STUDY OF PHENOLIC COMPOUNDS IN ABOVEGROUND PART OF LAVATERA THURINGIACA, GROWING IN THE ALTAI KRAI
Abstract
Lavatera thuringiaca L. family Malvaceae (Malvaceae) contains a rich complex of biologically active compounds and is widely used in folk medicine as an anti-inflammatory, enveloping, expectorant agent. The goal of this research is to study of phenolic compounds in aboveground part of Lavatera thuringiaca, growing in the Altai Region. To achieve the goal needs to solve the following problem: conducting qualitative reactions to flavonoids, separation and identification of phenolic compounds by TLC and HPLC.
The results of qualitative reactions allowed to sugges the presence in the raw material of flavonoids groups flavonol, flavone, flavanone. Using the method of thin layer chromatography in the system ethyl acetate - acetic acid - water (70:10:20) detected flavonoids flavone and flavonol groups. Then prepared ethanolic extraction and separation was divided into fractions: ether, ethylacetate and butanolic. By HPLC in the ester fraction were identified caffeic acid, coumaric derivatives and ferulic acid, flavonoids of flavone, trimethoxyflavone, isoflavone groups, chalcone derivative; in ethyl acetate fraction - caffeic acid, gallic derivatives and ferulic acids, flavonoids of flavone, trimethoxyflavone groups; in butanol fraction - flavonoid of group flavone, apigenin and catechol derivatives.
The research aimed at studying the possibility of applying the aboveground part of the Lavatera thuringiaca in medicine. It is planned to develop standard documentation on medicinal plant raw material.
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