PREPARATIVE ISOLATION OF HYDROLYZABLE TANNINS FROM LEAVES OF CORNUS SERICEA L. USING TELLIMAGRANDIN I AS AN EXAMPLE
UDK 615.322
Abstract
Hydrolysable tannins are a promising class of natural compounds for medical applications. They have found many pharmacological activities, the most promising of which is antiviral. The difficulty of obtaining pure tannins is one of the main problems in their study. A complex mixture of high molecular weight homologues and isomers is obtained as a result of the biosynthesis of tannins. Some of the tannins additionally complicate the preparative separation significantly because they have a non-standard broad peak shape, which contaminates the nearest peaks when collecting eluates. The rapid change in conformation on the chromatographic column and in solution is the reason.
The paper describes a complex method for the isolation of hydrolyzable tannins from the leaves of Cornus sericea L. The method includes obtaining an extract from plant materials, its fractionation on a Sephadex LH-20 column, and isolation of individual hydrolyzable tannins using preparative high-performance liquid chromatography with a UV detector on a Silasorb C18 column. The second stage includes purification on a Luna® C18 column. Ultra-performance liquid chromatography with diode and mass spectrometric detector and 1H-NMR spectroscopy were used to identify the isolated hydrolysable tannins and evaluate their purity. Using isolated tellimagrandin I as an example, the features of the chromatographic behavior of hydrolysable tannins with a free hydroxyl group at the anomeric carbon atom of glucose are shown. This type of substances have two isomers and simultaneously exist in α- and β-forms.
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