THE RESEARCH OF THE SPECTRUM OF BIOLOGICALLY ACTIVE FLAVONOIDS GRASS HYPERICUM PERFORA-TUM L. FLORA OF THE REPUBLIC OF TATARSTAN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Abstract
The article presents the results of the study of the dependence of the flavonoid content of the herb Hypericum perforatum L., from growth conditions of cenopopulations in various ecological, cenotic conditions of the Republic of Tatarstan. Plant biocenosis were considered – meadows, Border of broadleaf and pine forest or artificial planting of pine. A qualitative and quantitative analysis of flavonoids in the herb of Hypericum perforatum was carried out by high-performance liquid chromatography. In studying water-alcohol extracts of H. perforatum L. flavonoids – rutin, quercetin, bisapigenin were identified by HPLC analysis. It is shown that the ecological, cenotic conditions of growth of Hypericum perforatum affect the quantitative content of phenolic compounds. It has been established that in investigated cenopopulations in the territory of the Republic of Tatarstan, rutin is the dominant flavonoid in medicinal plant raw materials. Maximum amount of this compound detected in the samples of Hypericum perforatum confined to the pine biocenosis of Tukaevsky and Drozhzhanovsky districts under significant quantity level of quercetin. In all variants of the experiment, bisapiginin was detected in small amounts. Plants of Hypericum perforatum, grow in different plant biocenosis differed in the various contents of the compounds of the phenolic complex, which is probably due to the ecological, cenotic and climatic conditions of growth.
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Baidullauly A., Shevchenko A.S., Muzychkina R.A. Lekarstvennye rasteniia: fundamental'nye i prikladnye pro-blemy. Materialy I Mezhdunarodnoi nauchnoi konferentsii. [Medicinal plants: fundamental and applied problems. Materials of the I International Scientific Conference]. Novosibirsk, 2013, pp. 127–130. (in Russ.).
Savchenko L.N., Marinina T.F., Karpenko V.A. Izvestiia Samarskogo nauchnogo tsentra RAN, 2015, vol. 17, no. 5, pp. 197–202. (in Russ.).
Dubrovnaia S.A., Khusnetdinova L.Z. Izvestiia Samarskogo nauchnogo tsentra RAN, 2016, vol. 18, no. 2(2), pp. 355–359. (in Russ.).
Samylina I.A. Farmakognoziia. Uchebnaia praktika [Pharmacognosy. Training Practice], ed. I.A. Samylina, A.A. So-rokina, Moscow, 2011, 432 p. (in Russ.).
Pravdivtseva O.E., Kurkin V.A. Khimiia rastitel'nogo syr'ia, 2008, no. 1, pp. 81–86. (in Russ.).
Zimina L.N., Kurkin V.A., Ryzhov V.M. Khimiia rastitel'nogo syr'ia, 2013, no. 1, pp. 205–208. (in Russ.).
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