STRUCTURAL-CHEMICAL CHARACTERISTIC AND BIOLOGICAL ACTIVITY OF POLYSACCHARIDES FROM HERACLEUM SOSNOWSKYI MANDEN
Abstract
Was studied character of the changes yield and the monosaccharide composition the arabinogalactan proteins (AGP), pectic polysaccharides and of cross-linking glycans, were isolated from the aboveground part of Heracleum sosnowskyi Manden the sequential treatment with water, HCl solution, and (NH4)2C2O4, KOH and NaOH solution. The fractions were isolated extraction with water consist mainly of AGP and minor amounts of pectic polysaccharides. The fractions were isolated extraction with aqueous HCl solution are a mixture of AGP and pectic polysaccharides, the latter prevailing. The fractions were isolated extraction with aqueous (NH4)2C2O4 are characterized by the predominance of pectic polysaccharides and a minor amount of AGP.
When studying the monosaccharide composition of the fractions extracted KOH solution determined that the main components of their carbohydrate chains are residues of xylose, glucose and uronic acids that probably specifies the affiliations these fractions of cross-linking glycans class glucuronoarabinoxylans and xyloglucans. The major components of the carbon chain fractions extracted aqueous solution of NaOH, are of mannose and glucose residues, which probably specifies the affiliations these fractions of cross-linking glycans class glucomannan. The last two fractions also contained some amount of pectin polysaccharides.
It is shown high biological activity pectic polysaccharides, from the aboveground part of Heracleum sosnowskyi Manden, extraction (NH4)2C2O4 solution on germination and growth rate of seeds, germs and roots of Triticum aestivum L.
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Медведева Е.Н., Бабкин В.А., Остроухова Л.А. Арабиногалактан лиственницы – свойства и перспективы ис-пользования // Химия растительного сырья. 2003. №1. С. 27–27.
Kumar, S., Gupta, P., Sharma, S., Kumar, D. A review on immunostimulatory plants // J. of Chin. Integrative Medicine. 2011. Vol. 9. Pp. 117–128.
Senejoux, F., Demougeot, C., Cuciureanu, M., Miron, A., Cuciureanu, R., Berthelot, A., et al. Vasorelaxant effects and mechanisms of action of Heracleum sphondylium L. (Apiaceae) in rat thoracic aorta // J. Ethnopharmacol. 2013. Vol. 147. Pp. 536–539.
Патент 2057541 (RU). Средство для лечения экзематозов / А.И. Суханов. 1996.
Патент 2076726 (RU). Способ лечения псориаза / А.И. Суханов. 1997.
Михайлова Е.А., Щербакова Т.П., Шубаков А.А. Изучение эффективности применения препаратов пектиновых полисахаридов на разнотравье в условиях полевого опыта // Научные достижения биологии, химии, физи-ки: материалы Междун. заочной науч.-практ. конф. Новосибирск, 2012. 114 с.
Shakhmatov E.G., Toukach P.V., Kuznetsov S.P., Makarova E.N. Structural characteristics of water-soluble polysac-charides from Heracleum sosnowskyi Manden // Carbohydr. Polym. 2014. Vol. 102, Pp. 521–528.
Shakhmatov E.G., Atukmaev K.V., Makarova E.N. Structural characteristics of pectic polysaccharides and arabinoga-lactan proteins from Heracleum sosnowskyi Manden // Carbohydr. Polym. 2016. Vol. 136, Pp. 1358–1369.
Dubois M., Qilles K.A., Hamilton J.K., Rebers P.A., Smith F. Colorimetric method for determination of sugars and re-lated substances // Analyt. Chem. Vol. 28. Pp. 350–356.
Usov A.I., Bilan M.I., Klochkova N.G. Polysaccharide ciomposition of several calcareous red algae: Isolation of algi-nate from corallina pilutitara // Bot. Marina. 1995. Vol. 38. Pp. 43–51.
Bradford H.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the prin-cipal of protein-dye-binding // Anal. Biochem. 1976. Vol. 72, Pp. 248–254.
York W.S., Darvill A.G., McNeil M.A., Stevenson T.T., Albercheim P. Isolation and characterization of plant cell walls and cell-wall components // Methods Enzymol. 1985. Vol. 118. Pp. 3–40.
Дудкин М.С., Громов В.С., Ведерников Н.А., Каткевич Р.Г., Черно Н.К. Гемицеллюлозы. Рига, 1991. 488 с.
Ebringerova A., Hromadkova Z., Heinze T. Hemicellulose // Adv. Polym. Sci. 2005. Vol. 186, Pp 1–67.
Doering, A., Lathe, R., Persson, S. An update on xylan synthesis // Mole. Plant. 2012. Vol. 5. Pp. 769–771.
Prabasari I., Pettolino F., Liao M.-L., Bacic A. Pectic polysaccharides from mature orange (Citrus sinensis) fruit albedo cell walls: Sequential extraction and chemical characterization // Carbohydr. Polym. 2011. Vol. 84. Pp. 484–494.
Zablackis E., Huang J., Muller B., Darvill A.G., Albersheim P. Characterization of the cell-wall polysaccharides of Ar-abidopsis thaliana leaves // Plant Physiol. 1995. Vol. 107. Pp. 1129–1138.
Шахматов Е.Г., Макарова Е.Н., Михайлова Е.А., Шубаков А.А, Оводов Ю.С. Общая химическая характери-стика полисахаридов плодов граната (Punica granatum L.) // Бутлеровские сообщения. 2013. Т. 33, №3. С. 116–121.
Shakhmatov E. G., Toukach P. V., Michailowa E. A., Makarova E. N. Structural studies of arabinan-rich pectic poly-saccharides from Abies sibirica L. Biological activity of pectins of A. Sibirica // Carbohydr. Polym. 2014. Vol. 113. Pp. 515–524.
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