ANTIMICROBIAL ACTIVITY OF MEDICINAL PLANT EXTRACTS ANDROMEDA POLYFOLIA AND ALCHEMILLA SUBCRENATA
Abstract
Plants accumulate in their tissues powerful arsenal of protective substances necessary for survival in the face of abiotic environmental and in aggressive neighborhood with pathogenic bacteria and viruses. We examined the following kinds of medicinal plants: Alchemilla subcrenata and Polyfolia andromeda. Conducted chemical analyses of water and 40 and 70% alcohol extracts of these plants. The content of flavonoids was significantly greater in all ways of extraction of Alchemilla subcrenata compared to Andromeda polyfolia. To the extent the release of phenolic compounds and water-soluble sugars affected the presence of alcohol. From Andromeda polyfolia greater variety of phenolic compounds left in the water and 40% alcohol, and from Alchemilla subcrenata in 40% and 70% ethanol. Extracts compared on the effects on survival and biofilm formation of Escherichia coli XL1-Blue and Pectobacterium carotovorum. Found that extracts of plants studied have varying degrees of antimicrobial action. Alcohol extracts of Andromeda polyfolia suppressed the formation of biofilms of P. carotovorum and E. coli. All extracts of Alchemilla subcrenata stimulated bacterial growth and biofilm formation. The most effective proved to be 70% alcoholic extract of Alchemilla subcrenata.
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Nascimento P.L.A., Nascimento T.C.E.S., Ramos N.S.M., Silva G.R., Gomes J.E.G., Falcao R.E.A., Moreira K.A., Porto A.L.F, Silva T.M.S. Quantification, antioxidant and antimicrobial activity of phenolics isolated from different extracts of Capsicum frutescens (pimenta malagueta). // Molecules. 2014. Vol. 19. P. 5434–5447.
Nazzaro F., Fratianni F., Coppola R. Quorum sensing and phytochemicals // International journal of molecular sciences. 2013. Vol. 14, N 6. Р. 12607–12619.
Zhang N., Wang D., Liu Y. Effects of different plant root exudates and their organic acid components on chemotaxis, biofilm formation and colonization by beneficial rhizosphere-associated bacterial strains // Plant and Soil. 2014. Vol. 374, N 1-2. Р. 689–700.
Храмова Е.П., Цыбуля Н.В., Чиндяева Л.Н. Антимикробная активность летучих соединений и содержание фенольных компонентов у некоторых видов рода Pentaphylloides (Rosaceae) // Растительные ресурсы. 2013. Т. 49. С. 598–612.
Costerton J.W., Stewart P.S., Greenberg E.P. Bacterial biofilms: a common cause of persistent infections // Science. 1999. Vol. 284. P. 1318- 1322.
Nickel J.C., Wright J.B., Ruseska I., Marrie T.J., Whitfield C., Costerton J.W. Antibiotic resistance of Pseudomonas aeruginosa colonizing a urinary catheter in vitro // European Journal of Clinical Microbiology. 1985 Vol. 4. P. 213–218. doi:10.1007/BF02013600.
Borriello G., Werner E., Roe F., Kim A.M., Ehrlich G.D., Stewart P.S. Oxygen limitation contributes to antibiotic tolerance of Pseudomonas aeruginosa in biofilms // Antimicrobial Agents and Chemotherapy. 2004. Vol. 48. P. 2659–2664. doi:10.1128/AAC.48.7.2659-2664.2004.
Kim Y.I., Lee Y.H., Kim K.H., Oh Y.K., Moon Y.H., Kwak W.S. Effects of Supplementing Microbially-fermented Spent Mushroom Substrates on Growth Performance and Carcass Characteristics of Hanwoo Steers (a Field Study) // Asian-Australasian Journal of Animal Sciences. 2012. Vol. 25, N 11. P. 1575-1581. doi: 10.5713/ajas.2012.12251.
Телятьев В.В. Полезные растения Центральной Сибири. Иркутск: Восточно-Сибирское книжное издательство, 1985. 384 с.
