ECDYSTEROIDS OF SILENE ITALICA: GLYCOSIDIC AND NONGLYCOSIDIC COMPONENTS AND HPLC-DAD-ESI-MS PROFILE

UDC 582.669.26 : 547.92

  • Daniil Nikolayevich Olennikov Institute of General and Experimental Biology SB RAS http://orcid.org/0000-0001-8194-1061 Email: olennikovdn@mail.ru
  • Nina Igorevna Kashchenko Institute of General and Experimental Biology SB RAS Email: ninkk@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.2019045109
Keywords: Silene italic, Caryophyllaceae, ecdysteroids, HPLC, mass-spectrometry

Abstract

 

Silene italica (L.) Pers. is a species of Caryophyllaceae family which introduced samples contain ecdysteroids that was previously shown (Meng et al., 2001). In this paper, the composition of ecdysteroids of wild-growing S. italica was shown. Using high performance chromatography with diode-array detection and electrospray ionization mass-spectrometry detection (HPLC-DAD-ESI-MS) twenty-two compounds assigned to the ecdysteroid group. The non-glycoside components of S. italica were included twelve compounds and integristerone A, 26-hydroxyintegristerone A, 22-deoxyintegristerone A, 2-deoxyintegristerone A, and 2-deoxypolypodine B were identified for the first time. Ten compounds were characterized as ecdysteroid monoglycosides containing hexose fragment as a carbohydrate part. Trer were derivatives of 20-hydroxyecdysone, polypodine B, ecdysone and 2-deoxypolypodine B. For the first time, the presence of 22-deoxyintegristerone A hexoside non-detected in plant objects was found. Comparative analysis of the ecdysteroids composition of S. italica and well studied species S. italica spp. nemoralis (Báthori et al., 2000, 2002, 2004; Pongrácz et al., 2003; Simon et al., 2002) indicates their proximity. Quantitative analysis of five ecdysteroid content in the organs of S. italica showed that they were unevenly distributed in the plant. The concentration of 20-hydroxyecdysone was 0.10–32.12 mg/g and the total ecdysteroids content was 0.10–40.92 mg/g of dry plant weight. In general, it should be noted that the ability to produce and accumulate ecdysteroids was also observed for the wild samples of S. italica, which cultural samples was noted as ecdysteroid concentrator.

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Author Biographies

Daniil Nikolayevich Olennikov, Institute of General and Experimental Biology SB RAS

доктор фармацевтических наук, ведущий научный сотрудник  лаборатории медико-биологических исследований

Nina Igorevna Kashchenko, Institute of General and Experimental Biology SB RAS

кандидат фармацевтических наук, научный сотрудник лаборатории медико-биологических исследований

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Published
2019-12-27
How to Cite
1. Olennikov D. N., Kashchenko N. I. ECDYSTEROIDS OF SILENE ITALICA: GLYCOSIDIC AND NONGLYCOSIDIC COMPONENTS AND HPLC-DAD-ESI-MS PROFILE // chemistry of plant raw material, 2019. № 4. P. 135-147. URL: http://journal.asu.ru/cw/article/view/5109.
Issue
No 4 (2019)
Section
Low-molecular weight compounds

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