HISTOCHEMICAL STUDY XYLEM CELLS HAVE IRIS SIBIRICA L. IN CULTURE IN VITRO
Abstract
This paper presents the data content of the lignin from annual plants-regenerants Iris sibirica is comparable to the content of a 6-year-old intact plants. With the aim of identifying conditions of accelerated lignification studied the structure of the shoots of Iris sibirica , grown on artificial nutrient media, using the methods of histochemistry.
Peculiarities of the formation of the xylem from Iris sibirica on artificial nutrient media. Very quickly regenerants developed a complex system consisting of conducting bundles containing sieve tubes, vessels and tracheids, and as a network of gidrozit. Hydrocity Iris sibirica in its structure is tracheids with significiance the swelling, but, in contrast to tracheids and vessels of xylem (they are formed on the basis of procube or cambium – lateral special primary or secondary meristem), hydrocity differentiated from the cells of permanent tissues (like phellogen), which probably at the time of differentiation possessed meristematic activity. From Iris sibirica hydrocity thick layer envelops the conductive beam and accompanied him along the escape at a certain height. Due to the formation of the thick fabric of significiance tracheal elements, young regenerants Iris sibirica was high lignin content.
The study of the differentiation of xylem elements in the laboratory can serve as a model for our understanding of the processes of formation of wood.
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