THE INFLUENCE OF HUMIC ACIDS ON THE SOWING QUALITIES OF COCKWEED IN OIL POLLUTION
UDC 544.77.052.5
Abstract
The stimulating effect of humic acids on the processes of growth and development of cress seeds has been revealed. It has been established that use of humic acids of black alder fen peat most effectively affects the sowing qualities of seeds under conditions of oil pollution (an increase in the germination energy and germination capacity compared to the non-humic acid variant by 38%). The minimal inhibitory effect on shoot growth was observed in the soil with hexadecane (20.8 mm less than the control), the maximum – in the soil with oil (22.5 mm less than the control). Humic acids show a stimulating effect on the growth of cress roots under conditions of pollution with oil hydrocarbons. The root length indicators exceeded the values obtained in the version with the pollutant without the use of humic acids from 6% (oil) to 16% of the control (hexadecane). Based on the data obtained during the vegetation experiments, it can be assumed that the stimulating activity of humic acids is more pronounced in stressful conditions for the organism. The biostimulating effect of humic acids was revealed, which manifested itself in an increase in the length of the hypocotyl and root, and the physiological activity of detoxicants was more effectively detected under conditions of pollution with oil hydrocarbons. According to the results of the vegetative experience on a substrate with hexadecane during the irrigation with humic acids of sphagnum high-moor peat, the maximum values of the test responses were determined, corresponding to the control values obtained on the uncontaminated substrate, or higher than the latter. In the variant with oil, the most pronounced effect was achieved with the introduction of humic acids of reed fen and sphagnum high-moor peat, which confirms the hypothesis about the protective effect of humic acids in pollution conditions, which is determined by the binding ability of humic acids in relation to toxicant and their own biological activity in the relation to plants. It is proved that humic acids of peats make a significant contribution to the natural processes of self-purification, causing immobilization and detoxification of petroleum hydrocarbons, which indicates the prospects of their application in technologies of purification and recultivation of polluted water and soil media.
Downloads
Metrics
References
Vagapova M.N., Miroshnichenko A.A. Molodezhnyy nauchnyy forum: Yestestvennyye i meditsinskiye nauki: elektr. sbornik statey po materialam XXXVIII mezhdunarodnoy studencheskoy nauchno-prakticheskoy konferentsii. [Youth Scientific Forum: Natural and Medical Sciences: electr. collection of articles based on materials of the XXXVIII interna-tional student scientific-practical conference], Moscow, 2016, no. 9(37), pp. 73–76. (in Russ.).
Davydova S.L., Tagasov V.I. Neft' i nefteprodukty v okruzhayushchey srede: uchebnoye posobiye. [Petroleum and pe-troleum products in the environment: a training manual]. Moscow, 2004, 163 p. (in Russ.).
Demin V.V., Zavgorodnyaya Yu.A., Terent'yev V.A. Doklady po ekologicheskomu pochvovedeniyu, 2006, vol. 1, no. 1, pp. 72–79. (in Russ.).
Boykova O.I., Volkova Ye.M. Izvestiya TulGU. Yestestvennyye nauki, 2013, no. 3, pp. 253−264. (in Russ.).
Akatova Ye.V., Dmitriyeva Ye.D., Syundyukova K.V., Leont'yeva M.M., Muzafarov Ye.N. Khimiya rastitel'nogo syr'ya, 2017, no. 1, pp. 119−127. DOI: 10.14258/jcprm.2017011382 (in Russ.).
Dmitriyeva Ye.D., Syundyukova K.V., Akatova Ye.V., Leont'yeva M.M., Volkova Ye.M., Muzafarov Ye.N. Khimiya rastitel'nogo syr'ya, 2017, no. 1, pp. 137–144. DOI: 10.14258/jcprm.2017011418 (in Russ.).
Dmitriyeva Ye.D., Leont'yeva M.M., Osina K.V. Vestnik TvGU. Seriya: Khimiya, 2019, no. 1(35), pp. 134–146. DOI: 10.26456/vtchem2019.1.17 (in Russ.).
