PEAT HUMIC ACIDS – PROSPECTIVE BIOLOGICALLY ACTIVE SUBSTANCES WITH ANTIOXIDANT ACTIVITY FOR THE DEVELOPMENT OF PROTECTIVE AGENTS
UDC 631.41:631.417
Abstract
A present study was carried out to investigate the antioxidant activity (AOA) of humic acids (HA) isolated by sodium hydroxide and sodium pyrophosphate from nine peat species of the Tomsk region different by botanical composition, degree of decomposition and ash content. All HA samples had shown the AOA in the study. As the results of four research methods, it was found that HAs are highly effective in inhibiting the free ABTS•+ radical cation, superoxide anion radical O2-•, hydroxyl radical HO•, and are able to bind Fe2+ in a wide range of concentrations. The activity of HAs in different tests was not equal between samples; HAs isolated by sodium hydroxide have had a higher AOA in inhibition of the superoxide anion–radical O2-• in the model reaction compared to HAs isolated by sodium pyrophosphate within the same peat species. In the study of iron binding activity, an inverse relationship was observed. In the model reactions of the free ABTS•+ radical cation and the hydroxyl radical HO• inhibition, a comparable level of activity was registered between the HA samples isolated by different solutions. Such an uneven distribution of the activity between various HA samples can be explained by the unequal chemical parameters of their structure, which depend on their origin and method of isolation. Using of such classical antioxidants with an established mechanism of action as comparison drugs, as a water-soluble analogue of tocopherol - "Trolox", a water-soluble antioxidant – ascorbic acid, a classic chelator – ethylenediaminetetraacetic acid (EDTA), a classic trap of a hydroxyl radical – mannitol, it is possible to conclude that the investigated HAs are effective antioxidants belonging to the groups of proton donors and complexing agents.
Downloads
Metrics
References
Zykova M.V., Logvinova L.A., Belousov M.V. Traditsionnaya meditsina, 2018, vol. 53, no. 2, pp. 27–38. (in Russ.).
Buzlama A.V., Chernov Yu.N. Eksperimental'naya i klinicheskaya farmakologiya, 2010, vol. 73, no. 9, pp. 43–48. DOI: 10.30906/0869-2092-2010-73-9-43-48. (in Russ.).
Visser S.A. International Humic Substances Society meeting. Sevilla, 1988, pp. 89–135.
Zykova M.V., Schepetkin I.A., Belousov M.V., Krivoshchekov S.V., Logvinova L.A., Bratishko K.A., Yusubov M.S., Romanenko S.V., Quinn M.T. Molecules, 2018, vol. 23, no. 4, pp. 753–768. DOI: 10.3390/molecules23040753.
Avvakumova N.P., Gerchikov A.Y., Khairullina V.R., Zhdanova A.V. Pharm. Chem. J., 2011, vol. 45, pp. 192–193. DOI: 10.1007/s11094-011-0590-2.
Vaškova J., Veliká B., Pilátová M., Kron I., Vaško L. In Vitro Cell. Dev. Biol., 2011, vol. 47, no. 5-6, pp. 378–382. DOI: 10.1007/s11626-011-9405-8.
Martinovich G.G., Cherenkevich S.N. Okislitel'no-vosstanovitel'nyye protsessy v kletkakh. [Redox processes in cells]. Minsk, 2008, 159 p. (in Russ.).
Buzlama V.S., Dolgopolov V.N., Safonov A.V., Buzlama S.V. Sbornik dokladov konferentsii «Itogi i perspektivy primeneniya guminovykh preparatov v produk-tivnom zhivotnovodstve, konevodstve i ptitsevodstve». [Collection of re-ports of the conference "Results and prospects of the use of humic preparations in productive animal husbandry, horse breeding and poultry farming"]. Moscow, 2006, pp. 24–35. (in Russ.).
Piotrowska D., Dlugosz A., Witkiewicz K., Pajak J. Acta Pol. Pharm., 2000, vol. 57, pp. 127–129.
Gau R.-J., Yang H.-L., Suen J.-L., Lu F.-J. Biochemical and Biophysical Research Communications, 2001, vol. 283, no. 4, pp. 743–749. DOI: 10.1006/bbrc.2001.4832.
Buzlama A.B., Chernov Yu.N., Dronova Yu.M., Astanina M.A. Nauchnyye vedomosti BelGU, 2011, vol. 22, no. 17, pp. 214–221. (in Russ.).
Lotosh T.D. Biologicheskiye nauki, 1991, no. 10, pp. 99–103. (in Russ.).
Belousov M.V., Akhmedzhanov R.R., Zykova M.V., Gur’ev A.M., Yusubov M.S. Pharm. Chem. J., 2014, vol. 48, no. 4, pp. 249–252. DOI: 10.1007/s11094-014-1088-5.
Vetvicka V., Vashishta A., Fuentes M., Baigorri R., Jose M.G.-M., Yvin J.-C. J. Med. Food., 2013, vol. 16, no. 7, pp. 625–632. DOI: 10.1089/jmf.2012.0212.
