QUANTITATIVE DETERMINATION OF THE AMOUNT OF CAROTENOIDS IN THE FRUITS OF CHINESE LYCIUM CHINENSE MILL.

UDC 615.322:543.422.3

  • Yevgeniy Yevgen'yevich Kurdyukov Penza State University Email: e.e.kurdyukov@mail.ru
  • Yelena Fedorovna Semenova Penza State University Email: sef1957@mail.ru
  • Inessa Yakovlevna Moiseyeva Penza State University Email: moiseeva_pharm@mail.ru
  • Natal'ya Aleksandrovna Gavrilova Penza State University Email: natashagavril121314@icloud.com
  • Tat'yana Andreyevna Ponomareva Penza State University Email: tanecha2016@gmail.com
DOI_disc_logo https://doi.org/10.14258/jcprm.2020036609
Keywords: Lycium Chinese, carotenoids, spectrophotometry, quantitative determination, β-carotene

Abstract

The objects of the study were Mature dried fruits of Chinese birch (Lycium chinense Mill., this. Solanaceae (Solanaceae)). Four samples were examined: No. 1 (Gifts of Pamir, Russia), No. 2 (Gullin Tianhe Pharmaceutical, China), No. 3 (A. J. Alliance, Russia), No. 4 (Globaltorg, Russia). The purpose of this work is to determine the amount of carotenoids in the fruits of Chinese by spectrophotometry.

The quantitative determination of the amount of carotenoids in the raw material of Chinese Dereza was carried out. Thin-layer chromatography was used to confirm the presence of carotenoids in the fruits of Dereza. The expediency of using the spectrophotometric method for the detection and quantitative determination of carotenoids in extracts from the fruits of Chinese birch is substantiated. The electron spectra of hexane, acetone and acetone-hexane solutions in the wavelength range 400–500 nm have a maximum optical density at 450±2 nm, characteristic of β-carotene. The optimal conditions of extraction of carotenoids from the raw materials of this plant (extractant – hexane; ratio "raw material–extractant" – 1 : 5; extraction time – 90 minutes; the degree of grinding of raw materials – 0.5 mm). It was determined that the error of a single determination of the content of carotenoids in the fruits of Chinese Dereza with a confidence probability of 95% is ±3,49%. It was revealed that the content of carotenoids in the raw material of Chinese Dereza varies in the range of 33–39 mg%.

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Author Biographies

Yevgeniy Yevgen'yevich Kurdyukov, Penza State University

кандидат фармацевтических наук, доцент кафедры общей и клинической фармакологии

Yelena Fedorovna Semenova, Penza State University

кандидат биологических наук, профессор кафедры общей и клинической фармакологии

Inessa Yakovlevna Moiseyeva, Penza State University

доктор медицинских наук, декан лечебного факультета, заведующая кафедрой общей и клинической фармакологии

Natal'ya Aleksandrovna Gavrilova, Penza State University

студентка

Tat'yana Andreyevna Ponomareva, Penza State University

студентка

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Published
2020-10-22
How to Cite
1. Kurdyukov Y. Y., Semenova Y. F., Moiseyeva I. Y., Gavrilova N. A., Ponomareva T. A. QUANTITATIVE DETERMINATION OF THE AMOUNT OF CAROTENOIDS IN THE FRUITS OF CHINESE LYCIUM CHINENSE MILL. // chemistry of plant raw material, 2020. № 3. P. 139-144. URL: http://journal.asu.ru/cw/article/view/6609.
Issue
No 3 (2020)
Section
Low-molecular weight compounds

Copyright (c) 2020 chemistry of plant raw material

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