CHARACTERISTICS OF HORNY ALTAI PEATS AND THEIR BIOLOGICAL ACTIVITY IN PLANT TISSUE CULTURE
Abstract
The search for new raw materials of biologically active substances of natural origin is an urgent task for the modern period. Peat in this respect is a relatively cheap and almost unlimited raw material base. Peat of swamps can be used widely in agriculture for receipt of biologically active substances are of great interest in the territory of the Gorny Altai. The purpose of this work is to study the composition of organic matter of peats of the Gorny Altai, to choose peat raw materials for biologically active production and to study their biological activity. The object of the study was 46 swamps of the Gorny Altai. The following analyses were carried out in peats: botanical composition, degree of decomposition, ash content, group composition of peat organic matter. The composition of humic acids (ha) was analyzed by IR spectroscopy. For determine of the biological activity of humic acids was used plant tissue cultured . The results of the research allowed to distinguish peats by the content of HA: buckbean peat (47.0% of HA), wood peat (50.0% of HA), fern peat (55.0% of HA), grass peat (30.0–45.0% of HA), sedge peat (5.6–58.0% of HA), grass-buckbean peat (43.0–56.5% of HA) and to outline the raw material base for the production of BAS – peatland Turochak. According to the optimal characteristics of HAs, a sample was taken from a depth of 325-375 cm, HA was isolated. The biological activity In humic acid was determined with the use of plant tissue culture. High biological activity are proven of the preparations of HA from the peatland Turochak, which resulted in the acceleration of microclonal propagation of plants in vitro.
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Tomson A.E. Naumova G.V. Torf i produkty yego pererabotki. [Peat and its processed products]. Minsk, 2009, 328 p. (in Russ.).
Popov A.I. Guminovyye veshchestva – svoystva, stroyeniye, obrazovaniye. [Humic substances – properties, structure, formation]. St. Petersburg, 2004, 212 p. (in Russ.).
Komissarov I.D., Loginov L.F. Guminovyye preparaty. [Humic preparations]. Tyumen, 1971, 266 p. (in Russ.).
Yakimenko O.S., Terekhova V.A. Eurasian Soil Science, 2011, vol. 44, no. 11, pp. 1222–1230. DOI: 10.1134/S1064229311090183.
Thorpe T. Journal of Molecular Microbiology and Biotechnology, 2007, no. 37, pp. 169–180. DOI: 10.1007/s12033-007-0031-3.
Akin-Idowu P.E, Ibitoye D.O., Ademoyegun O.T. African Journal of Biotechnology, 2009, no. 8(16), pp. 3782–3788. DOI: 10.4314/ajb.v8i16.62060.
Garcia-Gonzales R., Quiroz K., Carrasco B., Caligari P. Ciencia e investigación agrarian, 2010, no. 37(3), pp. 5–30. DOI: 10.4067/S0718-16202010000300001.
Mohan N., Nikdad S., Singh G. Biotechnology and Bioengineering, 2011, no. 1(2), pp. 187–194.
Ahmadi A., Azadfar D., Mofidabadi A. International Journal of Plant Production, 2010, no. 4(2), pp. 143–147. DOI: 10.22069/ijpp.2012.690.
Chung S.M., Manjusha V., Tzfira T. Trends in Plant Science, 2006, no. 11(1), pp. 1–4. DOI: 10.1016/j.tplants.2005.11.001.
Purkayastha J., Sugla T., Paul A., Maumdar P., Basu A., Solleti S.K., Mohommad A., Ahmed Z., Sahoo L. Biologia Plantarum, 2010, np. 54, pp. 13–20. DOI: 10.1007/s10535-010-0003-5.
Khan R.S., Alam S.S., Munir I., Azadi P., Nakamura I., Mii M. Plant Cell, Tissue and Organ Culture, 2011, no. 106, pp. 11–20. DOI: 10.1007/s11240-010-9888-0.
Shalaka D.K., Sandhya P. Pharma magazine, 2009, no. 5, pp. 539–363. DOI: 10.4103/0973-1296.58567.
Umamaheswai A., Lalitha V. Journal of Plant Sciences, 2007, no. 2, pp. 545–551. DOI: 10.3923/jps.2007.545.551.
Tyagi R.K., Agrawal A., Mahalakshmi C., Hussain Z., Tyagi H. In Vitro Cellular & Developmental Biology – Plant, 2007, no. 43, pp. 51–58. DOI: 10.1007/s11627-006-9000-y.
Harding K. Aspac Journal of Molecular and Biotechnology, 2010, no. 18(1), pp. 151–154.
Gostischeva M.V., Inisheva L.I. Natural and Nanomaterials in Medicine and Biomedical Technologies: First Interna-tional Conference of Humics-based Innovative Technologies, Moscow, 2010, pp. 16.
Golubkina N.A., Zamana S.P., Tareyeva M.M., Mukhortov V.YU., Pivovarov V.F. Sel'skokhozyaystvennaya biologiya, 2010, no. 2, pp. 41–45. (in Russ.).
Inisheva L.I., Vinogradov V. YU., Golubina O. A., Larina G. V., Porokhina Ye.V., Shinkeyeva N.A., Shurova M.V. Bolotnyye statsionary Tomskogo gosudarstvennogo pedagogicheskogo universiteta. [Swamp hospitals of Tomsk State Pedagogical University]. Tomsk, 2010, 147 p. (in Russ.).
GOST 28245.2-89. Metody opredeleniya botanicheskogo sostava i stepeni razlozheniya. [State standard 28245.2-89. Methods for determining the botanical composition and degree of decomposition]. Moscow, 1989, 5 p. (in Russ.).
GOST 11306-83. Torf i produkty yego pererabotki. [State standard 11306-83. Peat and its products]. Moscow, 1995, 8 p. (in Russ.).
Bazin Ye.T., Kopenkin V.D., Kosov V.I., Korchukov S.S., Petrovich V.M. Tekhnicheskiy analiz torfa. [Technical analy-sis of peat]. Moscow, 1992, 431 p. (in Russ.).
Rozhanskaya O.A., Korolev K.G., Yudina N.V., Lomovskiy O.I. Doklady Rossel'khozakademii, 2008, no. 3, pp. 16–19. (in Russ.).
Rozhanskaya O.A., Inisheva L.I., Stroyeva N.S., Shilova T.V. Vestnik TGPU, 2010, no. 3, pp. 128–130. (in Russ.).
Gamborg O.L., Miller R.A., Ojima K. Experimental Cell Research, 1968, no. 50, pp. 151–158.
Sorokin O.D. Prikladnaya statistika na komp'yutere. [Applied statistics on the computer]. Krasnoobsk, 2009, 222 p. (in Russ.).
Gostishcheva M.V., Belousov M.V., Yusubov M.S., Ismatova R.R., Dmitruk S.Ye. Khimiko-farmatsevticheskiy zhur-nal, 2009, vol. 43, no. 7, pp. 44–47. (in Russ.).
Inisheva L.I., Shaydak L. Sibirskiy vestnik sel'skokhozyaystvennoy nauki, 2015, no. 2, pp. 5–12. (in Russ.).
Orlov D.S. Gumusovyye kisloty pochv i obshchaya teoriya gumifikatsii. [Soil humic acids and the general theory of humi-fication]. Moscow, 1990, 332 p. (in Russ.).
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