THE USE OF EXTRACTS OF DAHLIA, ROSE AND PEONY FLOWERS FOR HISTOLOGICAL STAINING
Abstract
In a series of previous studies a new group of plant dyes was developed for histology on the basis of the fruit extracts containing anthocyanin pigments. In the present study the tinctorial properties of ethanol extracts of dahlia (Dahlia Cav.), rose (Rosa L.) and peony (Paeonia L.) petals which also contain anthocyanins were evaluated. The choice of the plant group mentioned above was based on the availability of vegetable raw materials, the large size of the petals for the easy pickup and on the red color associated with the presence of anthocyanins. On the basis of ethanol extracts of dahlias’ petals the dye for staining both the cellular nuclei and the cytoplasm was obtained. When using the petals of rose and peony flowers cytoplasmic histological dyes were received. All new dyes belong to the group of mordant stains, since their staining properties for histological slides are expressed when using iron sulphate that reacts with vegetable pigments. With the help of comparative analysis it was found for the first time that vegetable dyes are able to stain the cell nuclei and basophilic structures of the cytoplasm if they contain predominantly the monoglycoside varieties of anthocyanins. In the presence of 3,5-diglucoside anthocyanins the dyes demonstrate the cytoplasmic nature.
Downloads
Metrics
References
Korzhevskii D.E. Morfologiia, 2007, vol. 132, no. 6, pp. 77–82. (in Russ.).
Ishunina T.A., Solonenko O.V. Morfologicheskie vedomosti, 2014, no. 4, pp. 58–61. (in Russ.).
Ishunina T.A. Morfologicheskie vedomosti, 2015, no. 4, pp. 65–68. (in Russ.).
Ishunina T.A. Struktura i fiziko-khimicheskie svoistva tselliuloz i nanokompozitov na ikh osnove. [Structure and physi-co-chemical properties of celluloses and nanocomposites based on them]. Petrozavodsk, 2016, pp. 111–115. (in Russ.).
Horbowicz M., Kossan R., Grzesiuk A., Debski H. Veg Crops Res Bull., 2008, vol. 68, pp. 5–22.
Ng J., Smith S.D. AoB PLANTS, 2016, vol. 8, plw013; doi:10.1093/aobpla/plw013.
Bolotov V.M., Nechaev A.P., Sarafanova L.A. Pishchevye krasiteli: klassifikatsiia, svoistva, analiz, primenenie. [Food colors: classification, properties, analysis, application]. St. Petersburg, 2008, 240 p. (in Russ.).
Savvin P.N., Komarova E.V., Bolotov V.M., Shichkina E.S. Khimiia rastitel'nogo syr'ia, 2010, no. 4, pp. 135–138. (in Russ.).
Oszmianski J., Sapis J. J. Food Sci., 1988, vol. 53, no. 4, pp. 1241–1242.
Veberic R., Jacopic J., Stampar F., Schmitzer V. Food Chem., 2009, vol. 114, pp. 511–515.
Petrova S.N., Kuznetsova A.A. Khimiia rastitel'nogo syr'ia, 2014, no. 4, pp. 43–50. (in Russ.).
Flamini R., Tomasi D. Vitis., 2000, vol. 39, no. 2, pp. 79–81.
Zhao D.Q., Wei M.R., Liu D., Tao J. Plant Physiol. Biochem., 2016, vol. 102, pp. 97–106.
Jia N., Shu Q-Y., Wang D-H., Wang L-S., Liu Z-A., Ren H-X. J. Amer. Soc. Hort. Sci., 2008, vol. 133, no. 3, pp. 418–426.
Hosoki T., Seo M. Bull. Fac. Agr. Shimane Univ., 1991, vol. 25, pp. 11–14.
Deineka L., Deineka V., Tokhtar V., Martynova N. RJPBCS, 2015, vol. 6, no. 5, pp. 944–950.
Lee J.H., Lee H.J., Choung M.G. Food Chem., 2011, vol. 129, no. 2, pp. 272–278.
Ogata J.U.N., Sakamoto T., Yamaguchi M., Kawanobu S., Yoshitama K. J. Plant Physiol., 2001, vol. 158, no. 6, pp. 709–714.
Harborne J.B., Boardley M. Z. Naturforsch., 1985, pp. 305–308.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
