DETERMINATION OF HYDROXYCINNAMIC ACIDS, CAROTENOIDS AND CHLOROPHYLL IN THE LEAVES OF STINGING NETTLE (URTICA DIOICA L.)

Abstract

In 2014, it approved a new pharmacopoeia articles for inclusion in the State Pharmacopoeia XIII ed. «Nettle leaves», which, by analogy with the European Pharmacopoeia provides quantification of hydroxycinnamic acids in terms of chlorogenic acid. The value for nettle leaves are biologically active substances such as vitamin K, flavonoids, hydroxycinnamic acids, carotenoids, tannins, amino acids, and other chlorophylls. Nettle leaves are rich in carotenoids, the absorption maximum of which is clearly seen in the spectra of the extracts. It was based on the previously developed method for the quantitative determination of hydroxycinnamic acids and chlorophyll in the leaves of nettle by spectrophotometry. However, the form of the absorption spectrum of the analyte extracting raw indicates the possibility of simultaneous determination of not only the data of biologically active substances, but also without prior separation of carotenoids, because their peaks do not overlap. The work carried out modification techniques quantitative content of hydroxycinnamic acids and chlorophyll in the leaves of nettle by spectrophotometry, which consists in determining the amount of additional carotenoids in terms violoksantin when present together. The influence of the polarity of the extractant to extract the studied groups of biologically active substances. It was found that with increasing polarity to 6.0 units is an increase in extractable all studied groups of substances. Established validation characteristics quantifying carotenoids on the proposed methodology. A comparative analysis of the obtained results with published data to identify the studied groups of biologically active substances of different kinds of plants of the genus Urtica ssp.