POLYSACCHARIDES OF THREE SAUSSUREA DC SPECIES (S. CONTROVERSA, S. SALICIFOLIA, S. FROLOVII): ISOLATION, CHARACTERIZATION AND INFLUENCE ON NO-PRODUCING PROPERTIES OF MACROPHAGES

UDC 615.275.4

  • Yaroslav Yevgen'yevich Reshetov Siberian State Medical University; National Research Tomsk Polytechnic University Email: ferroplex2013@yandex.ru
  • Anastasiya Aleksandrovna Ligacheva Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences Email: vittelli@mail.ru
  • Yelena Yur'yevna Avdeeva Siberian State Medical University Email: elenaavdeev@yandex.ru
  • Marina Grigor'yevna Danilets Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences Email: m.danilets@mail.ru
  • Viktoriya Vladimirovna Golovchenko Institute of Physiology, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences Email: lemnan@mail.ru
  • Yevgeniya Sergeyevna Trofimova Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences Email: eugenie76@mail.ru
  • Yekaterina Igorevna Gulina Siberian State Medical University Email: nevestacherta@mai.ru
  • Yevgeniy Yur'yevich Sherstoboev Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences Email: sherstoboev_eu@pharmso.ru
  • Artem Mikhaylovich Gur'yev Siberian State Medical University; National Research Tomsk Polytechnic University Email: titan-m@mail.ru
  • Kseniya Igorevna Rovkina Siberian State Medical University; National Research Tomsk Polytechnic University Email: rki91@bk.ru
  • Sergey Vladimirovich Krivoshchekov Siberian State Medical University Email: ksv_tsu@mail.ru
  • Mikhail Valer'yevich Belousov Siberian State Medical University; National Research Tomsk Polytechnic University Email: mvb63@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.2019045483
Keywords: Asteraceae, Saussurea DC, polysaccharides, monosaccharide composition, molecular weight, endotoxin, macrophages, nitric oxide

Abstract

Polysaccharides PS1 and PS2 with a yield 1–2% were obtained from the above-ground part of three species of Saussurea: S. controversa DC., S. salicifolia L. and S. frolovii Ledeb. sequential extraction with water at 25 and 70 °C. PS1 free from protein impurities. A protein that is not removed by the Sevag method is co-extracted with PS2. All polysaccharides contain residues of uronic acids, the highest content found in PS1 and PS2 from S. contrоversa. The molecular weights (Mw) of PS1 from S. controversa, S. salicifolia and S. frolovii were 448.13, 158.49, 64.03 kDa and PS2 – 101.82, 94.60, 225.42 kDa, respectively. Interspecific differences in the monosaccharide composition of polysaccharides were revealed. Galactose (Gal) and Arabinose (Ara) residues are major, and Rhamnose (Rha), Xylose (Xyl) and Mannose (Man) residues are minor components of the carbohydrate chains isolated by PS. PS1S. salicifolia and S. frolovii and PS2S. salicifolia do not contain endotoxins impurities and have a NO-activating effect on antigen-presenting cells (macrophages), significantly exceeding the effect of muramyl dipeptide.

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Author Biographies

Yaroslav Yevgen'yevich Reshetov, Siberian State Medical University; National Research Tomsk Polytechnic University

аспирант

Anastasiya Aleksandrovna Ligacheva, Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences

научный сотрудник, кандидат биологических наук

Yelena Yur'yevna Avdeeva, Siberian State Medical University

доцент кафедры фармацевтического анализа, кандидат фармацевтических наук

Marina Grigor'yevna Danilets, Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences

заведующий отделом экспериментальных биологических моделей, доктор биологических наук

Viktoriya Vladimirovna Golovchenko, Institute of Physiology, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences

главный научный сотрудник, доктор химических наук, доцент

Yevgeniya Sergeyevna Trofimova, Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences

старший научный сотрудник, кандидат медицинских наук

Yekaterina Igorevna Gulina, Siberian State Medical University

аспирант

Yevgeniy Yur'yevich Sherstoboev, Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg, Tomsk National Research Medical Center of the Russian Academy of Sciences

заведующий отделом иммунофармакологии, доктор медицинских наук, профессор

Artem Mikhaylovich Gur'yev, Siberian State Medical University; National Research Tomsk Polytechnic University

руководитель центра внедрения технологий, доктор фармацевтических наук

Kseniya Igorevna Rovkina, Siberian State Medical University; National Research Tomsk Polytechnic University

старший преподаватель кафедры химии

Sergey Vladimirovich Krivoshchekov, Siberian State Medical University

младший научный сотрудник, кандидат химических наук

Mikhail Valer'yevich Belousov, Siberian State Medical University; National Research Tomsk Polytechnic University

заведующий кафедрой фармацевтического анализа, доктор фармацевтических наук

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Published
2019-12-27
How to Cite
1. Reshetov Y. Y., Ligacheva A. A., Avdeeva Y. Y., Danilets M. G., Golovchenko V. V., Trofimova Y. S., Gulina Y. I., Sherstoboev Y. Y., Gur’yev A. M., Rovkina K. I., Krivoshchekov S. V., Belousov M. V. POLYSACCHARIDES OF THREE SAUSSUREA DC SPECIES (S. CONTROVERSA, S. SALICIFOLIA, S. FROLOVII): ISOLATION, CHARACTERIZATION AND INFLUENCE ON NO-PRODUCING PROPERTIES OF MACROPHAGES // chemistry of plant raw material, 2019. № 4. P. 77-85. URL: http://journal.asu.ru/cw/article/view/5483.
Issue
No 4 (2019)
Section
Biopolymers of plants

Copyright (c) 2019 Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)

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