COMPONENT COMPOSITION OF ESSENTIAL OIL OF PINE BUDS (PINUS SYLVESTRIS L.), GROWING IN THE URBAN ENVIRONMENT OF TOMSK REGION
Abstract
The component composition of pine buds essential oil, growing in the area of active economic activity in Tomsk region, has been studied. Essential oil from pine buds obtained by the method of distillation with the water steam.
The component composition of the essential oil was studied by gas-liquid-chromatography-mass spectrometry on the gas chromatograph Agilent 6890 with quadrupole mass spectrometer (HP MSD 5973N). Chromatography conditions: 30-meter long quartz column HP-5MS with internal diameter 0.25 mm, film thickness of 0.25 µm (copolymer 5% diphenyl 95% dimethylsiloxane), carrier gas – helium (constant flow 1 ml/min), column temperature – 50 °С (2 min isotherm), 50–200 °С (40 °C, min), 200–280 °С (20 °С/min), 280 °С (5 min isotherm), the temperature of the ion source 170 °С, the interface between the gas chromatograph and mass selective detector 280 °С. The energy of ionizing electrons is 70 eV. The volume of injected sample of 1 µl. The component composition was determined by comparing the times and linear retention indices, mass spectra values, the database of the chromatographic-mass spectrometric library of volatile substances of plant origin. About 163 individual components were found. It is established that with the increase of technogenic pollution, the amount of essential oil decreases, the component composition changes, which can serve as an indicator of the quality of raw materials, the state of the forest stand of pine and the environment as a whole.
Data on the dominant content of 3-caren in the composition of essential oils of the pine buds, growing in the Tomsk region, suggests a limited use of this raw material and essential oil on its basis in medical practice.
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