THE CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES WATER-ALCOHOL EXTRACTS OF ASPEN BARK
UDC 630.813
Abstract
The aim of this work was to study the bark of aspen growing in the suburban area of Krasnoyarsk and obtain water-ethanol extracts based on it, with different growth-regulating activity. The paper deals with the possible processing of aspen bark with the release of biologically active substances of the phenolic group. Water-ethanol solutions of aspen bark of different concentrations were used as an extractant. The regression equations and the optimum conditions of extraction of the bark of aspen with the allocation of the maximum amount of extractives. Studies have shown that aspen bark contains a variety of groups of biologically active substances, including phenolic group: tannins (tannins from 17.0 to 18.0%) polyphenols (glycosides – 0.71–0.84%), flavonoids – 0.33–0.43%; pigments: (chlorophylls from 0.63 to 0.74%; carotenoids (from 0.52 to 0.55%). The obtained mathematical models were adequate to the studied processes at 95% confidence probability. The optimal values for maximum extraction of extractive substances: the concentration of ethanol was 65%; the duration of the process – 3.5 hours, hydro – 15. In the optimal mode was obtained aspen bark extract, its characteristics coincide with the requirements of TU 9377-162-20680882-10 "raw Materials for the production of biologically active additives "aspen bark Extract "thick". It was established that water-ethanol extracts of aspen bark have depending on the concentration of growth-inhibiting or stimulating activity. In the range of concentrations from 0.1 to 10 g/l, they have an inhibitory effect, with further dilution from 1∙10-2 to 1∙10-4 g/l, a stimulating effect is observed. The tendency of increasing the growth of seedlings of cereals, %: corn – 30, barley – 40 and wheat – 35; legumes, %: peas – 35, beans – 45 and lentils – 25 compared with the control at a concentration of extractive substances of aspen bark 1∙10-3 g/l. the Data obtained may be useful after appropriate sanitary-toxicological studies to increase the germination and germination of seeds of legumes and cereals.
Downloads
Metrics
References
Gosudarstvennyy doklad «O sostoyanii i okhrane okruzhayushchey sredy v Krasnoyarskom kraye v 2017 godu». Min-isterstvo ekologii i ratsional'nogo prirodopol'zovaniya Krasnoyarskogo kraya. [State report “On the State and Envi-ronmental Protection in the Krasnoyarsk Territory in 2017: the Ministry of Ecology and Environmental Management of the Krasnoyarsk Territory]. Krasnoyarsk, 2018, 301 p. (in Russ.).
Reyser, G.V., Isayeva Ye.V. Nepreryvnoye ekologicheskoye obrazovaniye i ekologicheskiye problemy. [Continuing En-vironmental Education and Environmental Issues]. Krasnoyarsk, 2004, vol. 3, pp. 99–103. (in Russ.).
Levdanskiy V.A., Polezhayeva N.I., Makiyevskaya A.I., Petukhova N.N., Kuznetsov B.N. Kontseptsiya gomeostaza: teoreticheskiye, eksperimental'nyye i prikladnyye aspekty: sbornik trudov X mezhdunarodnogo Simpoziuma. [The con-cept of homeostasis: theoretical, experimental and applied aspects: a collection of works of the X International Symposi-um]. Novosibirsk, 2002, pp. 60–64. (in Russ.).
Deinefco L.P., Faustova N.M. 12th Intemational Symposimn on Wood and Pulping Chemistry. Madison, 2003, pp. 189–191.
Zaks P.E. Wood and cellulose chemistry. New York, 1991, pp. 253–330.
Kuznetsov B.N., Levdanskiy V.A., Kedrova L.P., Yes'kin A.P., Polezhayeva N.I. et al Khimiya rastitel'nogo syr'ya, 1998, no. 3, pp. 5–12. (in Russ.).
Dolgodvorova S.Ya., Burlakova R.F., Chernyayeva G.N. Khimiya drevesiny, 1990, no. 5, pp. 79–82. (in Russ.).
Faustova N.M. Khimicheskiy sostav kory i drevesiny osiny: diss. … kand. khim. nauk. [The chemical composition of bark and wood of aspen: Diss. ... cand. Chem. of sciences]. St. Petersburg, 2005, 208 p.
Burlakova R.F., Dolgodvorova S.YA., Chernyayeva G.N. Ekstraktivnyye veshchestva drevesnykh rasteniy: tezisy dokladov. [Extractives of woody plants: abstracts]. Novosibirsk, 1986, pp. 107–109 (in Russ.).
Deyneko I.P., Faustova N.M. Khimiya rastitel'nogo syr'ya, 2015, no. 1, pp. 51–62 (in Russ.).
Zaprometov M.N. Fenol'nyye soyedineniya: rasprostraneniye, metabolizm i funktsii v rasteniyakh. [Phenolic com-pounds: distribution, metabolism and functions in plants]. Moscow, 1993, 272 p. (in Russ.).
Kozlov Yu.V., Lyakina O.A., Samsonova N.Ye., Kuz'minskaya V.A. Agrokhimiya i ekologiya: istoriya i sovremen-nost': materialy Mezhdunarodnoy nauchno-prakticheskoy konferentsii. [Agricultural chemistry and ecology: history and modernity: materials of the International scientific-practical conference]. Nizhny Novgorod, 2008, vol. 2, pp. 109–113 (in Russ.).
Taraban'ko V.Ye., Ul'yanova O.A., Kalacheva G.S. Khimiya rastitel'nogo syr'ya, 2010, no. 1, pp. 121–126. (in Russ.).
Soboleva S.V., Chentsova L.I., Voronin V.M. Pererabotka kory osiny s polucheniyem biologicheski aktivnykh vesh-chestv i kormovykh produktov: monografiya. [Processing aspen bark to produce biologically active substances and feed products: monograph]. Krasnoyarsk, 2013, 77 p. (in Russ.).
Obolenskaya A.V., Yel'nitskaya Z.P., Leonovich A.A. Laboratornyye raboty po khimii drevesiny i tsellyulozy: uchebnoye posobiye. [Laboratory work on the chemistry of wood and cellulose: a training manual]. Mosocw, 1991, 320 p. (in Russ.).
Ryazanova T.V., Chuprova N.A., Isayeva Ye.V. Khimiya drevesiny. [Wood chemistry]. Krasnoyarsk, 1996, 358 p. (in Russ.).
Ushanova V.M., Lebedeva O.I., Devyatlovskaya A.N. Osnovy nauchnykh issledovaniy: uchebnoye posobiye. Ch. 3: Is-sledovaniye khimicheskogo sostava rastitel'nogo syr'ya. [Fundamentals of research: a training manual. Part 3: Study of the chemical composition of plant materials]. Krasnoyarsk, 2004, 360 p. (in Russ.).
GOST 12038-84 Semena sel'skokhozyaystvennykh kul'tur. Metody opredeleniya vskhozhesti. [GOST 12038-84 Seeds of crops. Germination Methods]. Moscow, 2011, 15 p. (in Russ.).
Soboleva S.V., Chentsova L.I., Pochekutov I.S. Vestnik KrasGAU, 2012, no. 9, pp. 215–218 (in Russ.).
![](http://journal.asu.ru/public/journals/1/article_4442_cover_ru_RU.jpg)
Copyright (c) 2020 Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.