VALIDATION EVALUATION METHODS FOR DETERMINING RUTIN IN LEAVES OF WILLOW TRIANDRA BY METHOD PLANAR CHROMATOGRAPHY
Abstract
Presents a validated method of identification and quantitative determination of rutin in the leaves triandra willow (Salix triandra L.) by planar thin-layer chromatography with densitometric registration of the analytical signal. For the experiment used an ethanolic extract (ethanol 70%) from the dried, previously defatted with chloroform leaf willow.
Digital processing of the chromatograms was performed by using the computer program "Sorbfil Videodensitometer" (Krasnodar). The technique is validated by the following characteristics: specificity, sensitivity – limit of detection, the limit of quantification , linearity, trueness, precision.
Specificity – the color and rf of the spots of the control track corresponds to the color and Rf of the spots of the standard sample (0,63±0,02).Sensitivity: limit of detection = 0,8 µg; limit of quantification = 2,4 µg. Linearity - established linear relationship between amount of analyte and analytical signal – the regression equation has the form: S = 3,84·103 v; correlation coefficient r = 0,997. Trueness – 99,5±5,1%
Determined the content of rutin in leaves of willow trentacinque, cultivated in the conditions of the Caucasian Mineral Waters.This technique is recommended for studies of chemical composition and development of quality standards for medicinal raw materials.Downloads
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