Аннотация
This article presents the results of a study assessing the state of morphological changes in the male generative organs of Scots pine (Pinus sylvestris L.) in Kazakhstan and Poland. The purpose of this study is to assess the transformation of pine pollen under different environmental conditions. Research and pollen sampling were carried out in 2023 in different environmental conditions and in different geographical areas: Astana (Kazakhstan), Bayanaul State National Natural Park (BSNNP) (Kazakhstan) and Poznan (Poland). For cytological analysis, microsporophylls were selected from the middle part of the microstrobilus, the material was stained with 1% Lugol's pharmaceutical solution, the preparations were studied on a Nikon eclipse E 200 microscope with a magnification of 10x15x40. The results of the analysis showed that the maximum number of disturbances in pine pollen was detected in samples from Poznan - 63%. In the Bayanaul Natural Park and in Astana, pine has fewer anomalies in pollen - 57% and 55%. The most common types of anomalies found in Scots pine pollen in the study areas are: deformed (26.3%), pollen with impaired shell formation (19%) and degraded (7.4%). Under conditions of technogenic pollution in coniferous trees (Pinus sylvestris L.), a higher level of abnormality of pollen grains was revealed than in relatively clean conditions, and a wide spectrum (sterile, deformed and small pollen grains with disturbances (without air sacs, with one and three air bags).
Литература
Assylbekova GE (2012) Evaluation of types of pollen grain anomalies of common pollen (Pinus sylvestris l.) in the territory of the urban ecosystem of Pavlodar. Russnauka. Ecologia 6. [In Russian]
Benca JP, Duijnstee IAP, Looy CV (2018) UV-B-induced forest sterility: Implications of ozone shield failure in Earth’s largest extinction. Science Advances 4: 1–10. https://doi.org/10.1126/sciadv.1700618
Chropeňová M, Klemmová Gregušková E, Karásková P, Přibylová P, Kukučka P, Baráková D, Čupr P (2016) Pine needles and pollen grains of Pinus mugo Turra – A biomonitoring tool in high mountain habitats identifying environmental contamination. Ecological Indicators 66: 132–142. https://doi.org/10.1016/j.ecolind.2016.01.004
Dzjuba OF (2006) Pollination indication of environmental quality. Nedra, Saint Petersburg, 197 pp. [In Russian]
Environmental protection in the Republic of Kazakhstan 2018-2022 (2023) Statistical compilation. Bureau of National statistics of the Agency for Strategic planning and reforms of the Republic of Kazakhstan, Astana, 111–137.
Hlebova LP, Bychkova OV (2016) Peculiarities of development of male gametophyte of Scots pine Rinus sylvestris L. in urban environment. Ukrainian Journal of Ecology 6: 17–23. http://dx.doi.org/10.15421/201623 [In Russian]
Hlebova LP, Ereshhenko OV (2012) The quality of pollen of silver birch (Betula pendula Rotn.) in the conditions of Barnaul. Izvestiya of Altai State University 3/1: 75–81. [In Russian]
Hofman M, Wachowski L (2010) Soil Concentrations of Platinum and Lead Along the Main Exit Routes from the City of Poznan. Ochrona Srodowiska 32/3: 43–47.
Kalashnik NA (2011) Pollen anomalies in Siberian spruce under different ecological conditions. Scientific news. Natural Sciences Series 3 (14/1): 105–109. [In Russian]
Kalashnik NA, Jasovieva SM, Presnuhina LP (2008) Coniferous tree pollen anomalies in industrial pollution in the Southern Urals. Forestry [Lesovedenie] 2: 33–40. [In Russian]
Mollaeva MZ, Tembotova FA (2022) Quality and anomalies of Scots pine pollen in the Central Caucasus. News of the Russian Academy of Sciences. Biological series 3: 272–278. https://doi.org/10.1134/S1062359022030128 [In Russian]
Monoszon–Smolina MH (1949) On the question of pollen morphology of some species of the genus Pinus. Botanical journal 4: 352–380. [In Russian]
Noskova NE, Tret'jakova IN (2006) Evaluation of stress on reproductive performance of Scots pine. Conifers of the boreal zone 1: 54–64. [In Russian]
Pausheva ZP (1988) Practical training in plant cytology. Forth Edition. Agropromizdat, Moscow, 272 pp. [In Russian]
Reshetova SA (2019) Pollen of plants of urbanized territories as a bioindicator of conditions of technogenic pollution (Transbaikalia). Geosphere research 4: 15–23. http://dx.doi.org/10.17223/25421379/13/2 [In Russian]
Şeker M, Erdoğan A, Korkmaz S, Küplülü Ö (2022) Bee pollens as biological indicators: An ecological assessment of pollution in Northern Turkey via ICP-MS and XPS analyses. Environmental Science and Pollution Research 29: 36161–36169. https://doi.org/10.1007/s11356-021-18007-y
Shengnan Zhao, Yinhui Ren, Cunxu Wei (2022) Staining Starch with iodine Solution. In: Pellicciari C, Biggiogera M, Malatesta M (Eds) Histochemistry of Single Molecules. Methods in Molecular Biology 2566: 281–290. https://doi.org/10.1007/978-1-0716-2675-7_23
Tret'jakova IN, Noskova NE (2004) Scots pine pollen under environmental stress. Ecology 3: 26–33. https://doi.org/10.1023/B:RUSE.0000011105.90297.07 [In Russian]
Tret'jakova IN, Zubareva ON, Bazhina EV (1996) The influence of environmental pollution with sulfur oxides on the crown morphostructure, generative sphere and pollen viability of Siberian fir in the Baikal region. Ecology 1: 17–23. [In Russian]
Valetova EA (2009) The impact of technogenic pollution on the reproductive capacity of Scots pine. Abstract of a dissertation for the degree of candidate of biological sciences. Barnaul, 19 pp. [In Russian]
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