QUANTITATIVE DETERMINATION OF SAPONINS IN THE DRUGS OF ARALIA ELATA
Abstract
The method of the quantitative determination of the content of total saponins in the herbal drugs (tincture and syrup) of Aralia elata (Miq.) Seem. (synonym: Aralia mandshurica Rupr. et Maxim.) was developed by means of the direct spectrophotometry following reaction with concentrated sulfuric acid (analytical wavelength – 510 nm for tincture and 525 nm for syrups). The quantitative determination of the content of total saponins in syrups was conducted following preliminary hydrolysis in the presence of 20% sulfuric acid during two hours. The content of total saponins in the tincture varies from 1.51±0.05% to 1.72±0.06% calculated on ammonium salt of aralosides A, B, C with average molecular weight, in syrups from 0.131±0.003 % to 0.145±0.004 % calculated on oleanolic acid (0.287-0.318% calculated on ammonium salt of aralosides A, B, C with average molecular weight). It was determined that the error of a single definition of the contents of total saponins in the tincture from the roots of Aralia mandshurica with confidence probability of 95 % is + 3.14 %, in syrup from “Saparal” ±2.62%, in syrup from tincture ±3.17%.
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