STUDY OF THE AMINO ACID AND ELEMENTAL COMPOSITION OF THE COMPLEX DRY EXTRACT "PHY-TOINFLAM"
UDC 615.322:577.112.3:543.632.4
Abstract
The purpose of this research is to study the amino acid and elemental composition of the complex dry extract "Phytoinflam" obtained from ordinary Oak (latin. Quercus robur L.). Chamomile (latin. Chamоmilla recutita (L.)) and three-lobe Beggarticks (latin. Bidens tripartita).
Determination of the amino acid composition was carried out by high performance liquid chromatography (HPLC), and the quantitative determination of macro-and microelements - by the method of inductively coupled plasma mass spectrometry (ICP-MS)
According to the results of the research, it was revealed that 20 amino acids were found in the dry extract "Phytoinflam": 15 of which are representatives of various classes of aliphatic amino acids, 3 – aromatic and 2 – heterocyclic. It should be noted that ten out of twenty discovered amino acids are irreplaceable. The largest quantities are proline, valine, alanine, arginine, cysteine and glutamine. The total amino acid content was 14.767 mg/100 mg, of which 38.97% were essential amino acids, and, accordingly, 61.03% were non-essential.
The results of the study of micro- and macroelements showed the presence of 28 elements in the analyzed extract, among which seven are essential (Co, Cr, Cu, Fe, Mn, Se, Zn) and four conditionally essential. It is noted that the high content of such elements important for the vital activity of the body as calcium, potassium, sodium, magnesium, silver, strontium. The concentrations of heavy metals and arsenic in the analyzed dry extract did not exceed the regulated limit according to WHO and state Pharmacopoeia XIV.
The data obtained indicate the high value of the complex dry extract "Phytoinflam", which confirms its therapeutic value and the possibility of creating drugs based on it.
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