DEVELOPMENT OF A DRUG BASED ON THE FRUITS OF CHOKEBERRY (ARÓNIA MELANOCÁRPA (MICHX.) EL-LIOT) IMPROVING THE EFFICIENCY OF CANCER CHEMOTHERAPY
UDC 615.277.3:616-006-036.8:582.711.71
Abstract
Black chokeberry is an official drug raw material and is used as a multivitamin drug. Black chokeberry guarantees the supply of raw materials as it grows all over the Russian Federation end is a promising sourse of phenolic compounds for new drugs.
Objective: to develop the most favorable conditions for extraction of bioactive compounds from black chokeberry and to assess its anti-metastatic activity.
To extract bioactive compounds from black chokeberry the method of multistep repercolation with completed cycle was used. To assess the factors controlling the raw material extraction the technique of mathematic simulation for latin square. The technique of liquid anthocyanins and catechins rich black chokeberry extraction was given with the following parameters: extragent – 95% acidified alcohol, phase relation – 1 : 5, number of percolators – 5.
The content of biologically active substances in the extract obtained with 95% acidified ethyl alcohol was determined by conventional methods. In the composition of the extract, the content of anthocyanins (5.83±0.25%), catechins (0.22±0.02%), tannins (4.65±0.30%), phenolic acids (0.27±0.01%) and other biologically active substances was determined. To assess the antitumor properties of the chokeberry fruit extract, the Lewis lung carcinoma model was used. It was found that the proposed extract reliably inhibits the development of metastases and increases the antimetastatic activity of cyclophosphamide in combined treatment, is of interest as a means for increasing the effectiveness of tumor chemotherapy.
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