STUDY OF THE PROCESS EXTRACTION OF HYPERICIN FROM THE AERIAL PARTS OF HYPERICUM SCABRUM AND HYPERICUM PERFORATUM
UDC 547.672. 633.511:631.8
Abstract
The process extraction of hypericin from the aerial parts of Hypericum scabrum and Hypericum perforatum was studied. On the basis results research, the following extraction conditions were established: extractant – 80% ethanol, particle size of raw material – no more than 8 mm and process temperature – 20–40 °C. By the method of mathematical planning of the experiment according to Box-Wilson, the extraction conditions were determined, which ensure the extraction of hypericin from the raw material with a yield of 95%. Proposed a five-fold extraction of hypericin from the aerial parts of H. scabrum and H. perforatum with the necessary infusion at the first contact of the phases – 6 hours, at the second – 5 hours, at the third and fourth – 4 hours and at the fifth – 2 hours. The proposed a united technology for obtaining a «Dry extract of Hypericum» from the aerial parts of H. perforatum and H. scabrum. The proposed conditions for the extraction of hypericin were tested at the Technology center for GMP requirements by the Institute of the Chemistry of Plant Substances, of the Academy of Sciences Republic of Uzbekistan. More than 500.0 kg of the substance «Dry extract of Hypericum» adequate of Technical Conditions 03535440-022: 2016 has been produced.
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Rastitel'nyye resursy SSSR: Tsvetkovyye rasteniya, ikh khimicheskiy sostav, ispol'zovaniye; Sem-va Paeoniaceae – Thymelaeceae. [Plant resources of the USSR: Flowering plants, their chemical composition, use; Family Paeoniaceae – Thymelaeceae]. Leningrad, 1991, vol. 2, p. 11. (in Russ.).
Flora Uzbekistana. Institut botaniki Akademii nauk Uzbekskoy SSR. [Flora of Uzbekistan. Institute of Botany of the Academy of Sciences of the Uzbek SSR]. Tashkent, 1955, vol. 4, 717 p. (in Russ.).
Faizullina R.R. Fitokhimicheskoye izucheniye zveroboya prodyryavlennogo (Hypericum perforatum L.) flory Bashkortostana i perspektivy sozdaniya na yego osnove novykh lekarstvennykh sredstv: Dis. … kand. farm. nauk. [Phy-tochemical study of St. John's wort (Hypericum perforatum L.) of the flora of Bashkortostan and the prospects for creat-ing new drugs on its basis: Dis. ... Cand. farm. sciences]. Ufa, 2005, 169 p. (in Russ.).
Ibragimov T.I., Begimova D.I., Abdurakhmanov B.A., Ismailova M.G. Materialy konf. molodykh uchenykh «Aktual'nyye problemy khimii prirodnykh soyedineniy», posvyashchennykh pamyati akademika S.Yu. Yunusova. [Mate-rials of the conf. young scientists "Actual problems of the chemistry of natural compounds" dedicated to the memory of academician S.Yu. Yunusov]. Tashkent, 2015, p. 159. (in Russ.).
Sharopov F.S., Gulmurodov I.S., Setzer W.N. Journal of chemical and pharmaceutical research, 2010, vol. 2(6), pp. 284–290.
Savikin K., Dobric S., Tadic V., Zdunic G. Phytotherapy research, 2010, vol. 21, pp. 176–180. DOI:10.1002/ptr.2041.
Saddiqe Z., Naeem I., Maimoona A. Journal Ethnopharmacology, 2010, vol. 131, pp. 511–521. DOI: 10.1016/j.jep.2010.07.034.
Axarlis S., Mentis A., Demetzos C., Mitaku S., Skaltsounis A.L., Marselos M., Malamas M. Phytotherapy Research, 1998, vol. 12, pp. 507–511. DOI: 10.1002/(SICI)1099-1573(199811)12:7507::AID-PTR3703.0.CO;2-H.
Vasil'chenko V.A., Vasil'yeva L.N., Komissarenko N.F. i dr. Rastitel'nyye resursy, 1986, vol. 22, no. 1, pp. 12–21. (in Russ.).
Maksyutina N.P. Rastitel'nyye lekarstvennyye sredstva. [Herbal remedies]. Kiev, 1985, pp. 176–177. (in Russ.).
Demisch L., Holzl J., Gollink B., Kaczmarryk P. Pharmacopsychiatry, 1989, vol. 22, no. 5, p. 194.
Kkhaled A.Z. Provizor, 2003, no. 10, pp. 23–27. (in Russ.).
Kortikov V.N., Kortikov A.V. Lekarstvennyye rasteniya. [Medicinal plants]. Moscow, 1999, pp. 213–216. (in Russ.).
Kucherov Ye.V., Lazareva D.N., Desyatkin V.K. Lekarstvennyye rasteniya Bashkirii: ikh ispol'zovaniye i okhrana. [Medicinal plants of Bashkiria: their use and protection]. Ufa, 1989, pp. 86–90. (in Russ.).
Lovkova M.L., Rabinovich A.M., Buzuk T.N. i dr. Pochemu rasteniya lechat. [Why plants are treated]. Moscow, 1989, 252 p. (in Russ.).
Khalmatov Kh.Kh., Kharlamov I.A., Alimov Kh.I. i dr. Tez. dokl. 2 s"yezda farmatsevtov Uzbekistana. [Tez. report 2 congresses of pharmacists of Uzbekistan]. Tashkent, 1982, pp. 105–106. (in Russ.).
Kirilyuk Zh.I. Vestnik khirurgii, 1978, no. 4, pp. 126–130. (in Russ.).
Agostinis P., Vantieghem A., Merlevede W., de Witte P.A.M. The International Journal of Biochemistry & Cell Biolo-gy, 2002, vol. 34, pp. 221–241. DOI: 10.1016/S1357-2725(01)00126-1.
Bach-Rojecky L., Kalodera Z., Samarzija I. Acta Pharmaceutica, 2004, vol. 54, no. 2, pp. 157–162.
Kurkin V.A., Pravdivtseva O.Ye. Zveroboy: itogi i perspektivy sozdaniya lekarstvennykh sredstv. [St. John's wort: re-sults and prospects for the creation of medicines]. Samara, 2008, 127 p. (in Russ.).
Patent 2327481 (RU). 2008. (in Russ.).
Pravdivtseva O.Ye., Kurkin V.A. Khimiya rastitel'nogo syr'ya, 2011, no. 4, pp. 333–334. (in Russ.).
Patent 2623084 (RU). 2017. (in Russ.).
Babin A.V., Rakipov D.F. Organizatsiya i matematicheskoye planirovaniye eksperimenta. [Organization and mathe-matical planning of the experiment]. Yekaterinburg, 2014, pp. 85–94. (in Russ.).
Ruzinov L.P. Statisticheskiye metody optimizatsii khimicheskikh protsessov. [Statistical methods for the optimization of chemical processes]. Moscow, 1972, 182 p. (in Russ.).
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