THE EFFECT OF NANOSCALE SELENIUM ON THE CAUSATIVE AGENT OF RING ROT AND POTATO IN VITRO
Abstract
The biological activity of biopolymer-based selenium nanocomposite with a high selenium content of 6.4% was investigated. It was shown earlier that nanocomposites of selenium and arabinogalactan (NC Se/AG, 1.23% and 3.4% Se) have a bactericidal and bacteriostatic effect on the pathogenic bacterium Clavibacter michiganensis subsp. sepedonicus (Cms) and do not adversely affect the vitality of potato plants in vitro. In this work, it was shown that the nanocomposite under scrutiny has a bacteriostatic effect, inhibits bacterial growth by 20% compared with the check variant and reduces the ability of Cms to form biofilms, which contribute to their high resistance to external factors. In the experiments conducted on plants, it was shown, there was not negative effect of NC Se/AG on biometric parameters, furthermore, this NC reduces the negative effect of potato infection with Cms. The nanocomposite reduced the activity of peroxidase and the content of reactive oxygen species in potato tissues. It was established that selenium does not accumulate in potato plants after the treatment with NC Se/AG. The results obtained allow us to consider NC Se/AG (6.4% Se) as an agent for healing cultivated plants from pathogenic bacteria.
Downloads
Metrics
References
Anisimov B.V., Belov G.L., Varitsev YU.A., Yelanskiy S.N., Zhuromskiy G.K., Zavriyev S.K., Zeyruk V.N., Ivanyuk V.G., Kuznetsova M.A., Plyakhnevich M.P., Pshechenkov K.A., Simakov Ye.A., Sklyarova N.P., Stashevski Z., Us-kov A.I., Yashina I.M. Zashchita kartofelya ot bolezney, vrediteley i sornyakov. [Protecting potatoes from diseases, pests and weeds.]. Moscow, 2009. 272 p. (in Russ.).
Eichenlaub R., Gartemann K.H. Annual Review of Phytopathology, 2011, vol. 49, pp. 445–464, DOI: 10.1146/annurev-phyto-072910-095258.
Van der Wolf J.M., Elphinstone J.G., Stead D.E., Metzler M., Muller P., Hukkanen A., Karjalainen R. Epidemiology of Clavibacter michiganensis subsp. sepedonicus in relation to control of bacterial ring rot. Wageningen: Plant Research International B.V., 2005, report 95.
Tran P.A., Webster T. International Journal of Nanomedicine, 2011, vol. 6, pp. 1553–1558, DOI: 10.2147/IJN.S21729.
Fadeyeva T.V., Shurygina I.A., Sukhov B.G., Rai M.K., Shurygin M.G., Umanets V.A., Lesnichaya M.V., Kon'kova T.V., Shurygin D.M. Izvestiya RAN. Seriya fizicheskaya, 2015, vol. 79, no. 2, pp. 297–299, DOI: 10.7868/S036767651502009X. (in Russ.).
Yang X., Yang M., Pang B., Vara M., Xia Y. Chemical Reviews, 2015, vol. 115, no. 19, pp. 10410−10488, DOI: 10.1021/acs.chemrev.5b00193.
Kolesnikova L.I., Karpova Ye.A., Vlasov B.Ya., Sukhov B.G., Trofimov B.A. Byullyuten' eksperimental'noy biologii i meditsiny, 2015, vol. 159, no. 2, pp. 183–187, DOI: 10.1007/s10517-015-2928-3. (in Russ.).
Perfil'yeva A.I., Nozhkina O.A., Graskova I.A., Sidorov A.V., Lesnichaya M.V., Aleksandrova G.P., Dolmaa G., Klimenkov I.V., Sukhov B.G. Izvestiya Akademii nauk. Seriya khimicheskaya, 2018, no. 1, pp. 157–163, DOI: 10.31255/978-5-94797-319-8-626-629. (in Russ.).
Perfileva A.I., Zhivet’yev М.А., Gasisova A.V., Borovskii G.B., Graskova I.А., Sukhov B.G., Trofimov B.А. Journal of Stress Physiology & Biochemistry, 2016, vol. 12, no. 2, pp. 11–16.
Papkina A.V., Perfil'yeva A.I., Zhivet'yev M.A., Borovskiy G.B., Graskova I.A., Lesnichaya M.V., Klimenkov I.V., Sukhov B.G., Trofimov B.A. Doklady akademii nauk, 2015, vol. 461, no. 2, pp. 239–241, DOI: 10.7868/S0869565215030305. (in Russ.).
Papkina A.V., Perfileva A.I., Zhivet’yev M.A., Borovskii G.B., Graskova I.A., Klimenkov I.V., Lesnichaya M.V., Su-khov B.G., Trofimov B.A. Nanotechnologies in Russia, 2015, vol. 10, no. 5–6, pp. 484–491, DOI: 10.1134/S1995078015030131.
