STUDY OF THE CONTENT OF POLYPHENOLS AND ANTIOXIDANT ACTIVITY OF THE EXTRACT OF GREEN MARINE ALGAE ULVA LACTUCA L.
UDC 615.322+577.121
Abstract
It was studied the impact of a water-alcohol extract (70%) from the marine green algae Ulva lactuca L., enriched with a polyphenol fraction, and a herbal preparation – extract of Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (Araliaceae) on the indicators of antioxidant protection of the liver and blood plasma of rats under acute stress (vertical fixation by the dorsal neck fold). The total polyphenols and flavonoids content in the extract of U. lactuca made 16.2±1.8 µg GAE/g and 9.10±1.87 g QE/g, respectively, which was 2 times higher than its amount in the Eleutherococcus extract – 7.6±0.25 µg GAE/g and 4.8±0.3 µg QE/g respectively. The antiradical activity against ABTS+ and alkyl-peroxyl radicals of the U. lactuca extract was also almost 2 times higher in compere with Eleutherococcus. The pharmacological effect of the phenolic complex of U. lactuca in Wistar rats under stress was manifested in the stabilization of the antioxidant defense system. It was noted a decrease of the malondialdehyde content, an increase of the antiradical activity level, reduced glutathione content and the activity of enzymes of glutathione cycle in the liver and blood plasma of rats. In terms of efficiency, the extract of U. lactuca was not inferior to the reference preparation Eleutherococcus, and in terms of the ability to restore the activity of antioxidant enzymes and indicators of the glutathione system, it was superior to that. The pharmacological effect of the phenolic complex of U. lactuca, to our opinion, is provided by the action of its constituent flavonoids, phenolic terpenoids, phenolic acids (hydroxybenzoic, hydroxycinnamic, coumaric, synapic), etc., which have pronounced antioxidant properties, which prevents the development of lipid peroxidation and oxidative stress. Thus, the marine green algae U. lactuca is a promising raw material for the development of preparations that can increase the potential of the endogenous antioxidant defense system of the body at stress-induced disorders.
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Tian C., Hao L., Yi W., Ding S., Xu F. Oxidative Medicine and Cellular Longevity, 2020, pp. 1–2. DOI: 10.1155/2020/7398453.
Manach C., Scalbert A., Morand C., Remesy C., Jimenez L. American Journal of Clinical Nutrition, 2004, vol. 79, no. 5, pp. 727–747. DOI: 10.1093/ajcn/79.5.727.
Cotas J., Leandro A., Monteiro P., Pacheco D., Figueirinha A., Goncalves A.M.M., da Silva G.J., Pereira L. Marine Drugs, 2020, vol. 18, no. 8, pp. 384–431. DOI: 10.3390/md18080384.
Farasat M., Khavari-Nejad R.A., Nabavi S.M., Namjooyan F. Iranian Journal of Pharmaceutical Research, 2014, vol. 13, no. 1, pp. 163–170.
Cho M., Kang I.J., Won M.H., Lee H.S., You S. Journal of Medicinal Food, 2010, vol. 13, no. 5, pp. 1232–1239. DOI: 10.1089/jmf.2010.1124.
Alagan V.T., Valsala R.N., Rajesh K.D. British Journal of Pharmaceutical Research, 2017, vol. 15, no. 1, pp. 1–14. DOI: 10.3923/ijp.2018.572.583.
Zhong B., Robinson N.A., Warner R.D., Frank R. Marine Drugs, 2020, vol. 18, no. 6, pp. 331–352. DOI: 10.3390/md18060331.
Leopoldini M., Russo N., Toscano M. Food Chem., 2011, vol. 125, pp. 288–306. DOI: 10.1016/j.foodchem.2010.08.012.
Sprygin V.G., Fomenko S.Ye., Kushnerova N.F. Fundamental'nyye issledovaniya, 2014, no. 8-1, pp. 110–114. (in Russ.).
Fomenko S.Ye., Kushnerova N.F., Sprygin V.G., Drugova Ye.S., Merzlyakov V.Yu., Lesnikova L.N Khimiya Rastitel'nogo Syr'ya, 2019, no. 3, pp. 41–51. DOI: 10.14258/jcprm.2019035116. (in Russ.).
