METHOD OF QUANTITATIVE DETERMINATION OF THE AMOUNT OF ANTHOCYANINS IN THE FRUITS OF MALPIGHIA EMARGINATA
UDC 615.322:543.422.3
Abstract
The objects of the study were mature dried fruits of the fringed malpighia (Malpighia Emarginata, fam. Malpighiaceae). The fruits of malpighia are widely used in foreign medical practice as an antioxidant. The fruits of malpighia contain anthocyanins, vitamins, carotenoids. The most common method of quantitative determination of anthocyanins is spectrophotometry. The purpose of this work is to determine the amount of anthocyanins in the fruits of malpighia by spectrophotometry.
Quantitative determination of the amount of anthocyanins in the fruits of malpighia by direct spectrophotometry was carried out. The values of the optical density of the anthocyanin solution were recorded at a wavelength in the region of 250–700 nm. The analytical maxima of the studied compounds at 534 nm characteristic of cyanidin-3-O-glucoside were determined. Optimal conditions for the extraction of anthocyanins from the raw materials of this plant were determined (extractant – 40% ethanol; the ratio of "raw material – extractant" – 1 : 50; extraction time – 30 minutes; the degree of grinding of raw materials – 1.0 mm). It was determined that the error of a single determination of the content of anthocyanins in the fruits of malpighia with a confidence probability of 95% is ±6.79%.
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