Perfileva A.I., Moty’leva S.M., Klimenkov I.V., Graskova I.A., Skhov B.G., Trofimov B.A. Nanotechnologies in Rus-sia, 2017, vol. 12, no. 9–10, pp. 553–558, DOI: 10.1134/S1995078017050093.
Romanenko A.S., Riffel A.A., Graskova I.A., Rachenko M.A. Journal of Phytopatholog, 1999, vol. 147, no. 11–12, pp. 679-686, DOI: 10.1046/j.1439-0434.1999.00450.x.
Roozen N.J.M., van Vuurde J.W.L. Journal of Plant Pathology, 1991, vol. 97, no. 5, pp. 321–334.
Florack D.E., Visser B., de Vries P.M., van Vuurde J.W.L., Stiekema W.J. Netherlands Journal of Plant Pathology, 1993, vol. 99, no. 5–6, pp. 259–268.
Shaginyan I.A., Danilina G.A., Chernukha M.YU., Alekseyeva G.V., Batov A.B. Zhurnal mikrobiologii, epidemio-logii i immunobiologii, 2007, no. 1, pp. 3–8. (in Russ.).
Patent 2557992 (RU). 2015. (in Russ.).
Shurygina I.A., Rodionova L.V., Shurygin M.G., Sukhov B.G., Kuznetsov S.V., Popova L.G., Dremina N.N. Iz-vestiya RAN. Seriya fizicheskaya, 2015, vol. 79, no. 2, pp. 280–282, DOI: 10.7868/S0367676515020271. (in Russ.).
Rodionova L.V., Shurygina I.A., Sukhov B.G., Popova L.G., Shurygin M.G., Artem'yev A.V., Pogodayeva N.N., Kuznetsov S.V., Gusarova N.K., Trofimov B.A. Zhurnal obshchey khimii, 2015, vol. 85, no. 2, pp. 314–316, DOI: 10.1134/S1070363215020218. (in Russ.).
Boyarkin A.N. Biokhimiya, 1951, vol. 16, pp. 352–355. (in Russ.).
Bogino P.C., Oliva M. de las M., Sorroche F.G., Giordano W. International Journal of Molecular Sciences, 2013, vol. 30, no. 14(8), pp. 15838–15859, DOI: 10.3390/ijms140815838.
Rakhmatulina M.R., Nechayeva I.A. Vestnik dermatologii i venerologii, 2015, no. 2, pp. 58–62, DOI: 10.25208/0042-4609-2015-0-2-58-62. (in Russ.).
Okulich V.K., Kabanova A.A., Plotnikov F.V. Mikrobnyye bioplenki v klinicheskoy mikrobiologii i antibakterial'noy terapii. [Microbial biofilms in clinical microbiology and antibiotic therapy]. Vitebsk, 2017, 300 p. (in Russ.).
Velmourougane K., Prasanna R., Saxena A.K. Journal of Basic Microbiology, 2017, vol. 57, no. 7, pp. 548–573, DOI: 10.1002/jobm.201700046.
Danhorn T., Fuqua C. Annual Review of Microbiology, 2007, vol. 61, pp. 401–422, DOI:10.1146/annurev.micro.61.080706.093316.
Dubrovina V.I., Medvedeva S.A., Vityazeva S.A., Kolesnikova O.B., Aleksandrova G.P., Gutsol L.O., Grishchen-ko L.A., Chetveryakova T.D. Struktura i immunomoduliruyushcheye deystviye arabinogalaktana listvennitsy sibirskoy i yego metalloproizvodnykh. [Structure and immunomodulatory effect of arabinogalactan of Siberian larch and its metal derivatives]. Irkutsk, 2007, 145 p. (in Russ.).
Suzuki N., Koussevitzky S., Mittler R., Miller G. Plant Cell. And Environment, 2012, vol. 35, no. 2, pp. 259–270, DOI: 10.1111/j.1365-3040.2011.02336.x.
Lehmann S., Serrano M., L'Haridon F., Tjamos S.E., Metraux J.P. Phytochemistry, 2015, vol. 112, pp. 54–62, DOI: 10.1016/j.phytochem.2014.08.027.
Choudhury F.K., Rivero R.M., Blumwald E., Mittler R. Plant Journal, 2017, vol. 90, no. 5, pp. 856–867, DOI: 10.1111/tpj.13299.
Liebthal M., Dietz K.J. Methods in Molecular Biology, 2017, vol. 1631, pp. 23–39, DOI: 10.1007/978-1-4939-7136-7_2.
Copyright (c) 2019 Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
