ANTIMICROBIAL AND ANTIRADICAL ACTIVITY OF INDIVIDUAL FRACTIONS OF PINUS SIBIRICA DU TOUR AND ABIES SIBIRICA LEDEB., NATIVE TO THE SIBERIAN REGION
UDC 615.322:615.076.7:615.017
Abstract
By the method of exhaustive hydroparodistillation, essential oil was obtained from Siberian pine (Pinus sibirica Du Tour) and Siberian fir (Abies sibirica Ledeb.), growing on the territory of the Krasnoyarsk territory. Separate fractions of oil were obtained: the first one after 45 min from the beginning of distillation, the second – after 2 hours, the third – after 5 hours, the fourth-after 10 hours, the fifth fraction was collected after the end of hydrodistillation. The antimicrobial activity of separate fractions of essential oil of P. sibirica and A. sibirica was studied against strains of opportunistic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus 209p, Micrococcus luteus, Acinetobacter baumanii, Candida albicans. The performed studies showed that all the studied samples of essential oils showed either bactericidal or bacteriostatic activity against the strains of microorganisms taken in the experiment, except Pseudomonas aeruginosa. The type of activity displayed depended on the type of strain and sample of essential oil. It is noted that the sensitivity of the experimental strains to the components of the essential oils of P. sibirica and A. sibirica decreases during the transition from the first to the last fraction. We assume that, apparently, this is due to a decrease in the number of monoterpenes in the composition of oils.To study the antiradical activity, the reaction of essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. Antiradical activity of both whole essential oils of P. sibirica and A. sibirica and their separate fractions was established. There was an increase in antiradical activity with a decrease in the content of monoterpenes in the composition of essential oil.
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Isayev A.S. Materialy Vsesoyuznoy konferentsii «Razvitiye proizvoditel'nykh sil Sibiri i zadachi uskoreniya nauchno-tekhnicheskogo progressa». [Proceedings of the All-Union conference "Development of the productive forces of Sibe-ria and the task of accelerating scientific and technological progress"]. Krasnoyarsk, 1985, pp. 6–22. (in Russ.).
Yefremov Ye.A., Yefremov A.A. Khimiya rastitel'nogo syr'ya, 2010, no. 2, pp. 135–138. (in Russ.).
Yefremov Ye.A., Nazirov R.A., Yefremov A.A. Vestnik KrasGAU, 2014, no. 12, pp. 89–93. (in Russ.).
Yefremov A.A., Zykova I.D. Komponentnyy sostav efirnykh masel khvoynykh rasteniy Sibiri. [Component composition of essential oils of Siberian coniferous plants]. Krasnoyarsk, 2013, 133 p. (in Russ.).
Yefremov A.A., Strukova Ye.G., Narchuganov A.N. Journal of Siberian Federal University. Chemistry, 2009, no. 4, pp. 335–350. (in Russ.).
Samsonova N.A., Gusakova M.A., Bogolitsin K.G., Selivanova N.V. Sibirskiy lesnoy zhurnal, 2020, no. 2, pp. 31–39. (in Russ.).
Bogolitsin K.G., Gusakova M.A., Krasikova A.A., Samsonova N.A. Sbornik materialov dokladov Mezhdunarodnoy nauchno-tekhnicheskoy konferentsii «Khimiya i khimicheskaya tekhnologiya pererabotki rastitel'nogo syr'ya». [Collec-tion of reports of the International Scientific and Technical Conference "Chemistry and Chemical Technology of Pro-cessing Plant Raw Materials"]. Minsk, 2018, pp. 70–74. (in Russ.).
Ochocka J.R., Asztemborska M., Sybilska D., Langa W. Pharmaceutical Biology, 2002, vol. 40, no. 5, pp. 395–399.
Savel'yeva Ye.Ye., Yefremov A.A. Vestnik KrasGAU, 2017, no. 2, pp. 141–147. (in Russ.).
Misharina T.A., Alinkina Ye.S., Fatkullina A.K., Vorob'yeva L.D., Medvedeva I.B., Burlakova Ye.B. Prikladnaya bio-khimiya i mikrobiologiya, 2012, vol. 48, no. 1, pp. 117–123. (in Russ.).
Misharina T.A., Alinkina Ye.S., Medvedeva I.B. Prikladnaya biokhimiya i mikrobiologiya, 2015, vol. 51, no. 1, pp. 99–104. (in Russ.).
Alinkina Ye.S., Misharina T.A., Fatkullina A.K. Prikladnaya khimiya i mikrobiologiya, 2013, vol. 49, no. 1, pp. 82–87. DOI: 10.7868/S0555109913010029 (in Russ.).
Ruberto G., Baratta M.T. Food Chemistry, 2000, vol. 69, no. 2, pp. 167–174.
Yashin Ya.I., Ryzhnev V.Yu., Yashin F.Ya., Chernousova N.I. Prirodnyye antioksidanty. Soderzhaniye v pishchevykh produktakh i ikh vliyaniye na zdorov'ye, i stareniye cheloveka. [Natural antioxidants. Content in foods and their effects on human health and aging]. Moscow, 2009, 192 p. (in Russ.).
Vodolazova S.V., Myadelets M.A., Karpova M.R. Sibirskiy meditsinskiy zhurnal, 2011, vol. 26, no. 2, pp. 54–58. (in Russ.).
Gulyayev D.K., Novikova V.V., Belonogova V.D. Meditsinskiy al'manakh, 2015, no. 4 (39), pp. 231–214. (in Russ.).
Van Duijn P.J., Dautzenberg M.J., Oostdijk E.A. Curr. Opin. Crit Care., 2011, vol. 17, pp. 658–665.
Cushnie T.P., Lamb A.J. Int. J. Antimicrob. Agents., 2011, vol. 38, pp. 99–107.
Molyneux P. Songklanakarin J. Sci.Technol., 2004, vol. 26, no. 2, pp. 211–219.
MUK 4.12.1890-04. Opredeleniye chuvstvitel'nosti mikroorganizmov k antibakterial'nym preparatam: me-todicheskiye ukazaniya. [MUK 4.12.1890-04. Determination of the sensitivity of microorganisms to antibacterial drugs: methodolog-ical instructions]. Moscow, 2004, 91 p. (in Russ.).
Baratta M.T., Dorman H.J.D., Deans S.G., Figueredo A.C., Barosso J.G., Ruberto G. Flavour Fragrance Journal, 1998, vol. 13, no. 4, pp. 235–244.
Foti M.C., Ruberto G. Journal Agricultural Food Chem., 2001, vol. 49, no. 1, pp. 342–348.
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