PEAT HUMIC ACIDS – PROSPECTIVE BIOLOGICALLY ACTIVE SUBSTANCES WITH ANTIOXIDANT ACTIVITY FOR THE DEVELOPMENT OF PROTECTIVE AGENTS

UDC 631.41:631.417

  • Kristina Aleksandrovna Bratishko Siberian State Medical University; National Research Tomsk Polytechnic University Email: kr-1295@mail.ru
  • Mariya Vladimirovna Zykova Siberian State Medical University http://orcid.org/0000-0002-1973-8983 Email: gmv2@rambler.ru
  • Vladimir Vladimirovich Ivanov Siberian State Medical University Email: ivanovvv1953@gmail.com
  • Evgeniy Evgen'yevich Buyko Siberian State Medical University; National Research Tomsk Polytechnic University Email: buykoevgen@yandex.ru
  • Larisa Aleksandrovna Drygunova Siberian State Medical University Email: l_drygunova@mail.ru
  • Irina Vasil'yevna Perminova Moscow State University M.V. Lomonosov Email: iperminova@gmail.com
  • Mikhail Valer'yevich Belousov Siberian State Medical University; National Research Tomsk Polytechnic University Email: mvb63@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.2021018784
Keywords: antioxidants, free radicals, humic acids, peat, colorimetry, proton donors, complexing agents

Abstract

A present study was carried out to investigate the antioxidant activity (AOA) of humic acids (HA) isolated by sodium hydroxide and sodium pyrophosphate from nine peat species of the Tomsk region different by botanical composition, degree of decomposition and ash content. All HA samples had shown the AOA in the study. As the results of four research methods, it was found that HAs are highly effective in inhibiting the free ABTS•+ radical cation, superoxide anion radical O2-•, hydroxyl radical HO•, and are able to bind Fe2+ in a wide range of concentrations. The activity of HAs in different tests was not equal between samples; HAs isolated by sodium hydroxide have had a higher AOA in inhibition of the superoxide anion–radical O2-• in the model reaction compared to HAs isolated by sodium pyrophosphate within the same peat species. In the study of iron binding activity, an inverse relationship was observed. In the model reactions of the free ABTS•+ radical cation and the hydroxyl radical HO• inhibition, a comparable level of activity was registered between the HA samples isolated by different solutions. Such an uneven distribution of the activity between various HA samples can be explained by the unequal chemical parameters of their structure, which depend on their origin and method of isolation. Using of such classical antioxidants with an established mechanism of action as comparison drugs, as a water-soluble analogue of tocopherol - "Trolox", a water-soluble antioxidant – ascorbic acid, a classic chelator – ethylenediaminetetraacetic acid (EDTA), a classic trap of a hydroxyl radical – mannitol, it is possible to conclude that the investigated HAs are effective antioxidants belonging to the groups of proton donors and complexing agents.

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Author Biographies

Kristina Aleksandrovna Bratishko, Siberian State Medical University; National Research Tomsk Polytechnic University

аспирант, ассистент кафедры химии

Mariya Vladimirovna Zykova, Siberian State Medical University

доктор фармацевтических наук, доцент, заведующий кафедрой химии, профессор кафедры фармацевтического анализа

Vladimir Vladimirovich Ivanov, Siberian State Medical University

кандидат биологических наук, руководитель центра доклинических исследований

Evgeniy Evgen'yevich Buyko, Siberian State Medical University; National Research Tomsk Polytechnic University

аспирант, ассистент кафедры биохимии и молекулярной биологии с курсом клинической лабораторной диагностики, лаборант центра доклинических исследований

Larisa Aleksandrovna Drygunova, Siberian State Medical University

кандидат химических наук, доцент кафедры фармацевтического анализа, доцент кафедры химии

Irina Vasil'yevna Perminova, Moscow State University M.V. Lomonosov

главный научный сотрудник научно-исследовательской лаборатории природных гуминовых систем

Mikhail Valer'yevich Belousov, Siberian State Medical University; National Research Tomsk Polytechnic University

доктор фармацевтических наук, заведующий кафедрой фармацевтического анализа

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Published
2021-03-16
How to Cite
1. Bratishko K. A., Zykova M. V., Ivanov V. V., Buyko E. E., Drygunova L. A., Perminova I. V., Belousov M. V. PEAT HUMIC ACIDS – PROSPECTIVE BIOLOGICALLY ACTIVE SUBSTANCES WITH ANTIOXIDANT ACTIVITY FOR THE DEVELOPMENT OF PROTECTIVE AGENTS // chemistry of plant raw material, 2021. № 1. P. 287-298. URL: http://journal.asu.ru/cw/article/view/8784.
Issue
No 1 (2021)
Section
Peat and products of its processing

Copyright (c) 2021 chemistry of plant raw material

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