Зорина Е.В. Фармакогностическое изучение видов рода Alchemilla L. Пермского края : автореф. дис. … канд. фармацевтических наук. Пермь, 2009. 21 с.
Верещагин В.И., Соболевская К.А., Якубова А.И. Полезные растения Западной Сибири. 1959. М.: Наука. 346 с.
Filippova E.I. Antiviral Activity of Lady's Mantle (Alchemilla vulgaris L.) Extracts against Orthopoxviruses. // Bulletin of Experimental Biology and Medicine. 2017. Vol. 163, N 3. P. 374-377. doi: 10.1007/s10517-017-3807-x.
Karatoprak G.S., İlgün S., Koşar M. Phenolic Composition, Anti-Inflammatory, Antioxidant, and Antimicrobial Activities of Alchemilla mollis (Buser) Rothm // Chemistry & Biodiversity. 2017. Vol. 14, N 9. doi: 10.1002/cbdv.201700150.
Raza S.A., Rashid A., William J., Razzaq A. Evaluation of oxidative stability of sunflower oil at frying temperature in presence of butylated hydroxytoluene and methanolic extracts of medicinally important plants of Pakistan // International Food Research Journal. 2014. Vol. 21, N 1. P. 331-334.
Mansour E., Khaled A.B., Lachiheb B., Abid M., Bachar Kh., Ferchichi A. Phenolic compounds, antioxidant, and antibacterial activities of peel extract from Tunisian pomegranate // Journal of Agricultural Science and Technology. 2013. Vol. 15. P. 1393-1403.
Марданова А.М., Кабанов Д.А., Рудакова Н.Л., Шарипова М.Р. Биопленки: основные методы исследования: учебно-методическое пособие / Научный редактор: проф., д.б.н. О.Н. Ильинская. Казань: К(П)ФУ, 2016. 42 с.
Sepulveda-Jimenez G., Reyna-Aquino С., Chaires-Martinez L., Bermudez-Torres K., Rodriguez-Monroy M. Antioxidant activity and content of phenolic compounds and flavonoids from Justicia spicigera // Journal of Biological Sciences. 2009. Vol. 9, N 6. P. 629-632.
Герман А., Боченек Ж., Герман А.П. Действие масел корицы и лаванды на экспрессию гена FtsZ Staphylococcus aureus ATCC 29213 // Прикладная биохимия и микробиология. 2013. Т. 49, № 5. С. 476-480.
Тараховский Ю.С., Ким Ю.А., Абдрасилов Б.С., Музафаров Е.Н. Флавоноиды: биохимия, биофизика, медицина. / Отв. ред. Е.И. Маевский. Пущино: Sуnchrobook, 2013. 310 c.
Bogino P.C., Oliva M. de la M., Sorroche F.G., Giordano W. The role of bacterial biofilms and surface component in plant-bacterial associations // International Journal of Molecular Sciences. 2013. N. 14. P. 15838-15859.
Sankar Ganesh P., Ravishankar Rai V. J. Attenuation of quorum-sensing-dependent virulence factors and biofilm formation by medicinal plants against antibiotic resistant Pseudomonas aeruginosa // Journal of Traditional and Complementary Medicine. 2017. Vol. 8, N 1. P. 170-177. doi: 10.1016/j.jtcme.2017.05.008
Jałowiecki Ł., Żur J., Chojniak J., Ejhed H., Płaza G. Properties of Antibiotic-Resistant Bacteria Isolated from Onsite Wastewater Treatment Plant in Relation to Biofilm Formation // Current Microbiology. 2018. Vol. 20. doi: 10.1007/s00284-017-1428-2.
Максимов А.Ю. Генетические эффекты флавоноидов в бактериальных культурах : автореф. дис. … канд. биол. наук. Пермь, 2004.
Зайцева Ю.В. Молекулярно-генетические особенности Quorum Sensing систем грамотрицательных бактерий (на модели Serratia) и изучение их роли в регуляции клеточных процессов : автореф. дис. … канд. биол. наук. Москва, 2012.
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