DEVELOPMENT OF METHODS FOR OBTAINING POLYSACCHARIDES FROM BIRCH LEAVES (BETULA PENDU-LA ROTH., BETULA PUBESCENS EHRH.)
Abstract
The development of a complex technique for the extraction and purification of polysaccharides from birch leaves (Betula pendula Roth.,Betula pubescens Ehrh.) (PSfB) was the purpose of this study. The following characteristics were used as criteria for evaluating the effect of the parameters studied on the production of target substances: yield of PSfB (gravimetric method), protein content (spectrophotometric method), molecular weight distribution (high performance exclusion chromatography) and degree of purification from low molecular weight impurities (LMWI) (IR) spectroscopy).During the experiment, the optimal parameters were determined at various stages of obtaining the PSfB: degree of raw materials grinding – 1.2–3 mm, pH of the extractant (purified water with pH=7), the ratio of raw materials : extractant (1 : 20), extraction temperature (50 °C), temperature and degree of evaporation (50 °C; 4 times), the ratio of concentrate : ethanol (1 : 3) and the method of purification from the LMWI (ultrafiltration).The result of this work is an optimized method for obtaining PSfB, allowing to achieve high yield without loss of product quality (minimum allowable content of low molecular weight impurities with the highest content of high molecular weight fractions).This technique is the basis for the development of laboratory regulations for obtaining an active pharmaceutical substance based on polysaccharides from birch leaves.
Downloads
Metrics
References
Yeates K., Lohfeld L., Sleeth J., Morales F., Rajkotia Y., Ogedegbe O. Can. J. Cardiol., 2015, vol. 31, no. 9, pp. 1081–1093, DOI: 10.1016/j.cjca.2015.06.035.
Nakamura M., Miura S., Takagaki A., Nanjo F. Int. J. Food SciNutr., 2017, vol. 68, no. 3, pp. 321–330, DOI: 10.1080/09637486.2016.1232376.
Hebi M, Eddouks M. J. Integr. Med., 2017, vol. 15, no. 6, pp. 476–482, DOI: 10.1016/S2095-4964(17)60361-3.
Korolenko T.A., Johnston T.P., Machova E., Bgatova N.P., Lykov A.P., Goncharova N.V., Nescakova Z., Shintyapina A.B., Maiborodin I.V., Karmatskikh O.L. International Journal of Biological Macromolecules, 2018, vol. 107, part B, pp. 2385–2394, DOI: 10.1016/j.ijbiomac.2017.10.111.
Shituleni A., Gan F., Nido S.A., Mengistu B.M., Khan A.Z., Liu Y., Huang K. Carbohydrates and Dietary Fibre, 2016, vol. 8, no. 2, pp. 51–57, DOI: 10.1016/j.bcdf.2016.10.001.
Shukshina O.G. Sbornik materialov I Vserossiyskaya nauchnaya studencheskaya konferentsiya s mezhdunarodnym uchastiyem Mediko-biologicheskiye nauki: dostizheniya i perspektivy. [Collection of materials I All-Russian Scientific Student Conference with international participation Biomedical Sciences: achievements and prospects]. Tomsk, 2011, pp. 127–129. (in Russ.).
Aspinall G.O. The Polysacharides, Academic Press, 1983, 518 p. DOI: 10.1016/C2013-0-10317-0.
Khasanova S.R., Krivoshchekov S.V., Kudashkina N.V., Gur'yev A.M., Rovkina K.I., Belousov M.V. Rastitel'nyye resursy, 2015, vol. 51, no. 3, pp.397–406. (in Russ.).
Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. J. Biol. Chem, 1951, vol. 193, pp. 265–275.
Rovkina K.I., Krivoshchekov S.V., Guryev A.M., Yusubov M.S., Belousov M.V. Russ. J. Bioorg. Chem, 2018, vol. 44, no. 7, pp. 854–859, DOI: 10.1134/S1068162018070105.
Sal'nikova Ye.N., Kalinkina G.I., Dmitruk S.Ye. Khimiya rastitel'nogo syr'ya, 2001, no. 3, pp. 71–78. (in Russ.).
Krasochko P.A., Kaputskiy F.N., Krasochko I.A., Zubets O.V., Alad'yeva T.A. Uchenyye Zapiski UO VGAVM, 2012, vol. 48, no. 2, part I, pp. 84–87. (in Russ.).
Domozych D.S., Sørensen I., Popper Z.A., Ochs J., Andreas A., Fangel J.U., Pielach A., Sacks C., Brechka H., Ruisi-Besares P., Willats W.G.T., Rose J.K.C. Plant, 2014, vol. 165, no. 1, pp. 105–118, DOI: 10.1104/pp.114.23625.
Levin B.D., Fedyulin A.S. Vestnik KrasGAU, 2007, no. 2, pp. 266–269. (in Russ.).
Liu J., Bai R., Liu Y., Zhang X., Kan J., Jin C. Int. J. Biol. Macromol, 2018, vol. 107, part B, pp. 2242–2250, DOI: 10.1016/j.ijbiomac.2017.10.097.
Sukhov B.G., Pogodaeva N.N., Kuznetsov S.V., Kupriyanovich Yu.N., Yurinova G.V., Selivanova D.S., Pistav-ka A.A., Dzhioev Yu.P., Popkova S.M., Rakova E.B., Medvedeva P.A., Trofimov B.A. Russ. Chem. Bull., 2014, vol. 63, no. 9, pp. 2189–2194, DOI: 10.1007/s11172-014-0718-0.
Shipovskaya A.B. Metody vydeleniya i fiziko-khimicheskiye svoystva prirodnykh polisakharidov. [Isolation methods and physicochemical properties of natural polysaccharides]. Saratov, 2015. 64 p. (in Russ.).
Fan L.P., Li J.W., Deng K.Q., Ai L.Z. Carbohydr. Polym., 2012, vol. 87, pp. 1849–1854, DOI: 10.1016/j.carbpol.2011.10.018.
Li X.Y., Wang L., Wang Y., Xiong Z.H. Process Biochem., 2016, vol. 51, pp. 1100–1108, DOI: 10.1016/j.procbio.2016.05.006.
Warrand J., Michaud P., Miller G., Courtois D., Ralainirina R. Chromatographia, 2003, vol. 58, pp. 331–335, DOI: 10.1365/s10337-003-0060-4.
Lu X., Li N., Qiao X., Qiu Z., Liu P. LWT, 2018, vol. 95, pp. 223–229, DOI: 10.1016/j.lwt.2018.04.059.
Jiang Y., Qi X., Gao K., Liu W., Li N., Cheng N. Glycoconj. J., 2016, vol. 33, pp. 755–761, DOI: 10.1007/s10719-016-9669-z.
Copyright (c) 2019 Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
