QUANTITATIVE DETERMINATION OF THE SUM OF FLAVONOIDS OF THE ABOVEGROUND PART OF SCUTELLARIA COMOSA

UDC 615.322.581.8+44+45:582.949.2

  • Munirakhon Akhmathon kizi Mamatkhanova Institute of Chemistry of Plant Substances, AS RUz Email: munir_05@mail.ru
  • Shohida Abdukodir kizi Ergasheva Institute of Chemistry of Plant Substances, AS RUz Email: ergashev1017@mail.ru
  • Erkin Khozhiakbarovich Botirov Institute of Chemistry of Plant Substances, AS RUz; Surgut State University Email: botirov-nepi@mail.ru
  • Maksim Aleksandrovich Mulyukin Surgut State University Email: 16-maks-97@mail.ru
  • Ravshanjon Muratjanovich Khalilov Institute of Chemistry of Plant Substances, AS RUz Email: r.m.khalilov@mail.ru
  • Akhmatkhon Umarkhanovich Mamatkhanov Institute of Chemistry of Plant Substances, AS RUz Email: prof.ahmad@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20230111301
Keywords: Scutellaria comosa, aboveground, flavonoids, isoscutellarein 7-O-β-D-glucopyranoside, spectrophotometry

Abstract

As a result of pharmacological studies, it was revealed that the sum of flavonoids from the aboveground part of the plant Scutellaria comosa (Lamiaceae family) has adaptogenic and antihypoxic effects. For the introduction into medical practice of a drug based on the sum of flavonoids, studies have been conducted on the standardization of raw materials – the aboveground part of the crested helmet. The analysis of plant raw materials by TLC and HPLC methods showed that the dominant flavonoid of the aboveground part of the crested skullcap is isoscutellarein 7-O-β-D-glucopyranoside.

A differential spectrophotometric technique has been developed for the quantitative determination of the sum of flavonoids of the aboveground part of the crested helmet in terms of isoscutellarein 7-O-β-D-glucopyranoside. The optimal parameters for the extraction of vegetable raw materials are: extraction with 70% ethyl alcohol in a boiling water bath for 60 minutes in the ratio "raw material – extractant" – 1 : 100, the degree of grinding of raw materials – 2 mm. It was found that with the differential version of spectrophotometric analysis, the flavonoids – AlCl3 system reaches equilibrium after 40 minutes. The maximum of the differential absorption spectrum of the alcohol extraction of Scutellaria comosa is observed at 346 nm and can be used to analyze this group of compounds.

The content of the sum of flavonoids in the aboveground part of the crested helmet varies from 9.72% to 10.27%. In order to establish the optimal harvesting time of plant raw materials, the content of the sum of flavonoids was determined by the vegetation periods of the plant in terms of isoscutellarein 7-O-β-D-glucopyranoside and it was determined that the maximum accumulation of the sum of flavonoids is observed during the flowering period (10.13% of the weight of air-dry raw materials).

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Author Biographies

Munirakhon Akhmathon kizi Mamatkhanova, Institute of Chemistry of Plant Substances, AS RUz

кандидат технических наук, старший научный сотрудник экспериментально-технологической лаборатории

Shohida Abdukodir kizi Ergasheva, Institute of Chemistry of Plant Substances, AS RUz

базовый докторант экспериментально-технологической лаборатории

Erkin Khozhiakbarovich Botirov, Institute of Chemistry of Plant Substances, AS RUz; Surgut State University

доктор химических наук, профессор, заведующий лабораторией химии терпеноидов и фенольных соединений

Maksim Aleksandrovich Mulyukin, Surgut State University

аспирант

Ravshanjon Muratjanovich Khalilov, Institute of Chemistry of Plant Substances, AS RUz

доктор технических наук, ведущий научный сотрудник экспериментально-технологической лаборатории

Akhmatkhon Umarkhanovich Mamatkhanov, Institute of Chemistry of Plant Substances, AS RUz

доктор технических наук, профессор, ведущий научный сотрудник экспериментально-технологической лаборатории

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Published
2023-03-13
How to Cite
1. Mamatkhanova M. A. kizi, Ergasheva S. A. kizi, Botirov E. K., Mulyukin M. A., Khalilov R. M., Mamatkhanov A. U. QUANTITATIVE DETERMINATION OF THE SUM OF FLAVONOIDS OF THE ABOVEGROUND PART OF SCUTELLARIA COMOSA // chemistry of plant raw material, 2023. № 1. P. 239-246. URL: http://journal.asu.ru/cw/article/view/11301.
Issue
No 1 (2023)
Section
Low-molecular weight compounds

Copyright (c) 2023 chemistry of plant raw material

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