DETERMINATION OF VINCAMINE AND AJMALICINE CONTENTS IN INTRODUCED IN BELARUS VINCA MI-NOR PLANTS BY HPLC-MS
Abstract
Vincamine and ajmalicine contents was determined by HPLC-MS in common periwinkle plants (Vinca minor L., Apocynaceae) cultivated in moderate continental climate conditions of Belarus. According to our experimental data, levels of terpenoid indole alkaloids (TIA) and vincamine in periwinkle leaves and herbal were comparable to TIA and vincamine concentrations in south plants. The pharmacologically valuable hypotensive alkaloid ajmalicine was identified in V. minor plants. Distributions of vincamine and ajmalicine were studied in different organs of periwinkle plants during the flowering period. It was foundthat aqueoussolutions of ethylalcohol (not less than 50%) and aceticacid(1-5%) are effective to extract indolealkaloid fromthe herbalmaterials. It was determined maximum yield of vincamineandajmaliсinein 96% ethanol and 5% acetic acid extracts.
Downloads
Metrics
References
Растительные ресурсы СССР: цветковые растения, их химический состав, использование. Л., 1990. Т. 5. 328 с.
Победимова Е.Г. и др. Флора СССР. Семейство Apocynaceae / под ред. Б.К. Шишкина, Е.Г. Боброва. М., 1952. С. 646–652.
Регистрация лекарственных средств России / под общ. ред. Г.А. Вышковского. М., 2004. 195 с.
Кухарева Л.В., Пашина Г.В. Полезные травянистые растения природной флоры: Справочник по итогам интро-дукции в Белоруссии. Мн., 1986. 215 с.
Джус М.А., Молчан О.В., Кухарева Л.В., Спиридович Е.В., Юрин В.М. Род Vinca L. (Apocynaceae) во флоре Беларуси // Украинский ботанический журнал. 2009. Т. 66, №6. С. 783–793.
Verma P., Khan S. A., Mathur A.K., Shanker K., Kalra A. Fungal endophytes enhanced the growth and production ki-netics of Vinca minor hairy roots and cell suspensions grown in bioreactor // Plant Cell Tiss. Organ Cult. 2014. Vol. 118. Pp. 257–268.
Hirata K., Mukai M., Goda S., Ishio-Kinugasa M., Yoshida K., Sakai A., Miyamoto K. Cryopreservation of hairy root cultures of Vinca minor (L.) by encapsulation-dehydration // Biotechnology Letters. 2002. Vol. 24. Pp. 371–376.
Boyadzhiev L. et al. Extraction of vincamine from Periwinkle (Vinca minor L.): obtaining of total extract // Comptes rendus de L’Academie bulgare des Sciences. 2002. Vol. 55 (12). Pp. 49–52.
Лешина Л.Г., Булко О.В. Agrobacterium rhisogenes-опосредованная трансформация и регенерация двух видов растений из семейства Apocynaceae // Физиология и биохимия культурных растений. 2011. Т. 43, №6. С. 533–539.
Proksa B. et al. High performance liquid chromatographic determination of alkaloids from Vinca minor // Phytochemical Analisis. 1991. Vol. 2. Pp. 74–76.
Loyola-Vargas V.M. et al. Catharanthus biosynthetic enzymes: the road ahead // Phytochemistry Reviews. 2007. Vol. 6. Pp. 307–339.
Погоцкая А.А., Бузук Г.Н. Сравнительная характеристика влияния различных концентраций хлористоводород-ной и уксусной кислот на извлечение алкалоидов из листьев маклейи сердцевидной // Вестник фармации. 2010. Т. 48, №2. С. 39–48.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