Saleyem K.M. Ispol'zovaniye guminovykh preparatov dlya detoksikatsii i biodegradatsii neftyanogo zagryazne-niya: avtoref. dis. … kand. khim. nauk. [The use of humic preparations for detoxification and biodegradation of oil pollution: abstract. dis. ... cand. chem. sciences]. Moscow, 2004, 30 p. (in Russ.).
Arene V.Zh., Saushkin A.Z., Gridin O.M., Gridin A.O. Ochistka okruzhayushchey sredy ot uglevodorodnykh za-gryazneniy. [Cleaning the environment from hydrocarbon pollution]. Moscow, 1999, 371 p. (in Russ.).
Sikkema J.B., Poolman B.В. Microbiology, 1995, vol. 59, pp. 201–222.
Cunningham C.J., Philp J.C. Land Contamination & Reclamation, 2000, vol. 8, no. 4, pp. 261–269.
Nazarov A.V. Vestnik Permskogo gosudarstvennogo universiteta. Biologiya, 2007, vol. 5 (10), pp. 134–141. (in Russ.).
Minai-Tehrani D., Herfatmanesh A. Bioremediation Journal, 2007, vol. 11, no. 2, pp. 71–76. DOI: 10.1080/10889860701351589
Donets Ye.V. Vliyaniye nefti na prorastaniye semyan khvoynykh lesoobrazuyushchikh vidov drevesnykh rasteniy pod zony yuzhnoy taygi Omskoy oblasti: dis. … kand. biol. nauk. [The effect of oil on seed germination of coniferous forest-forming species of woody plants under the zone of the southern taiga of the Omsk region: dis. ... cand. biol. sciences]. Omsk, 2009, 148 p. (in Russ.).
Ogbo E.M. African Journal of Biotechnology, 2009, vol. 8, no. 2, pp. 250–253.
Demin V.V., Biryukov M.V., Semenov A.A. Doklady po ekologicheskomu pochvovedeniyu, 2006, vol. 1, no. 1, pp. 80–91. (in Russ.).
Dunstone R.L., Richards R.A., Rawson H.M. Australian Journal of Agricultural Research, 1988, vol. 39, no. 4, pp. 547–553. DOI: 10.1071/AR9880547.
Yamada P., Asou K., Yazawa Y., Yamaguchi T. Japanese Journal of Soil Science and Plant Nutrition, 2002, vol. 73, no. 6, pp. 777–781. DOI: 10.20710/dojo.73.6_777
Kulikova N.A., Dashitsyrenova A.D., Perminova I.V., Lebedeva G.F. Bulgarian journal of ecological science, 2003, vol. 2, no. 3–4, pp. 55–56.
Bobyr' L.F. Guminovyye udobreniya. Teoriya i praktika ikh primeneniya, 1980, vol. 7, pp. 54–63. (in Russ.).
Grechishcheva N.Yu. Razrabotka nauchnykh osnov primenneniya guminovykh veshchestv dlya likvidatsii posledstviy netezagryazneniya pochvennykh i vodnykh sred: diss. … kand. khim. nauk. [Development of the scientific basis for the use of humic substances to eliminate the effects of non-pollution of soil and water: diss. ... cand. chem. sciences]. Moscow, 2016, 326 p. (in Russ.).
Kulikova N.A., Perminova I.V., Lebedeva G.F., Matorin D.N. Vestnik Moskovskogo universiteta, seriya 16 (Biologiya), 1997, no. 2, pp. 36–41. (in Russ.).
Liang Y.N., Britt D.W., McLean J.E. Applied Microbiology and Biotechnology, 2007, vol. 74, no. 6, pp. 1368–1375. DOI: 10.1007/s00253-006-0769-8.
![](http://journal.asu.ru/public/journals/1/article_5521_cover_ru_RU.png)
Copyright (c) 2019 Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.