ALLERGENIC PROFILE OF BIRCH POLLEN (BETULA PENDULA ROTH) FULL EXTRACT: STUDY OF IDENTIFI-CATION AND QUANTIFICATION METHODOLOGICAL PROCEDURES FOR BETV1 MAJOR PROTEIN BY LC-MS
UDC 615.322:543.544+615.218.3:582.632.1
Abstract
Since ancient times, silver birch, Betula pendula Roth (Betulaceae L.), is used in medicine practice, thanks to a wide range of bioactive compounds presented in almost all parts of the plant. However, birch pollen is the cause of severe allergic diseases - hay fever. The report provides data concerning the standardization of the full birch pollen allergen extract composition. This biological product is the sum of allergenic proteins (Betv), is used for allergen-specific immunotherapy (SIT). Since the Russian standardization protocols of the allergen extract did not meet the requirements of the modern world, it had become necessary to develop a sensitive method for the quantitative determination of the main allergenic protein Betv1 using modern physical and chemical methods, such as LC-MS.
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