AGAR/NACMC COMPOSITE IS AN EFFECTIVE MEDIUM FOR THE GROWTH OF MICROORGANISMS

UDC 678.555

  • Iraida Ivanovna Osovskaya Saint Petersburg State University of Industrial Technologies and Design, Higher School of Technology and Power Engineering https://orcid.org/0009-0006-5771-7784 Email: iraosov@mail.ru
  • Anna Evgenievna Baranova Saint Petersburg State University of Industrial Technologies and Design, Higher School of Technology and Power Engineering Email: barya9309@mail.ru
  • Aleksandr Vyacheslavovich Kurzin Saint Petersburg State University of Industrial Technologies and Design, Higher School of Technology and Power Engineering Email: zakora@mail.ru
  • Viktor Ivanovich Saint Petersburg State Forest Engineering University named after S.M. Kirov Email: kaf.chemdrev@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20240313610
Keywords: agar, gelation, Agar/Na-CMC composite, IR spectroscopy, microorganism growth

Abstract

A method for producing an agar/sodium carboxymethylcellulose composite has been developed for using it in microbiological practice. The physicochemical and colloidal-chemical properties of the obtained composite (surface tension, solubility, swelling, microscopy) have been studied. The gel-forming and film-forming abilities of the composite have been studied at different component ratios. IR spectra of composite films have been measured at different ratios of agar and sodium carboxymethylcellulose to characterize the nature of chemical bonds. The studied composite has been used as a nutrient medium in microbiology. The conditions for creating the nutrient medium have been optimized. The surface cultivation method has been used. The optimal time and temperature for growing microorganisms have been revealed. The ratio of polymer components in the nutrient medium has been determined. Images of microorganisms have been obtainned on an MBR-3 biological research microscope with a resolution of 100 times during the entire cultivation period. Microorganism growth was controlled by the OpenCFU program for colony-forming unit counting. The maximum number of colonies grown corresponds to the agar/sodium carboxymethyl cellulose ratio of 3 : 1. The expediency of using the composite for culturing bacterial cells in vitro is shown. The results of this study can be recommended for use in the food, textile, pulp and paper industries as a stabilizer, emulsifier, and polyampholyte with high sorption capacity.

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Author Biographies

Iraida Ivanovna Osovskaya , Saint Petersburg State University of Industrial Technologies and Design, Higher School of Technology and Power Engineering

candidate of chemical sciences, associate professor, associate professor of the Department of Physical and Colloid Chemistry

Anna Evgenievna Baranova , Saint Petersburg State University of Industrial Technologies and Design, Higher School of Technology and Power Engineering

master's student

Aleksandr Vyacheslavovich Kurzin , Saint Petersburg State University of Industrial Technologies and Design, Higher School of Technology and Power Engineering

candidate of chemical sciences, associate professor, associate professor of the Department of Organic Chemistry

Viktor Ivanovich , Saint Petersburg State Forest Engineering University named after S.M. Kirov

doctor of chemical sciences, head of the Department of Technology of Forest Chemical Products, Wood Chemistry and Biotechnology

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Published
2024-09-24
How to Cite
1. Osovskaya I. I., Baranova A. E., Kurzin A. V., Viktor Ivanovich AGAR/NACMC COMPOSITE IS AN EFFECTIVE MEDIUM FOR THE GROWTH OF MICROORGANISMS // chemistry of plant raw material, 2024. № 3. P. 71-80. URL: http://journal.asu.ru/cw/article/view/13610.
Issue
No 3 (2024)
Section
Biopolymers of plants

Copyright (c) 2024 chemistry of plant raw material

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