Kushnerova N.F., Fomenko S.Ye., Sprygin V.G., Drugova Ye.S., Momot T.V., Merzlyakov V.Yu., Lesnikova L.N. Biologiya morya, 2022, vol. 48, no. 2, pp. 123–132. DOI: 10.31857/S0134347522020073. (in Russ.).
Valko M., Leibfritz D., Moncol J., Cronin M.T., Mazur M., Telser J. The International Journal of Biochemistry & Cell Biology, 2007, vol. 39, pp. 44–84. DOI: 10.1016/j.biocel.2006.07.001.
Chrousos G.P. Nature Reviews Endocrinology, 2009, no. 5, pp. 374–381. DOI: 10.1038/nrendo.2009.106.
Titlyanov E.A., Titlyanova T.V. Morskiye rasteniya stran Aziatsko-Tikhookeanskogo regiona, ikh ispol'zovaniye i kul'tivirovaniye. [Marine plants of the countries of the Asia-Pacific region, their use and cultivation]. Vladivostok, 2012, 377 p. (in Russ.).
Sprygin V.G., Kushnerova N.F., Fomenko S.Ye., Sizova L.A., Momot T.V. Biologiya morya, 2013, vol. 39, no. 1, pp. 50–54. (in Russ.).
Parys S., Rosenbaum A., Kehraus S., Reher G., Glombitza K.W., König G.M. Journal of Natural Products, 2007, vol. 70, no. 12, pp. 1865–1870. DOI: 10.1021/np070302f.
Chan P.T., Matanjun P., Yasir S.M., Tan T.S. Journal of Applied Phycology, 2015, vol. 27, no. 6, pp. 2377–2386. DOI: 10.1007/s10811-014-0493-1.
Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C. Free Radical Biology and Medicine, 1999, vol. 26, no. 9-10, pp. 1231–1237. DOI: 10.1016/s0891-5849(98)00315-3.
Bartosz G., Janaszewska A., Ertel D., Bartosz M. Biochemistry and Molecular Biology International, 1998, vol. 46, no. 3, pp. 519–528. DOI: 10.1080/15216549800204042.
Fomenko S.Ye., Kushnerova N.F., Sprygin V.G., Momot T.V. Tikhookeanskiy meditsinskiy zhurnal, 2013, no. 2, pp. 67–70. (in Russ.).
Vengerovskiy A.I., Markova I.V., Saratikov A.S. Vedomosti farmakologicheskogo komiteta, 1999, no. 2, pp. 9–12. (in Russ.).
Davydov M., Krikorian A.D. Journal of Ethnopharmacology, 2000, vol. 72, pp. 345–393
Buege J.A., Aust S.D. Methods Enzymology, 1978, vol. 52, pp. 302–310.
Paoletti F., Aldinucci D., Mocali A., Caparrini A. Analytical biochemistry, 1986, vol. 154, no. 2, pp. 536–541. DOI: 10.1016/0003-2697(86)90026-6.
Goldberg D.M., Spoone R.J. Methods of enzymatic analysis. Deerfield Beach, 1983, vol. 3, pp. 258–265.
Burk R.F., Lawrence R. A., Lane J.M. Journal of Clinical Investigation, 1980, vol. 65, no. 5, pp. 1024–1031.
Karpishchenko A.I., Alipov A.N., Alekseyev V. V. Meditsinskiye laboratornyye tekhnologii. Rukovodstvo po klinich-eskoy laboratornoy diagnostike. [Medical laboratory technologies. Guidelines for Clinical Laboratory Diagnostics]. Moscow, 2013, vol. 2, 792 p. (in Russ.).
Adamczyk K., Kuzniewski R., Olech V., Rapacka-Gackowska A., Abramek J., Bogucka-Kocka A., Wojciak-Kosior M., Skalski T., Feldo M., Pietrzak W., Nowak R., Strzemski M., Zaluski D. Oxidative Medicine and Cellular Longevity, 2019, pp. 1–10. DOI: 10.1155/2019/8673521.
Kurkin V.A., Ryazanova T.K. Khimiko-farmatsevticheskiy zhurnal, 2022, vol. 56, no. 3, pp. 34–41, DOI: 10.30906/0023-1134-2022-56-3-34-41. (in Russ.).
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