EVALUATION OF METHODS FOR DETERMINATION OF ANTIOXIDANT POTENTIAL OF WATER-ETHANOL EXTRACTS OF R. ACICULARIS
UDC 581.1
Abstract
Oxidative stress, which underlies the pathogenesis of more than 200 diseases, actualizes the search for natural antioxidants. Prickly rose (Rosa acicularis Lindl.), which grows in extreme conditions, is of interest due to its high content of biologically active substances. However, its antioxidant potential has not been studied sufficiently compared to other species of the genus Rosa. In this work, a comprehensive evaluation of the antioxidant activity of water-ethanol extracts of leaves, fruit pulp, and seeds of R. acicularis collected in Yakutia was carried out using the following methods: Fe2+-induced peroxidation of yolk lipoproteins, DPPH assay, and chemiluminescence. The phytochemical profile of extracts from different plant organs was found to vary significantly: leaves are rich in phenols (140.99 mg/g) and flavonoids (2.51 mg/g), fruit pulp in ascorbic (40.71 mg/g) and gallic acids (4.35 mg/g), and seeds in phenylpropanoids (1.51 mg/g). The antioxidant activity of the extracts showed dependence on the method of analysis: leaf extracts were effective in radical-binding tests (DPPH), fruit pulp extracts were effective in metal chelation (Fe2+-model), and seeds were inactive. The results emphasize the need to combine methods for an objective evaluation that considers the mechanisms of action of antioxidants and the specificity of the plants. The study confirms the promise of R. acicularis as a source of natural antioxidants for medicine and nutraceuticals and the importance of standardizing approaches for analyzing bioactive extracts.
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