Maslinski C., Fogel W.A., Andjewski W. Acta Pol. Pharm., 1995, vol. 50, no. 4-5, pp. 413–416.
Avvakumova N.P., Zhdanova A.V., Glubokova M.N., Zhernov Yu.V. Izvestiya Samarskogo nauchnogo tsentra Rossiyskoy akademii nauk, 2011, vol. 13, no. 1, pp. 1960–1963. (in Russ.).
Ozcan A., Sen H.M., Sehitoglu I., Alacam H., Guven M., Aras A.B., Akman T., Silan C., Cosar M., Karaman H.I.O. Inflammation, 2015, vol. 38, no. 1, pp. 32–39. DOI: 10.1007/s10753-014-0005-0.
Akbas A., Silan C., Gulpinar M.T., Sancak E.B., Ozkanli S.S., Cakir D.U. Inflammation, 2015, vol. 38, pp. 2042–2048. DOI: 10.1007/s10753-015-0185-2.
Buzlama A.B. Sbornik statey Vserossiyskoy nauch.-praktich. konf. s mezhdunarodn. uchastiyem «Ot fundamental'nykh issledovaniy k inno-vatsionnym meditsinskim tekhnologiyam». [Collection of articles of the All-Russian scientific-practical. conf. from international participation "From fundamental research to innovative medical technologies"]. St.-Petersburg, 2010, pp. 15–16. (in Russ.).
Wollina U. Br. J. Dermatol., 1999, vol. 140, pp. 561–562. DOI: 10.1046/j.1365-2133.1999.02744.x.
Zykova M.V., Belousov M.V., Lasukova T.V., Gorbunov A.S., Logvinova L.A., Dygay A.M. Byulleten' eksperi-mental'noy biologii i meditsiny, 2017, vol. 163, no. 2, pp. 167–170. (in Russ.).
Belousov M.V., Akhmedzhanov R.R., Zykova M.V., Arbuzov A.N., Gur’ev A.M., Yusubov M.S. Pharm. Chem. J., 2014, vol. 48, pp. 97–99. DOI: 10.1007/s11094-014-1056-0.
Buzlama A.B., Chernov Yu.N., Slivkin A.I. Vestnik Voronezhskogo gosudarstvennogo universiteta, 2010, no. 1, pp. 140–145. (in Russ.).
Belousov M.V., Akhmedzhanov R.R., Zykova M.V., Vasil’ev K.Yu., Yusubov M.S. Pharm. Chem. J., 2015, vol. 49, no. 4, pp. 250–253. DOI: 10.1007/s11094-015-1265-1.
Zykova M.V., Logvinova L.A., Krivoshchekov S.V., Voronova O.A., Lasukova T.V., Bratishko K.A., Zholobova G.A., Golubina O.A., Perederina I.A., Drygunova L.A., Tveryakova Ye.N., Belousov M.V. Khimiya rastitel'nogo syr'ya, 2018, no. 3, pp. 239–250. DOI: 10.14258/jcprm.2018033925. (in Russ.).
Gupta D. Intern. J. of Pharmaceutical Sciences and Research, 2015, vol. 6, no. 2, pp. 546–566. DOI: 10.13040/IJPSR.0975-8232.6(2).546-66.
Chesnokova N.P., Ponukalina Ye.V., Bizenkova M.N. Uspekhi sovremennogo yestestvoznaniya, 2006, no. 7, pp. 29–36. (in Russ.).
Kajarabille N., Latunde-Dada G.O. Int. J. Mol. Sci., 2019, vol. 20(19), p. 4968. DOI: 10.3390/ijms20194968.
Matros A., Peshev D., Peukert M., Mock H.P., Van den Ende W. Plant J., 2015, vol. 82(5), pp. 822–839. DOI: 10.1111/tpj.12853. PMID: 25891826.
Bilenko M.V. Ishemicheskiye i reperfuzionnyye povrezhdeniya organov. [Ischemic and reperfusion injuries of organs]. Moscow, 1989, 368 p. (in Russ.).
Patent 2610446 (RU). 13.02.2017. (in Russ.).
Bentayeb K., Rubio C., Nerín C. Analyst, 2012, vol. 137, no. 2, pp. 459–470. DOI: 10.1039/c1an15505a.
Schepetkin I., Potapov A., Khlebnikov A., Korotkova E., Lukina A., Malovichko G., Kirpotina L., Quinn M.T. Journal of Biological Inorganic Chemistry Scientific Journal, 2006, vol. 11, pp. 499–513. DOI: 10.1007/s00775-006-0101-1.
Mahakunakorn P., Tohda M., Murakami Y., Matsumoto K., Watanabe H. Biological and Pharmaceutical Bulletin, 2004, vol. 27, no. 1, pp. 38–46. DOI: 10.1248/bpb.27.38.
Treml J., Šmejkal K. Comprehensive reviews in food science and food safety, 2016, vol. 15, no. 4, pp. 720–738. DOI: 10.1111/1541-4337.12204.
![](http://journal.asu.ru/public/journals/1/article_8784_cover_ru_RU.png)
Copyright (c) 2021 chemistry of plant raw material
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.