THE INFLUENCE OF ULTRASONIC TREATMENT ON THE EXTRACTION OF BIOLOGICALLY ACTIVE COMPOUNDS OF PLANTS OF THE FAMILY CARYOPHYLLACEAE
Abstract
The influence of ultrasonic irradiation on extraction of secondary metabolites from plants of the family Caryophyllaceae was study. It is shown that while carrying out extraction and the effects of ultrasound observed acceleration of the extraction process such biologically active compounds as ecdysteroids and flavonoids. The time of extraction of ecdysteroids from the aerial part of Silene viridiflora, Silene sendtneri, Silene roemeri, colpophylla Silene, Silene frivaldszkyana and Lychnis chalcedonica decreased by 6–12 times. Found that ultrasound has a different effect on the process of extraction of compounds from different plant species. Lychnis chalcedonica, with an average level of ecdysteroids, after 60 minutes of exposure is achieved by the extraction of the ecdysteroids is equal to the control, and after 90 min, exceeding its level by 49%. From Silene frivaldszkyana - species with high content of ecdysteroids control level were achieved after 120 min of ultrasonic treatment. High-intensity ultrasound accelerates the process, reduces the volume of solvents and increases the yield of flavonoids in 28–41% in comparison with the control. The destructive effect of ultrasound on the secondary metabolites not observed, as confirmed by UV-spectra and high performance liquid chromatography.
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