APPLICATION OF PICRIC ACID FOR THE QUANTITATIVE ANALYSIS OF CHAMAZULENE CONTENT IN MATRICARIA CHAMOMILLA FLOWERS

  • Татьяна (Tat'iana) Михайловна (Mikhailovna) Шишмарева (Shishmareva) Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Science, ul. Sakh’yanovoy, 6, Ulan-Ude, 670047 Email: shishmarevatm@rambler.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.201603568
Keywords: Matricaria chamomilla (L.) Rauschert, Asteraceae, picric acid, chamazulene, quantitative determination, spectrophotometry

Abstract

In this paper the standardization of raw of Matricaria chamomilla with application of picric acid is proposed. The technique of quantitative determination of chamazulene content in Matricaria chamomilla flowers with spectrophotometric method is developed. In the study of the absorption spectrum of an alcoholic solution of essential oil of Matricaria chamomilla after treatment by picric acid revealed that its maximum occurs at 657 nm and coincides with that of picrate chamazulene. The point at 657 nm is chosen as analytical wavelengths.

Experiments are conducted of "added-found" method to assess the accuracy of the developed technique and found that the relative error no more than 3,5%. The relative error of determination in series of successive measurements is no more than 2,5%, and in series of parallel measurements – 5,5%. Metrological analysis showed that the method is accurate and reproducible. The quantitative determination of chamazulene content defined in 6 batches of industrial raw materials using the developed technique. The total of chamazulene content in Matricaria chamomilla flowers is between 8,49–20,05 mg%. The chamazulene content in Matricaria chamomilla flowers in terms of absolutely dry raw material should be at least 8 mg%.

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Author Biography

Татьяна (Tat'iana) Михайловна (Mikhailovna) Шишмарева (Shishmareva), Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Science, ul. Sakh’yanovoy, 6, Ulan-Ude, 670047
Researcher, Laboratory of Biomedical Research, Candidate of Pharmaceutical Sciences

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Published
2016-06-29
How to Cite
1. Шишмарева (Shishmareva)Т. (Tat’iana) М. (Mikhailovna) APPLICATION OF PICRIC ACID FOR THE QUANTITATIVE ANALYSIS OF CHAMAZULENE CONTENT IN MATRICARIA CHAMOMILLA FLOWERS // chemistry of plant raw material, 2016. № 3. P. 95-101. URL: http://journal.asu.ru/cw/article/view/568.
Issue
No 3 (2016)
Section
Low-molecular weight compounds

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