ISOLATION OF DIHYDROQUERCETIN AND ARABINOGALACTAN FROM LARCH WOOD WITH WATER-ETHANOL SOLUTIONS

UDC 54.05

  • Vladimir Aleksandrovich Levdansky Institute of Chemistry and Chemical Technology SB RAS, FRC KSC SB RAS Email: vlevdanskij@mail.ru
  • Aleksandr Vladimirovich Levdansky Institute of Chemistry and Chemical Technology SB RAS, FRC KSC SB RAS Email: alexsander.l@mail.ru
  • Boris Nikolayevich Kuznetsov Institute of Chemistry and Chemical Technology SB RAS, FRC KSC SB RAS Email: bnk@icct.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20220411959
Keywords: larch wood, mechanical activation, extraction, dihydroquercetin, arabinogalactan

Abstract

Siberian larch wood (Larix sibirica Lebed.) contains such valuable biologically active substances as dihydroquercetin (DHQ) and arabinogalactan (AG), promising for the use in the pharmaceutical and food industries. An urgent task is to improve the methods of extraction isolation of these compounds from wood. In this work, we studied the possibility of simultaneous isolation of dihydroquercetin and arabinogalactan by extraction of larch wood with 5–25% aqueous ethanol solutions. It has been shown that by extracting wood with aqueous solutions with a low concentration of ethanol, it is possible to exclude the stage of separation of resinous substances from the target products of DHQ and AG. By experimentally optimizing of water-ethanol extraction process, it was shown that the highest yields of DHQ (1.8% mas.) and AG (18.0% mas.) were achieved by extraction of larch wood, crushed to particles of 1–3 mm in size, with a 15% ethanol solution for 2 hours. That the preliminarily mechanical treatment of larch wood allows to reduce the time of water-alcohol extraction to 30 minutes and to obtain DHQ and AG with high yields. The structure of DHQ and AG was confirmed by FTIR and NMR spectroscopy. Identification and purity of DHQ isolated from larch wood were confirmed by a photometric method using the reaction of cyanidin chloride formation when DHQ in heated in ethanol in the presence of hydrochloric acid.

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Author Biographies

Vladimir Aleksandrovich Levdansky, Institute of Chemistry and Chemical Technology SB RAS, FRC KSC SB RAS

доктор химических наук, ведущий научный сотрудник

Aleksandr Vladimirovich Levdansky, Institute of Chemistry and Chemical Technology SB RAS, FRC KSC SB RAS

кандидат химических наук, научный сотрудник

Boris Nikolayevich Kuznetsov, Institute of Chemistry and Chemical Technology SB RAS, FRC KSC SB RAS

доктор химических наук, профессор, заведующий лабораторией химии природного органического сырья, профессор кафедры аналитической и органической химии

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Published
2022-12-15
How to Cite
1. Levdansky V. A., Levdansky A. V., Kuznetsov B. N. ISOLATION OF DIHYDROQUERCETIN AND ARABINOGALACTAN FROM LARCH WOOD WITH WATER-ETHANOL SOLUTIONS // chemistry of plant raw material, 2022. № 4. P. 107-113. URL: http://journal.asu.ru/cw/article/view/11959.
Issue
No 4 (2022)
Section
Biopolymers of plants

Copyright (c) 2022 chemistry of plant raw material

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