STREPTOMYCES AVERMITILIS: COMPONENT COMPOSITION, PROPERTIES
UDC 579.873.71, 582.29
Abstract
The component composition of S. avermitilis biomass was studied, including avermectin, lipids, proteins, and a polysaccharide complex (murein). It was established that the composition of lipids of S. avermitilis contains predominantly saturated fatty acids – 86%, the content of monounsaturated (palmitic and vaccine) and polyunsaturated (linoleic and α-linolenic) fatty acids is the same and is about 7%. It should be noted the presence of a significant amount of branched chain fatty acids (isoforms) – more than 70% of the total amount of fatty acids. A technique has been developed for isolating the polysaccharide complex from S. avermitilis biomass. Using IR spectroscopy, it was found that the S. avermitilis polysaccharide complex, as well as the chitin-glucan complex isolated from the thallus of the epigeneic lichen of the species Cladonia rangiferina, is built from chitin units – β-N-acetylglucosamine. It was shown that the polysaccharide complex (murein) has a polyampholytic nature and has high sorption properties with respect to the main (methylene blue) and acid (Congo red) dyes. It was also established that the sorption capacity with respect to the dyes under study for the polysaccharide complex S. avermitilis is 2.8–7.6 times higher than for the chitin – glucan complex Cladonia rangiferina. This indicates a significantly higher content of active functional groups (sorption centers) in the murein structure, which allows us to recommend it for use as an effective sorbent (enterosorbent).
Downloads
Metrics
References
Watve M.G., Tickoo R., Jog M.M., Bhole B.D. Arch. Microbiol., 2001, vol. 176, pр. 386–390 DOI: 10.1007/s002030100345.
Burg R.W., Miller B.M., Baker E.E. et al. Antimicrob. Agents and Chemother, 1979, no. 15, pр. 361–367.
Belyavskaya L.A., Galagan T.A., Boltovskaya Ye.V., Kozyritskaya V.Ye., Valagurova Ye.V., Sigareva D.D., Iutin-skaya G.A. Stiinta Agricola, 2009, no. 1, pp. 29–33 (in Russ.).
Maksimov I.V. Izvestiya Ufimskogo nauchnogo tsentra RAN, 2013, no. 2, pp. 38–61. (in Russ.).
Sherstnev V.V. Vliyaniye na immunologicheskiy status sviney preparata niatsid, poluchayemogo po tekhnologiche-skoy skheme, oriyentirovannoy na bezotkhodnoye proizvodstvo: dis. … kand. biol. nauk. [The effect on the immunological status of pigs of the drug niacid obtained by the technological scheme, focused on waste-free production: dis. ... cand. biol. sciences]. Moscow, 2002, 110 p. (in Russ.).
Kurchenko V.P., Buga S.V., Petrashkevich N.V., Butkevich T.V. i dr. Trudy BGU, 2016, vol. 11, no. 1, pp. 110–126 (in Russ.).
Lipke P.N., Ovalle R. J. of Bacteriology, 1998, vol. 180, no. 15, pр. 3735–3740.
Muzzarelli R.A.A., Tanfani F.E.M. J. Appl. Biochem., 1981, vol. 3, no. 4, pр. 322–327.
Muzzarelli R.A.A., Tanfani F.E.M. Proc. 2nd Int. conf. «Chitin and chitosan», Sapporo, 1982, pр. 183–186.
Zakharova N.G., Vershinina V.I., Il'inskaya O.N. Kratkiy kurs po mikrobiologii, virusologii i immunologii. [Short course in microbiology, virology and immunology]. Kazan', 2015, 799 p. (in Russ.).
Gusev M.V., Mineyeva L.A. Mikrobiologiya. [Microbiology]. Moscow, 1992, 448 p. (in Russ.).
Ivshin V.P., Artamonova S.D., Ivshina T.N., Sharnina F.F. Vysokomolekulyarnyye soyedineniya. Seriya B, 2007, vol. 49, no. 12, pp. 2215–2222 (in Russ.).
Unrod V.I., Solodovnik T.V. Biopolimery i kletka, 2001, vol. 17, no. 6, pp. 526–533 (in Russ.).
Marguerite R. Prog. Polym. Sci., 2006, pр. 603–632. DOI: 10.1016/j.progpolymsci.2006.06.001.
Gosudarstvennaya Farmakopeya SSSR: Vyp. 1. Obshchiye metody analiza MZ SSSR. [State Pharmacopoeia of the USSR: Issue. 1. General methods of analysis of the Ministry of Health of the USSR]. Moscow, 1987, pp. 285–286 (in Russ.).
Pavlova O.V., Belova Ye.A., Trotskaya T.P. Nauchnyye trudy odesskoy natsional'noy akademii pishchevykh tekhnologiy, 2012, vol. 2, no. 46, pp. 121–124 (in Russ.).
Burtseva S.A. Míkrobíol. zhurn., 2000, vol. 62, no. 3, pp. 9–16 (in Russ.).
Feofilova Ye.P. Immunopatologiya, allergologiya, infektologiya, 2004, no. 1, pp. 27–32 (in Russ.).
Lipidy: Struktura, biosintez, prevrashcheniya i funktsii. [Lipids: Structure, biosynthesis, transformations and functions]. Moscow, 1987, 182 p. (in Russ.).
Koronelli T.V. Lipidy mikrobakteriy i rodstvennykh mikroorganizmov. [Lipids of microbacteria and related microorgan-isms]. Moscow, 1984, 156 p. (in Russ.).
Donets A.T. Izucheniye biosinteza lipidov u nekotorykh saprofitnykh mikrobakteriy: avtoref. dis. … kand. biol. nauk. [The study of lipid biosynthesis in some saprophytic microbacteria: author. dis. ... cand. biol. sciences]. Saratov, 1971, 23 p. (in Russ.).
Budnikov G.K. Khimicheskiy analiz v meditsinskoy diagnostike. [Chemical analysis in medical diagnostics]. Moscow, 2010, 504 p. (in Russ.).
Greg S., Sing K. Adsorbtsiya, udel'naya poverkhnost', poristost'. [Adsorption, specific surface area, porosity]. Moscow, 1984, 306 p. (in Russ.).
Zhil'tsov D.V., Brovko O.S., Ivakhnov A.D., Bogolitsyn K.G., Boytsova T.A., Palamarchuk I.A. Uspekhi sovremennogo yestestvoznaniya, 2018, no. 11, pp. 210–215 (in Russ.).
Zhil'tsov D.V., Brovko O.S., Palamarchuk I.A., Boytsova T.A., Bogolitsyn K.G., Chukhchin D.G. Izvestiya Ufimskogo nauchnogo tsentra RAN, 2018, no. 3(3), pp. 9–13. DOI: 10.31040/2222-8349-2018-3-3-8-12 (in Russ.).
Copyright (c) 2020 Khimiya Rastitel'nogo Syr'ya (Chemistry of plant raw material)

This work is licensed under a Creative Commons Attribution 4.0 International License.

This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.







