru/cw/article/view/398.
Issue
No 2 (2015)
Section
Low-molecular weight compounds

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