LIGNANS OF OIL EXTRACT FROM SCHISANDRA CHINENSIS TURCZ. (BAILL.) SEEDS
Abstract
Fruits and seeds of Schisandra chinensis Turcz. (Baill.), fam. Schisandraceae are well known as adaptogens. Biological activity of Shisandra fruits and seeds is caused by special group of chemical compounds – lignans (shisandrins). The aim of the present work was to estimate possibility of extraction of lignans from S. chinensis seeds by fatty oils and further medicinal application of obtained extract.
Extract from S. chinensis seeds with various fatty oils – corn oil, olive oil and helianthus oil, were obtained by original technology with rotor-pulsation apparatus. Qualitative and quantitative analysis was done by RP-HPLC method with standards of five shisandrins. Shisandrol A (about 0.1%), shisandrol B (about 0.083%), shisantherin А (about 0,022%), shisandrin А (about 0,018%), shisandrin B (about 0,09%) were identified in extracts. Total content of identified shisandrins was about 0.3%. There were not any significant differences on shisandrins composition for extracts received with use of various fatty oils. Relations between individual lignans were about 1:1:0,2:0,2:1 and is optimal for adaptogenic properties of Shisandra drugs.
In biological experiments it was shown that oil extract of S. chinensis seeds is non toxic, safe for application, its pharmacological efficiency was revealed. Methods of standardization of extract as pharmaceutical substance were proposed and validated.
It was estimated that density, refraction index and fatty acids composition (according to TLC after EP 6.0 test)) for initial oil and oil extract are identical or differ slightly, and the acid value considerably increases due to extractive substances from S. chinensis seeds. Application of single extraction procedure by methanol is proved for extract sample treatment for shisandrins quantification.
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